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Conference Paper: A Ratiometric Fluorescent Probe for Detection and Quantification of Endogenous Hydrogen Peroxide in Living Cells

TitleA Ratiometric Fluorescent Probe for Detection and Quantification of Endogenous Hydrogen Peroxide in Living Cells
Society for Redox Biology and Medicine (SfRBM) Annual Meeting, 2019
Authors
Issue Date2019
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
The Society for Redox Biology and Medicine (SfRBM) 26th Annual Meeting, Las Vegas, NV, USA, 20-23 November 2019. Abstracts in Free Radical Biology & Medicine, 2019, v. 145 n. Suppl. 1, p. S43, abstract no. 73 How to Cite?
AbstractHKPerox-ratio, a new type of ratiometric fluorescent probe, is developed for cellular hydrogen peroxide (H2O2) detection and quantification employing a H2O2-mediated tandem Payne/Dakin reaction. HKPerox-ratio exhibited a 65 nm shift from blue emission (Fblue) to green emission (Fgreen) upon H2O2 treatment, and the fluorescence ratio Fgreen/Fblue is linearly correlated with H2O2 concentrations. H2O2 burst induced by arsenic trioxide was successfully detected by HKPerox-ratio in multiple leukemia cell types including THP-1, NB4, and U937 cells. In addition, endogenous H2O2 production in living cells in response to starvation has also been visualized and quantified by HKPerox-ratio with both confocal microscopy and flow cytometry. This study provides a novel and reliable molecular tool for cellular H2O2 quantifications in redox biology research.
DescriptionPoster Presentation - Poster No. 073
Persistent Identifierhttp://hdl.handle.net/10722/284170
ISSN
2019 Impact Factor: 6.17
2015 SCImago Journal Rankings: 2.468
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYe, S-
dc.contributor.authorHu, J-
dc.contributor.authorZhao, Q-
dc.contributor.authorYang, D-
dc.date.accessioned2020-07-20T05:56:38Z-
dc.date.available2020-07-20T05:56:38Z-
dc.date.issued2019-
dc.identifier.citationThe Society for Redox Biology and Medicine (SfRBM) 26th Annual Meeting, Las Vegas, NV, USA, 20-23 November 2019. Abstracts in Free Radical Biology & Medicine, 2019, v. 145 n. Suppl. 1, p. S43, abstract no. 73-
dc.identifier.issn0891-5849-
dc.identifier.urihttp://hdl.handle.net/10722/284170-
dc.descriptionPoster Presentation - Poster No. 073-
dc.description.abstractHKPerox-ratio, a new type of ratiometric fluorescent probe, is developed for cellular hydrogen peroxide (H2O2) detection and quantification employing a H2O2-mediated tandem Payne/Dakin reaction. HKPerox-ratio exhibited a 65 nm shift from blue emission (Fblue) to green emission (Fgreen) upon H2O2 treatment, and the fluorescence ratio Fgreen/Fblue is linearly correlated with H2O2 concentrations. H2O2 burst induced by arsenic trioxide was successfully detected by HKPerox-ratio in multiple leukemia cell types including THP-1, NB4, and U937 cells. In addition, endogenous H2O2 production in living cells in response to starvation has also been visualized and quantified by HKPerox-ratio with both confocal microscopy and flow cytometry. This study provides a novel and reliable molecular tool for cellular H2O2 quantifications in redox biology research.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed-
dc.relation.ispartofFree Radical Biology & Medicine-
dc.titleA Ratiometric Fluorescent Probe for Detection and Quantification of Endogenous Hydrogen Peroxide in Living Cells-
dc.titleSociety for Redox Biology and Medicine (SfRBM) Annual Meeting, 2019-
dc.typeConference_Paper-
dc.identifier.emailYe, S: yesen@hku.hk-
dc.identifier.emailYang, D: yangdan@hku.hk-
dc.identifier.authorityYang, D=rp00825-
dc.identifier.doi10.1016/j.freeradbiomed.2019.10.111-
dc.identifier.hkuros311045-
dc.identifier.volume145-
dc.identifier.issueSuppl. 1-
dc.identifier.spageS43, abstract no. 73-
dc.identifier.epageS43, abstract no. 73-
dc.identifier.isiWOS:000496777400098-
dc.publisher.placeUnited States-
dc.identifier.issnl0891-5849-

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