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Article: A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets

TitleA Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets
Authors
Keywordscombinatorial genetics
CombiGEM
CRISPR
combination therapy
high-throughput screening
Issue Date2020
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports
Citation
Cell Reports, 2020, v. 32 n. 6, p. article no. 108020 How to Cite?
AbstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.
Persistent Identifierhttp://hdl.handle.net/10722/287249
ISSN
2019 Impact Factor: 8.109
2015 SCImago Journal Rankings: 8.588

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorChan, BKC-
dc.contributor.authorWan, YK-
dc.contributor.authorYUEN, CTL-
dc.contributor.authorChoi, GCG-
dc.contributor.authorLI, X-
dc.contributor.authorTong, CSW-
dc.contributor.authorZhong, SSW-
dc.contributor.authorSun, J-
dc.contributor.authorBao, Y-
dc.contributor.authorMak, SYL-
dc.contributor.authorChow, MZY-
dc.contributor.authorKhaw, JV-
dc.contributor.authorLeung, SY-
dc.contributor.authorZheng, Z-
dc.contributor.authorCheung, LWT-
dc.contributor.authorTan, K-
dc.contributor.authorWong, KH-
dc.contributor.authorChan, HYE-
dc.contributor.authorWong, ASL-
dc.date.accessioned2020-09-22T02:58:07Z-
dc.date.available2020-09-22T02:58:07Z-
dc.date.issued2020-
dc.identifier.citationCell Reports, 2020, v. 32 n. 6, p. article no. 108020-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/287249-
dc.description.abstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports-
dc.relation.ispartofCell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcombinatorial genetics-
dc.subjectCombiGEM-
dc.subjectCRISPR-
dc.subjectcombination therapy-
dc.subjecthigh-throughput screening-
dc.titleA Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets-
dc.typeArticle-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.identifier.emailChan, BKC: beckyckc@hku.hk-
dc.identifier.emailChoi, GCG: gigichoi@hku.hk-
dc.identifier.emailTong, CSW: cswtong@hku.hk-
dc.identifier.emailZhong, SSW: u3536772@connect.hku.hk-
dc.identifier.emailSun, J: sunjr@HKUCC-COM.hku.hk-
dc.identifier.emailLeung, SY: suetyi@hku.hk-
dc.identifier.emailCheung, LWT: lydiacwt@hku.hk-
dc.identifier.emailWong, ASL: aslw@hku.hk-
dc.identifier.authorityLeung, SY=rp00359-
dc.identifier.authorityCheung, LWT=rp02137-
dc.identifier.authorityWong, ASL=rp02139-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.celrep.2020.108020-
dc.identifier.pmid32783942-
dc.identifier.scopuseid_2-s2.0-85089183838-
dc.identifier.hkuros314202-
dc.identifier.volume32-
dc.identifier.issue6-
dc.identifier.spagearticle no. 108020-
dc.identifier.epagearticle no. 108020-
dc.publisher.placeUnited States-

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