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Article: Rapid cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence staining

TitleRapid cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence staining
Authors
Issue Date1998
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 1998, v. 36 n. 3, p. 638-640 How to Cite?
AbstractA rapid cytomegalovirus (CMV) pp65 antigenemia assay with direct erythrocyte lysis (DL) with 0.8% NH4Cl, followed by indirect immunofluorescence staining (IF), was evaluated with 82 blood samples from renal transplant recipients, and the results were compared to those of the conventional antigenemia assay with dextran sedimentation and two-cycle alkaline phosphatase, anti-alkaline phosphatase staining (DS-APAAP). The DL- IF modification gave a higher leukocyte yield compared to DS-APAAP (75.4 versus 54.9%; P < 0.05), with similar leukocyte viability rates of >95%. The DL-IF methodology involved fewer technical steps, and the assay time was shortened from 5 h to less than 3 h. Nineteen of the 82 samples concordantly tested positive for pp65 antigenemia by both assays, and the readings showed a good correlation (r = 0.996; P < 0.01). No discordant results were observed. We conclude that the CMV pp65 antigenemia assay by this novel DL- IF modification is technically simpler, cheaper, and less time-consuming but yields results comparable to those of the conventional DS-APAAP assay. The shortened assay time and increased capacity to handle more samples confer distinct advantages in the rapid diagnosis and prompt treatment of CMV disease in immunosuppressed patients.
Persistent Identifierhttp://hdl.handle.net/10722/49072
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, SKNen_HK
dc.contributor.authorLo, CYen_HK
dc.contributor.authorCheng, IKPen_HK
dc.contributor.authorChan, TMen_HK
dc.date.accessioned2008-06-12T06:33:48Z-
dc.date.available2008-06-12T06:33:48Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 1998, v. 36 n. 3, p. 638-640en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49072-
dc.description.abstractA rapid cytomegalovirus (CMV) pp65 antigenemia assay with direct erythrocyte lysis (DL) with 0.8% NH4Cl, followed by indirect immunofluorescence staining (IF), was evaluated with 82 blood samples from renal transplant recipients, and the results were compared to those of the conventional antigenemia assay with dextran sedimentation and two-cycle alkaline phosphatase, anti-alkaline phosphatase staining (DS-APAAP). The DL- IF modification gave a higher leukocyte yield compared to DS-APAAP (75.4 versus 54.9%; P < 0.05), with similar leukocyte viability rates of >95%. The DL-IF methodology involved fewer technical steps, and the assay time was shortened from 5 h to less than 3 h. Nineteen of the 82 samples concordantly tested positive for pp65 antigenemia by both assays, and the readings showed a good correlation (r = 0.996; P < 0.01). No discordant results were observed. We conclude that the CMV pp65 antigenemia assay by this novel DL- IF modification is technically simpler, cheaper, and less time-consuming but yields results comparable to those of the conventional DS-APAAP assay. The shortened assay time and increased capacity to handle more samples confer distinct advantages in the rapid diagnosis and prompt treatment of CMV disease in immunosuppressed patients.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 1998, v. 36 n. 3, p. 638-640en_HK
dc.subject.meshAntigens, Viral - blooden_HK
dc.subject.meshCytomegalovirus Infections - diagnosisen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshHemolysisen_HK
dc.subject.meshLeukocytes - immunology - virologyen_HK
dc.titleRapid cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence stainingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=36&issue=3&spage=638&epage=640&date=1998&atitle=Rapid+cytomegalovirus+pp65+antigenemia+assay+by+direct+erythrocyte+lysis+and+immunofluorescence+stainingen_HK
dc.identifier.emailChan, TM:dtmchan@hku.hken_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9508287-
dc.identifier.pmcidPMC104600-
dc.identifier.scopuseid_2-s2.0-0031914931en_HK
dc.identifier.hkuros31532-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031914931&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue3en_HK
dc.identifier.spage638en_HK
dc.identifier.epage640en_HK
dc.identifier.isiWOS:000072035100007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, SKN=36839065300en_HK
dc.identifier.scopusauthoridLo, CY=7401771743en_HK
dc.identifier.scopusauthoridCheng, IKP=7102537483en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK

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