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Article: Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma

TitlePromoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma
Authors
Issue Date2004
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://jmd.amjpathol.org
Citation
Journal Of Molecular Diagnostics, 2004, v. 6 n. 4, p. 326-334 How to Cite?
AbstractThe methylation status of genes in hydatidiform mole and choriocarcinoma and its significance is relatively unexplored. We investigated the methylation status of the promoter regions of six genes, p16, HIC-1, TIMP3, GSTP1, death-associated protein kinase (DAPK), and E-cadherin in 54 hydatidiform moles, five choriocarcinomas, and 10 first trimester placenta by methylation-specific polymerase chain reaction (PCR). Immunohistochemical expression of p16, TIMP3, and E-cadherin, and quantitative real-time RT-PCR of p16 was also performed. Among the six genes examined, the promoter region of four genes (E-cadherin, HIC-1, p16, TIMP3) in choriocarcinoma and three genes (E-cadherin, HIC-1, p16) in hydatidiform mole exhibited aberrant methylation whereas none was hypermethylated in normal placenta. There was a significant correlation between methylation and reduced expression of p16, E-cadherin, and TIMP3 (P < 0.001). Fifteen of the 54 patients with hydatidiform mole developed gestational trophoblastic neoplasia requiring chemotherapy. Promoter hypermethylation of p16 alone, or combined with E-cadherin, was significantly correlated to such development (P = 0.001, 0.0005, respectively). Hypermethylation of multiple genes, especially p16, might be related to the subsequent development of gestational trophoblastic neoplasia. Copyright © American Society for Investigative Pathology. and the Association for Molecular Pathology.
Persistent Identifierhttp://hdl.handle.net/10722/48946
ISSN
2015 Impact Factor: 5.201
2015 SCImago Journal Rankings: 2.514
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXue, WCen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorFeng, HCen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2008-06-12T06:30:24Z-
dc.date.available2008-06-12T06:30:24Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Molecular Diagnostics, 2004, v. 6 n. 4, p. 326-334en_HK
dc.identifier.issn1525-1578en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48946-
dc.description.abstractThe methylation status of genes in hydatidiform mole and choriocarcinoma and its significance is relatively unexplored. We investigated the methylation status of the promoter regions of six genes, p16, HIC-1, TIMP3, GSTP1, death-associated protein kinase (DAPK), and E-cadherin in 54 hydatidiform moles, five choriocarcinomas, and 10 first trimester placenta by methylation-specific polymerase chain reaction (PCR). Immunohistochemical expression of p16, TIMP3, and E-cadherin, and quantitative real-time RT-PCR of p16 was also performed. Among the six genes examined, the promoter region of four genes (E-cadherin, HIC-1, p16, TIMP3) in choriocarcinoma and three genes (E-cadherin, HIC-1, p16) in hydatidiform mole exhibited aberrant methylation whereas none was hypermethylated in normal placenta. There was a significant correlation between methylation and reduced expression of p16, E-cadherin, and TIMP3 (P < 0.001). Fifteen of the 54 patients with hydatidiform mole developed gestational trophoblastic neoplasia requiring chemotherapy. Promoter hypermethylation of p16 alone, or combined with E-cadherin, was significantly correlated to such development (P = 0.001, 0.0005, respectively). Hypermethylation of multiple genes, especially p16, might be related to the subsequent development of gestational trophoblastic neoplasia. Copyright © American Society for Investigative Pathology. and the Association for Molecular Pathology.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://jmd.amjpathol.orgen_HK
dc.relation.ispartofJournal of Molecular Diagnosticsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChoriocarcinoma - geneticsen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshHydatidiform Mole - geneticsen_HK
dc.subject.meshPromoter Regions (Genetics)en_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.titlePromoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1525-1578&volume=6&issue=4&spage=326&epage=334&date=2004&atitle=Promoter+hypermethylation+of+multiple+genes+in+hydatidiform+mole+and+choriocarcinomaen_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid15507671-
dc.identifier.pmcidPMC1867494en_HK
dc.identifier.scopuseid_2-s2.0-8744242129en_HK
dc.identifier.hkuros102378-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-8744242129&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue4en_HK
dc.identifier.spage326en_HK
dc.identifier.epage334en_HK
dc.identifier.isiWOS:000226190000007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridFeng, HC=7401736336en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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