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Article: Caspase activity is downregulated in choriocarcinoma: A cDNA array differential expression study
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TitleCaspase activity is downregulated in choriocarcinoma: A cDNA array differential expression study
 
AuthorsFong, PY1
Xue, WC1
Ngan, HYS1
Chiu, PM1
Chan, KYK1
Tsao, SW1
Cheung, ANY1
 
Issue Date2006
 
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
CitationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 179-183 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2005.028027
 
AbstractBakground: Placental trophoblast can be considered to be pseudomalignant tissue and the pathogenesis of gestational trophoblastic diseases remains to be clarified. Aims: To examine the role of caspases 8 and 10, identified by differential expression, on trophoblast tumorigenesis. Methods: cDNA array hybridisation was used to compare gene expression profiles in choriocarcinoma cell lines (JAR, JEG, and BeWo) and normal first trimester human placentas, followed by confirmation with quantitative real time polymerase chain reaction and immunohistochemistry. Caspase 10 and its closely related family member caspase 8 were analysed. Results: Downregulation of caspase 10 in choriocarcinoma was detected by both Atlas™ human cDNA expression array and Atlas™ human 1.2 array. Caspase 10 mRNA expression was significantly lower in hydatidiform mole (p = 0.035) and chorioarcinoma (p = 0.002) compared with normal placenta. The caspase 8 and 10 proteins were expressed predominantly in the cytotrophoblast and syncytiotrophoblast, respectively, with significantly lower expression in choriocarcinomas than other trophoblastic tissues (p < 0.05). Immunoreactivity for both caspase 8 and 10 correlated with the apoptotic index previously assessed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (p = 0.02 and p = 0.04, respectively) and M30 (p < 0.001 and p = 0.003, respectively) approaches. Conclusions: These results suggest that the downregulation of capases 8 and 10 might contribute to the pathogenesis of choriocarcinoma.
 
ISSN0021-9746
2012 Impact Factor: 2.439
2012 SCImago Journal Rankings: 0.980
 
DOIhttp://dx.doi.org/10.1136/jcp.2005.028027
 
PubMed Central IDPMC1860314
 
ISI Accession Number IDWOS:000234938100012
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFong, PY
 
dc.contributor.authorXue, WC
 
dc.contributor.authorNgan, HYS
 
dc.contributor.authorChiu, PM
 
dc.contributor.authorChan, KYK
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorCheung, ANY
 
dc.date.accessioned2007-10-30T06:04:32Z
 
dc.date.available2007-10-30T06:04:32Z
 
dc.date.issued2006
 
dc.description.abstractBakground: Placental trophoblast can be considered to be pseudomalignant tissue and the pathogenesis of gestational trophoblastic diseases remains to be clarified. Aims: To examine the role of caspases 8 and 10, identified by differential expression, on trophoblast tumorigenesis. Methods: cDNA array hybridisation was used to compare gene expression profiles in choriocarcinoma cell lines (JAR, JEG, and BeWo) and normal first trimester human placentas, followed by confirmation with quantitative real time polymerase chain reaction and immunohistochemistry. Caspase 10 and its closely related family member caspase 8 were analysed. Results: Downregulation of caspase 10 in choriocarcinoma was detected by both Atlas™ human cDNA expression array and Atlas™ human 1.2 array. Caspase 10 mRNA expression was significantly lower in hydatidiform mole (p = 0.035) and chorioarcinoma (p = 0.002) compared with normal placenta. The caspase 8 and 10 proteins were expressed predominantly in the cytotrophoblast and syncytiotrophoblast, respectively, with significantly lower expression in choriocarcinomas than other trophoblastic tissues (p < 0.05). Immunoreactivity for both caspase 8 and 10 correlated with the apoptotic index previously assessed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (p = 0.02 and p = 0.04, respectively) and M30 (p < 0.001 and p = 0.003, respectively) approaches. Conclusions: These results suggest that the downregulation of capases 8 and 10 might contribute to the pathogenesis of choriocarcinoma.
 
dc.description.naturepublished_or_final_version
 
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dc.identifier.citationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 179-183 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2005.028027
 
dc.identifier.doihttp://dx.doi.org/10.1136/jcp.2005.028027
 
dc.identifier.epage183
 
dc.identifier.isiWOS:000234938100012
 
dc.identifier.issn0021-9746
2012 Impact Factor: 2.439
2012 SCImago Journal Rankings: 0.980
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC1860314
 
dc.identifier.pmid16443735
 
dc.identifier.scopuseid_2-s2.0-32544455999
 
dc.identifier.spage179
 
dc.identifier.urihttp://hdl.handle.net/10722/44568
 
dc.identifier.volume59
 
dc.languageeng
 
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Clinical Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCaspases-biosynthesis
 
dc.subject.meshChoriocarcinoma-enzymology
 
dc.subject.meshGene-Expression-Regulation,-Neoplastic
 
dc.subject.meshUterine-Neoplasms-enzymology
 
dc.titleCaspase activity is downregulated in choriocarcinoma: A cDNA array differential expression study
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong