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Article: Nucleic acid-based cross-linking assay for detection and quantification of hepatitis B virus DNA

TitleNucleic acid-based cross-linking assay for detection and quantification of hepatitis B virus DNA
Authors
Issue Date1999
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 1999, v. 37 n. 1, p. 161-164 How to Cite?
AbstractA nucleic acid photo-cross-linking technology was used to develop a direct assay for the quantification of hepatitis B virus (HBV) DNA levels in serum. Cross-linker-modified DNA probes complementary to the viral genomes of the major HBV subtypes were synthesized and used in an assay that could be completed in less than 6 h. The quantification range of the assay, as determined by testing serial dilutions of Eurohep HBV reference standards and cloned HBV DNA, was 5 x 105 to 3 x 109 molecules of HBV DNA/ml of serum. Within-run and between-run coefficients of variation (CVs) for the assay were 4.3 and 4.0%, respectively. The assay was used to determine HBV DNA levels in 302 serum samples, and the results were compared to those obtained after testing the same samples with the Chiron branched-DNA (bDNA) assay for HBV DNA. Of the samples tested, 218 were positive for HBV DNA by both assays and 72 gave results below the cutoff for both assays. Of the remaining 12 samples, 10 were positive for HBV DNA by the cross-linking assay only; the 2 other samples were positive by the bDNA assay only. Twenty-eight samples had to be retested by the bDNA assay (CV, >20% between the results obtained from the testing of each sample in duplicate), whereas only three samples required retesting by the cross-linking assay. The correlation between the HBV DNA levels, as measured by the two tests, was very high (r = 0.902; P = 0.01). We conclude that the cross-linking assay is a sensitive and reproducible method for the detection and quantification of HBV DNA levels in serum.
Persistent Identifierhttp://hdl.handle.net/10722/49098
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, VCHen_HK
dc.contributor.authorGuan, Ren_HK
dc.contributor.authorWood, MLen_HK
dc.contributor.authorLo, SKen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorLai, CLen_HK
dc.date.accessioned2008-06-12T06:34:22Z-
dc.date.available2008-06-12T06:34:22Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 1999, v. 37 n. 1, p. 161-164en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49098-
dc.description.abstractA nucleic acid photo-cross-linking technology was used to develop a direct assay for the quantification of hepatitis B virus (HBV) DNA levels in serum. Cross-linker-modified DNA probes complementary to the viral genomes of the major HBV subtypes were synthesized and used in an assay that could be completed in less than 6 h. The quantification range of the assay, as determined by testing serial dilutions of Eurohep HBV reference standards and cloned HBV DNA, was 5 x 105 to 3 x 109 molecules of HBV DNA/ml of serum. Within-run and between-run coefficients of variation (CVs) for the assay were 4.3 and 4.0%, respectively. The assay was used to determine HBV DNA levels in 302 serum samples, and the results were compared to those obtained after testing the same samples with the Chiron branched-DNA (bDNA) assay for HBV DNA. Of the samples tested, 218 were positive for HBV DNA by both assays and 72 gave results below the cutoff for both assays. Of the remaining 12 samples, 10 were positive for HBV DNA by the cross-linking assay only; the 2 other samples were positive by the bDNA assay only. Twenty-eight samples had to be retested by the bDNA assay (CV, >20% between the results obtained from the testing of each sample in duplicate), whereas only three samples required retesting by the cross-linking assay. The correlation between the HBV DNA levels, as measured by the two tests, was very high (r = 0.902; P = 0.01). We conclude that the cross-linking assay is a sensitive and reproducible method for the detection and quantification of HBV DNA levels in serum.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 1999, v. 37 n. 1, p. 161-164en_HK
dc.subject.meshDNA Probesen_HK
dc.subject.meshDNA, Viral - blooden_HK
dc.subject.meshHepatitis B virus - genetics - isolation & purificationen_HK
dc.subject.meshCross-Linking Reagentsen_HK
dc.subject.meshEvaluation Studies as Topicen_HK
dc.titleNucleic acid-based cross-linking assay for detection and quantification of hepatitis B virus DNAen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=37&issue=1&spage=161&epage=164&date=1999&atitle=Nucleic+acid-based+cross-linking+assay+for+detection+and+quantification+of+hepatitis+B+virus+DNAen_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9854083en_HK
dc.identifier.pmcidPMC84196en_HK
dc.identifier.scopuseid_2-s2.0-0032934245en_HK
dc.identifier.hkuros44916-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032934245&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue1en_HK
dc.identifier.spage161en_HK
dc.identifier.epage164en_HK
dc.identifier.isiWOS:000077587900030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, VCH=7006205374en_HK
dc.identifier.scopusauthoridGuan, R=7102456913en_HK
dc.identifier.scopusauthoridWood, ML=7403448361en_HK
dc.identifier.scopusauthoridLo, SK=7401542308en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK

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