Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jcmgh.2020.09.017
- Scopus: eid_2-s2.0-85100051853
- PMID: 33010495
- WOS: WOS:000621254400007
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: SARS-CoV-2 induces a more robust innate immune response and replicates less efficiently than SARS-CoV in the human intestines: An ex vivo study with implications on pathogenesis of COVID-19
Title | SARS-CoV-2 induces a more robust innate immune response and replicates less efficiently than SARS-CoV in the human intestines: An ex vivo study with implications on pathogenesis of COVID-19 |
---|---|
Authors | |
Keywords | COVID-19 Immune Activation Intestine Replication SARS-CoV |
Issue Date | 2021 |
Publisher | Elsevier: Open Access Journals. The Journal's web site is located at https://www.cmghjournal.org/ |
Citation | Cellular and Molecular Gastroenterology and Hepatology, 2021, v. 11 n. 3, p. 771-781 How to Cite? |
Abstract | Background and Aims:
Besides prominent respiratory involvement, gastrointestinal manifestations are commonly reported in Coronavirus Disease 2019 (COVID-19) patients. We compared infection of ex vivo human intestinal tissues by SARS-CoV-2 and SARS-CoV with respect to their replication kinetics and immune activation profile.
Methods:
Human intestinal tissues were obtained from patients while undergoing surgical operations at Queen Mary Hospital, Hong Kong. Upon surgical removal, the tissues were immediately processed and infected with SARS-CoV-2 or SARS-CoV. Replication kinetics were determined with immunohistochemistry, qRT-PCR, and plaque assays. Immune activation in the infected intestinal tissues was assessed by detecting the gene expression of interferons and representative pro-inflammatory cytokines and chemokines.
Results:
SARS-CoV-2 could infect and productively replicate in the ex vivo human intestinal tissues with release of infectious virus particles, but not in ex vivo human liver and kidney tissues. Importantly, SARS-CoV-2 replicated less efficiently than SARS-CoV, induced less cytopathology in the human intestinal epithelium, and induced a more robust innate immune response including the activation of both type I and type III interferons, than SARS-CoV in human intestinal tissues.
Conclusion:
Using the ex vivo human intestinal tissues as a physiologically relevant model, our data indicated that SARS-CoV-2 could productively replicate in the human gut and suggested that the gastrointestinal tract might serve as an alternative route of virus dissemination. SARS-CoV-2 replicated less efficiently and induced less cytopathology than SARS-CoV in keeping with the clinical observations reported for COVID-19 and SARS, which might be the result of a more robust immune activation by SARS-CoV-2 than SARS-CoV in the human intestine. |
Persistent Identifier | http://hdl.handle.net/10722/298771 |
ISSN | 2023 Impact Factor: 7.1 2023 SCImago Journal Rankings: 2.702 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chu, H | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Wang, Y | - |
dc.contributor.author | Yuen, TTT | - |
dc.contributor.author | Chai, Y | - |
dc.contributor.author | Shuai, H | - |
dc.contributor.author | YANG, D | - |
dc.contributor.author | Hu, B | - |
dc.contributor.author | Huang, X | - |
dc.contributor.author | Zhang, X | - |
dc.contributor.author | Hou, Y | - |
dc.contributor.author | Cai, JP | - |
dc.contributor.author | Zhang, J | - |
dc.contributor.author | Zhou, J | - |
dc.contributor.author | Yuan, S | - |
dc.contributor.author | To, KKW | - |
dc.contributor.author | Hung, IFN | - |
dc.contributor.author | Cheung, TT | - |
dc.contributor.author | Ng, ATL | - |
dc.contributor.author | Chan, HYI | - |
dc.contributor.author | Wong, IYH | - |
dc.contributor.author | Law, SYK | - |
dc.contributor.author | Foo, DCC | - |
dc.contributor.author | Leung, WK | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2021-04-12T03:03:08Z | - |
dc.date.available | 2021-04-12T03:03:08Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Cellular and Molecular Gastroenterology and Hepatology, 2021, v. 11 n. 3, p. 771-781 | - |
dc.identifier.issn | 2352-345X | - |
dc.identifier.uri | http://hdl.handle.net/10722/298771 | - |
dc.description.abstract | Background and Aims: Besides prominent respiratory involvement, gastrointestinal manifestations are commonly reported in Coronavirus Disease 2019 (COVID-19) patients. We compared infection of ex vivo human intestinal tissues by SARS-CoV-2 and SARS-CoV with respect to their replication kinetics and immune activation profile. Methods: Human intestinal tissues were obtained from patients while undergoing surgical operations at Queen Mary Hospital, Hong Kong. Upon surgical removal, the tissues were immediately processed and infected with SARS-CoV-2 or SARS-CoV. Replication kinetics were determined with immunohistochemistry, qRT-PCR, and plaque assays. Immune activation in the infected intestinal tissues was assessed by detecting the gene expression of interferons and representative pro-inflammatory cytokines and chemokines. Results: SARS-CoV-2 could infect and productively replicate in the ex vivo human intestinal tissues with release of infectious virus particles, but not in ex vivo human liver and kidney tissues. Importantly, SARS-CoV-2 replicated less efficiently than SARS-CoV, induced less cytopathology in the human intestinal epithelium, and induced a more robust innate immune response including the activation of both type I and type III interferons, than SARS-CoV in human intestinal tissues. Conclusion: Using the ex vivo human intestinal tissues as a physiologically relevant model, our data indicated that SARS-CoV-2 could productively replicate in the human gut and suggested that the gastrointestinal tract might serve as an alternative route of virus dissemination. SARS-CoV-2 replicated less efficiently and induced less cytopathology than SARS-CoV in keeping with the clinical observations reported for COVID-19 and SARS, which might be the result of a more robust immune activation by SARS-CoV-2 than SARS-CoV in the human intestine. | - |
dc.language | eng | - |
dc.publisher | Elsevier: Open Access Journals. The Journal's web site is located at https://www.cmghjournal.org/ | - |
dc.relation.ispartof | Cellular and Molecular Gastroenterology and Hepatology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | COVID-19 | - |
dc.subject | Immune Activation | - |
dc.subject | Intestine | - |
dc.subject | Replication | - |
dc.subject | SARS-CoV | - |
dc.title | SARS-CoV-2 induces a more robust innate immune response and replicates less efficiently than SARS-CoV in the human intestines: An ex vivo study with implications on pathogenesis of COVID-19 | - |
dc.type | Article | - |
dc.identifier.email | Chu, H: hinchu@hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Shuai, H: shuaihp@connect.hku.hk | - |
dc.identifier.email | Hu, B: bingjie@hku.hk | - |
dc.identifier.email | Cai, JP: caijuice@hku.hk | - |
dc.identifier.email | Zhang, J: zhangajx@hkucc.hku.hk | - |
dc.identifier.email | Zhou, J: jiezhou@hku.hk | - |
dc.identifier.email | Yuan, S: yuansf@hku.hk | - |
dc.identifier.email | To, KKW: kelvinto@hku.hk | - |
dc.identifier.email | Hung, IFN: ivanhung@hkucc.hku.hk | - |
dc.identifier.email | Cheung, TT: cheung68@hku.hk | - |
dc.identifier.email | Ng, ATL: ada5022@hku.hk | - |
dc.identifier.email | Chan, HYI: ivyhchan@hku.hk | - |
dc.identifier.email | Wong, IYH: iyhwong@hku.hk | - |
dc.identifier.email | Law, SYK: slaw@hku.hk | - |
dc.identifier.email | Foo, DCC: ccfoo@hku.hk | - |
dc.identifier.email | Leung, WK: waikleung@hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Chu, H=rp02125 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Zhang, J=rp00413 | - |
dc.identifier.authority | Zhou, J=rp01412 | - |
dc.identifier.authority | Yuan, S=rp02640 | - |
dc.identifier.authority | To, KKW=rp01384 | - |
dc.identifier.authority | Hung, IFN=rp00508 | - |
dc.identifier.authority | Cheung, TT=rp02129 | - |
dc.identifier.authority | Wong, IYH=rp02293 | - |
dc.identifier.authority | Law, SYK=rp00437 | - |
dc.identifier.authority | Foo, DCC=rp01899 | - |
dc.identifier.authority | Leung, WK=rp01479 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.jcmgh.2020.09.017 | - |
dc.identifier.pmid | 33010495 | - |
dc.identifier.pmcid | PMC7527315 | - |
dc.identifier.scopus | eid_2-s2.0-85100051853 | - |
dc.identifier.hkuros | 322084 | - |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 771 | - |
dc.identifier.epage | 781 | - |
dc.identifier.isi | WOS:000621254400007 | - |
dc.publisher.place | United States | - |