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Article: Application of hepatitis B virus (HBV) DNA sequence polymorphisms to the study of HBV transmission

TitleApplication of hepatitis B virus (HBV) DNA sequence polymorphisms to the study of HBV transmission
Authors
Issue Date1991
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
Journal Of Infectious Diseases, 1991, v. 164 n. 2, p. 284-288 How to Cite?
AbstractShort sequences in hypervariable regions of the hepatitis B virus (HBV) genome can be used to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551-2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this new approach proves the occurrence of intrafamilial transmission of HBV among Chinese.
Persistent Identifierhttp://hdl.handle.net/10722/44980
ISSN
2015 Impact Factor: 6.344
2015 SCImago Journal Rankings: 4.000
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, HJen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorLauder, IJen_HK
dc.contributor.authorWu, PCen_HK
dc.contributor.authorLau, TKen_HK
dc.contributor.authorFong, MWen_HK
dc.date.accessioned2007-10-30T06:14:54Z-
dc.date.available2007-10-30T06:14:54Z-
dc.date.issued1991en_HK
dc.identifier.citationJournal Of Infectious Diseases, 1991, v. 164 n. 2, p. 284-288en_HK
dc.identifier.issn0022-1899en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44980-
dc.description.abstractShort sequences in hypervariable regions of the hepatitis B virus (HBV) genome can be used to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551-2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this new approach proves the occurrence of intrafamilial transmission of HBV among Chinese.en_HK
dc.format.extent936072 bytes-
dc.format.extent1804 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org en_HK
dc.relation.ispartofJournal of Infectious Diseasesen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Infectious Diseases. Copyright © University of Chicago Press.en_HK
dc.subject.meshDNA,-Viral-chemistryen_HK
dc.subject.meshHepatitis-B-transmissionen_HK
dc.subject.meshHepatitis-B-virus-geneticsen_HK
dc.subject.meshPolymorphism,-Geneticen_HK
dc.titleApplication of hepatitis B virus (HBV) DNA sequence polymorphisms to the study of HBV transmissionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1899&volume=164&issue=2&spage=284&epage=288&date=1991&atitle=Application+of+hepatitis+B+virus+(HBV)+DNA+sequence+polymorphisms+to+the+study+of+HBV+transmissionen_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid1856476-
dc.identifier.scopuseid_2-s2.0-0025739283en_HK
dc.identifier.volume164en_HK
dc.identifier.issue2en_HK
dc.identifier.spage284en_HK
dc.identifier.epage288en_HK
dc.identifier.isiWOS:A1991FY10000008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLin, HJ=7405568624en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridLauder, IJ=35564928000en_HK
dc.identifier.scopusauthoridWu, PC=7403119323en_HK
dc.identifier.scopusauthoridLau, TK=35979170400en_HK
dc.identifier.scopusauthoridFong, MW=7005097635en_HK

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