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Article: Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA

TitlePost-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA
Authors
KeywordsSpecies Index: Amphibia
Animalia
Fungi
Mammalia
Reptilia
Vertebrata
Xenopus Laevis
Issue Date2002
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2002, v. 30 n. 7, p. 1664-1669 How to Cite?
AbstractDouble-stranded RNA (dsRNA) induces gene-specific silencing in organisms from fungi to animals, a phenomenon known as RNA interference (RNAi). RNAi represents an evolutionarily conserved system to protect against aberrant expression of genes and a powerful tool for gene manipulation. Despite reports that RNAi can be induced in vertebrates, severe sequence-non-specific effects of long dsRNA have been documented in various systems. It has recently been shown in cultured mammalian cells that small interfering RNAs (siRNAs) of 21-23 nt can mediate RNAi but bypass the non-specific response induced by longer dsRNAs. However, the effectiveness of siRNAs has not been demonstrated in living vertebrates. In addition, the mechanism of siRNA suppression of gene expression in vertebrate cells remains to be elucidated. Here we show that synthetic 21 nt siRNAs can specifically inhibit the expression of exogenously introduced as well as endogenous genes in the embryos of Xenopus laevis. siRNAs significantly reduced the steady-state amount of both the mRNA and protein of the cognate gene target. Moreover, co-injection of siRNA with the target RNA transcript specifically suppressed the activity of the latter. Taken together, our findings establish siRNA-mediated post-transcriptional suppression of gene expression in Xenopus embryos.
Persistent Identifierhttp://hdl.handle.net/10722/48979
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorKok, KHen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2008-06-12T06:31:16Z-
dc.date.available2008-06-12T06:31:16Z-
dc.date.issued2002en_HK
dc.identifier.citationNucleic Acids Research, 2002, v. 30 n. 7, p. 1664-1669en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48979-
dc.description.abstractDouble-stranded RNA (dsRNA) induces gene-specific silencing in organisms from fungi to animals, a phenomenon known as RNA interference (RNAi). RNAi represents an evolutionarily conserved system to protect against aberrant expression of genes and a powerful tool for gene manipulation. Despite reports that RNAi can be induced in vertebrates, severe sequence-non-specific effects of long dsRNA have been documented in various systems. It has recently been shown in cultured mammalian cells that small interfering RNAs (siRNAs) of 21-23 nt can mediate RNAi but bypass the non-specific response induced by longer dsRNAs. However, the effectiveness of siRNAs has not been demonstrated in living vertebrates. In addition, the mechanism of siRNA suppression of gene expression in vertebrate cells remains to be elucidated. Here we show that synthetic 21 nt siRNAs can specifically inhibit the expression of exogenously introduced as well as endogenous genes in the embryos of Xenopus laevis. siRNAs significantly reduced the steady-state amount of both the mRNA and protein of the cognate gene target. Moreover, co-injection of siRNA with the target RNA transcript specifically suppressed the activity of the latter. Taken together, our findings establish siRNA-mediated post-transcriptional suppression of gene expression in Xenopus embryos.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectSpecies Index: Amphibia-
dc.subjectAnimalia-
dc.subjectFungi-
dc.subjectMammalia-
dc.subjectReptilia-
dc.subjectVertebrata-
dc.subjectXenopus Laevis-
dc.subject.meshEmbryo, Nonmammalian - drug effects - metabolismen_HK
dc.subject.meshOligoribonucleotides - administration & dosageen_HK
dc.subject.meshRNA, Double-Stranded - administration & dosageen_HK
dc.subject.meshRecombinant Fusion Proteins - administration & dosage - drug effects - metabolismen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titlePost-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNAen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1048&volume=30&issue=7&spage=1664&epage=1669&date=2002&atitle=Post-transcriptional+suppression+of+gene+expression+in+Xenopus+embryos+by+small+interfering+RNAen_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailKok, KH:khkok@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityKok, KH=rp01455en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/nar/30.7.1664en_HK
dc.identifier.pmid11917028-
dc.identifier.pmcidPMC101847en_HK
dc.identifier.scopuseid_2-s2.0-0036529570en_HK
dc.identifier.hkuros69107-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036529570&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1664en_HK
dc.identifier.epage1669en_HK
dc.identifier.isiWOS:000174654200023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhou, Y=7405366890en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridKok, KH=7006862631en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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