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Article: Abnormal expression and mutation of p53 in cervical cancer - A study at protein, RNA and DNA levels

TitleAbnormal expression and mutation of p53 in cervical cancer - A study at protein, RNA and DNA levels
Authors
KeywordsCervical cancer
HPV
Mutation
p53
RNA
Issue Date1997
PublisherB M J Publishing Group. The Journal's web site is located at http://sti.bmjjournals.com/
Citation
Genitourinary Medicine, 1997, v. 73 n. 1, p. 54-58 How to Cite?
AbstractObjectives: The objectives of this study are to document the status of p53 expression and mutation in cervical cancer at protein, RNA and DNA-levels and to relate this to the presence of HPV. Materials and methods: Biopsy specimens from one hundred and three squamous cell carcinoma of the cervix and histologically normal ectocervix were analysed. Fresh tissues were extracted for protein, RNA and DNA and flash frozen tissue cryostat sectioned for immunohistochemical staining. HPV DNA status was determined by PCR using L1 consensus primers and typed for HPV 16 and 18 with E6 specific primers. p53 expression was determined at the protein level by Western blotting on protein extracts and at RNA level by Northern blotting. Results: There was no p53 overexpression or mutation detectable in the protein extracts. Three of 65 (4.6%) of the carcinomas were positive for p53 by immunostaining with the polyclonal antibody CM1. Overexpression at the RNA level was detected in 2 of 32 (63%) carcinomas. p53 mutation was screened for by PCR/SSCP (single strand conformation polymorphism) followed by sequencing to define the site of mutation. Two of the cervical cancers (2.0%) showed mutation in p53 in exons 7 or 8. The mutation rate in HPV positive rumours was 1.2% (1/81) and in HPV negative tumours was 52% (1/19). Conclusion: p52 overexpression or mutation does not seem to play a significant role in cervical carcinomas.
Persistent Identifierhttp://hdl.handle.net/10722/44571
ISSN
1999 Impact Factor: 1.776
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorStanley, Men_HK
dc.date.accessioned2007-10-30T06:04:36Z-
dc.date.available2007-10-30T06:04:36Z-
dc.date.issued1997en_HK
dc.identifier.citationGenitourinary Medicine, 1997, v. 73 n. 1, p. 54-58en_HK
dc.identifier.issn0266-4348en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44571-
dc.description.abstractObjectives: The objectives of this study are to document the status of p53 expression and mutation in cervical cancer at protein, RNA and DNA-levels and to relate this to the presence of HPV. Materials and methods: Biopsy specimens from one hundred and three squamous cell carcinoma of the cervix and histologically normal ectocervix were analysed. Fresh tissues were extracted for protein, RNA and DNA and flash frozen tissue cryostat sectioned for immunohistochemical staining. HPV DNA status was determined by PCR using L1 consensus primers and typed for HPV 16 and 18 with E6 specific primers. p53 expression was determined at the protein level by Western blotting on protein extracts and at RNA level by Northern blotting. Results: There was no p53 overexpression or mutation detectable in the protein extracts. Three of 65 (4.6%) of the carcinomas were positive for p53 by immunostaining with the polyclonal antibody CM1. Overexpression at the RNA level was detected in 2 of 32 (63%) carcinomas. p53 mutation was screened for by PCR/SSCP (single strand conformation polymorphism) followed by sequencing to define the site of mutation. Two of the cervical cancers (2.0%) showed mutation in p53 in exons 7 or 8. The mutation rate in HPV positive rumours was 1.2% (1/81) and in HPV negative tumours was 52% (1/19). Conclusion: p52 overexpression or mutation does not seem to play a significant role in cervical carcinomas.en_HK
dc.format.extent480565 bytes-
dc.format.extent3653 bytes-
dc.format.extent3017 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://sti.bmjjournals.com/en_HK
dc.relation.ispartofGenitourinary Medicineen_HK
dc.rightsGenitourinary Medicine. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCervical canceren_HK
dc.subjectHPVen_HK
dc.subjectMutationen_HK
dc.subjectp53en_HK
dc.subjectRNAen_HK
dc.subject.meshCarcinoma,-Squamous-Cell-metabolismen_HK
dc.subject.meshGenes,-p53-geneticsen_HK
dc.subject.meshMutation-en_HK
dc.subject.meshTumor-Suppressor-Protein-p53-geneticsen_HK
dc.subject.meshTumor-Suppressor-Protein-p53-metabolismen_HK
dc.titleAbnormal expression and mutation of p53 in cervical cancer - A study at protein, RNA and DNA levelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0266-4348&volume=73&issue=1&spage=54&epage=58&date=1997&atitle=Abnormal+expression+and+mutation+of+p53+in+cervical+cancer--a+study+at+protein,+RNA+and+DNA+levelsen_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/sti.73.1.54-
dc.identifier.pmid9155557en_HK
dc.identifier.pmcidPMC1195761-
dc.identifier.scopuseid_2-s2.0-79960451862en_HK
dc.identifier.hkuros21791-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031050020&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue1en_HK
dc.identifier.spage54en_HK
dc.identifier.epage58en_HK
dc.identifier.isiWOS:A1997WJ75000013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridStanley, M=7202497085en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100-
dc.identifier.scopusauthoridTsao, SW=7102813116-
dc.identifier.scopusauthoridLiu, SS=37102450400-
dc.identifier.scopusauthoridStanley, M=7202497085-

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