Article: Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex

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TitleSpecific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex
AuthorsChing, YP2
Chun, ACS2
Chin, KT2
Zhang, ZQ1
Jeang, KT
Jin, DY3
Issue Date2004
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.retrovirology.com/home/
CitationRetrovirology, 2004, v. 1 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1742-4690-1-18
AbstractBackground: Human T-cell leukemia virus type I (HTLV-I) Tax protein is a transcriptional regulator of viral and cellular genes. In this study we have examined in detail the determinants for Tax-mediated transcriptional activation. Results: Whereas previously the LTR enhancer elements were thought to be the sole Tax-targets, herein, we find that the core HTLV-I TATAA motif also provides specific responsiveness not seen with either the SV40 or the E1b TATAA boxes. When enhancer elements which can mediate Tax-responsiveness were compared, the authentic HTLV-I 21-bp repeats were found to be the most effective. Related bZIP factors such as CREB, ATF4, c-Jun and LZIP are often thought to recognize the 21-bp repeats equivalently. However, amongst bZIP factors, we found that CREB, by far, is preferred by Tax for activation. When LTR transcription was reconstituted by substituting either κB or serum response elements in place of the 21-bp repeats, Tax activated these surrogate motifs using surfaces which are different from that utilized for CREB interaction. Finally, we employed artificial recruitment of TATA-binding protein to the HTLV-I promoter in "bypass" experiments to show for the first time that Tax has transcriptional activity subsequent to the assembly of an initiation complex at the promoter. Conclusions: Optimal activation of the HTLV-I LTR by Tax specifically requires the core HTLV-I TATAA promoter, CREB and the 21-bp repeats. In addition, we also provide the first evidence for transcriptional activity of Tax after the recruitment of TATA-binding protein to the promoter. © 2004 Ching et al; licensee BioMed Central Ltd.
ISSN1742-4690
2011 Impact Factor: 6.47
2011 SCImago Journal Rankings: 0.751
DOIhttp://dx.doi.org/10.1186/1742-4690-1-18
PubMed Central IDPMC509288
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChing, YP
dc.contributor.authorChun, ACS
dc.contributor.authorChin, KT
dc.contributor.authorZhang, ZQ
dc.contributor.authorJeang, KT
dc.contributor.authorJin, DY
dc.date.accessioned2007-03-23T04:28:01Z
dc.date.available2007-03-23T04:28:01Z
dc.date.issued2004
dc.description.abstractBackground: Human T-cell leukemia virus type I (HTLV-I) Tax protein is a transcriptional regulator of viral and cellular genes. In this study we have examined in detail the determinants for Tax-mediated transcriptional activation. Results: Whereas previously the LTR enhancer elements were thought to be the sole Tax-targets, herein, we find that the core HTLV-I TATAA motif also provides specific responsiveness not seen with either the SV40 or the E1b TATAA boxes. When enhancer elements which can mediate Tax-responsiveness were compared, the authentic HTLV-I 21-bp repeats were found to be the most effective. Related bZIP factors such as CREB, ATF4, c-Jun and LZIP are often thought to recognize the 21-bp repeats equivalently. However, amongst bZIP factors, we found that CREB, by far, is preferred by Tax for activation. When LTR transcription was reconstituted by substituting either κB or serum response elements in place of the 21-bp repeats, Tax activated these surrogate motifs using surfaces which are different from that utilized for CREB interaction. Finally, we employed artificial recruitment of TATA-binding protein to the HTLV-I promoter in "bypass" experiments to show for the first time that Tax has transcriptional activity subsequent to the assembly of an initiation complex at the promoter. Conclusions: Optimal activation of the HTLV-I LTR by Tax specifically requires the core HTLV-I TATAA promoter, CREB and the 21-bp repeats. In addition, we also provide the first evidence for transcriptional activity of Tax after the recruitment of TATA-binding protein to the promoter. © 2004 Ching et al; licensee BioMed Central Ltd.
dc.description.naturepublished_or_final_version
dc.format.extent1175820 bytes
dc.format.extent25088 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/msword
dc.identifier.citationRetrovirology, 2004, v. 1 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1742-4690-1-18
dc.identifier.doihttp://dx.doi.org/10.1186/1742-4690-1-18
dc.identifier.hkuros94878
dc.identifier.issn1742-4690
2011 Impact Factor: 6.47
2011 SCImago Journal Rankings: 0.751
dc.identifier.openurl
dc.identifier.pmcidPMC509288
dc.identifier.pmid15285791
dc.identifier.scopuseid_2-s2.0-17744377229
dc.identifier.urihttp://hdl.handle.net/10722/42620
dc.identifier.volume1
dc.languageeng
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.retrovirology.com/home/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofRetrovirology
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshAntigens, neoplasm - genetics
dc.subject.meshGene products, tax - metabolism
dc.subject.meshHistocompatibility antigens - genetics
dc.subject.meshHuman t-lymphotropic virus 1 - genetics
dc.subject.meshPeptide chain initiation, translational
dc.titleSpecific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex
dc.typeArticle
Author Affiliations
  1. National Laboratory of Medical Molecular Biology Beijing
  2. The University of Hong Kong
  3. National Institutes of Health, Bethesda