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Conference Paper: In vivo electroporation-mediated transfer of interleukin-35 gene decreases demyelination in animal model of multiple sclerosis

TitleIn vivo electroporation-mediated transfer of interleukin-35 gene decreases demyelination in animal model of multiple sclerosis
Authors
Issue Date2019
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/
Citation
The 24th Medical Research Conference, Hong Kong, 19 January 2019. In Hong Kong Medical Journal, 2019, ;25 n. 1, Suppl. 1, p. 32, abstract no. 45 How to Cite?
AbstractIntroduction: Multiple sclerosis (MS) is an immune-mediated demyelinating disease, in which T cell tolerance is deteriorated to myelin antigens. Interleukin-35 (IL-35) is a novel anti-inflammatory cytokine, which belongs to the IL-12 family and includes p35 and Epstein-Barr virus (Ebi3). Evidence demonstrates that IL-35 has a potential to ameliorate autoimmune diseases by suppressing inflammatory lymphocytes and inducing regulatory lymphocytes. In this study, we aimed to clarify the IL-35 expression profile in response to experimental autoimmune encephalomyelitis (EAE), a model of MS in mice, and investigate the therapeutic potential of IL-35 by overexpressing it in EAE mice. Methods: Experimental autoimmune encephalomyelitis was induced in C57BL/6 mice by immunisation with MOG35-55 peptide. Mice were sacrificed at 14 days (initiative phase), 21 days (progressive phase), and 28 days (recovery phase) after immunisation. For electrotransfer of IL-35, recombinant murine IL-35 was encoded in pIGneo DNA plasmid and transferred into bilateral tibialis anterior muscles of EAE mice by electroporation at 10 days and 17 days after immunisation. Mice were sacrificed at 21 days after immunisation. Results: Expression of Ebi3 and p35 was downregulated during the progressive phase of EAE and upregulated during the recovery phase. Experimental autoimmune encephalomyelitis severity was attenuated in IL-35–transferred EAE mice. Correspondingly, the fluorescence intensity of myelin basic protein was elevated in IL-35–transferred EAE mice (P<0.001) accompanied with marked decrease of glial fibrillary acidic protein–positive (GFAP+) cells in the white matter of spinal cord from IL-35–transferred EAE mice compared with that from empty vector–transferred EAE mice (P<0.001). However, the fluorescence intensity of ionised calcium-binding adaptor molecule 1 did not show any significant difference. Conclusion: Taken together, these results demonstrate that in troducing IL-35 by electroporation effectively reduces EAE severity and prevents demyelination, which is probably through inhibiting the activation of GFAP+ astrocytes but not through microglia inactivation.
DescriptionOrganizer: Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong
Persistent Identifierhttp://hdl.handle.net/10722/275309
ISSN
2017 Impact Factor: 1.226
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorMa, KFO-
dc.contributor.authorNg, CL-
dc.contributor.authorYick, LW-
dc.contributor.authorJian, M-
dc.contributor.authorChan, KH-
dc.date.accessioned2019-09-10T02:39:55Z-
dc.date.available2019-09-10T02:39:55Z-
dc.date.issued2019-
dc.identifier.citationThe 24th Medical Research Conference, Hong Kong, 19 January 2019. In Hong Kong Medical Journal, 2019, ;25 n. 1, Suppl. 1, p. 32, abstract no. 45-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/275309-
dc.descriptionOrganizer: Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong-
dc.description.abstractIntroduction: Multiple sclerosis (MS) is an immune-mediated demyelinating disease, in which T cell tolerance is deteriorated to myelin antigens. Interleukin-35 (IL-35) is a novel anti-inflammatory cytokine, which belongs to the IL-12 family and includes p35 and Epstein-Barr virus (Ebi3). Evidence demonstrates that IL-35 has a potential to ameliorate autoimmune diseases by suppressing inflammatory lymphocytes and inducing regulatory lymphocytes. In this study, we aimed to clarify the IL-35 expression profile in response to experimental autoimmune encephalomyelitis (EAE), a model of MS in mice, and investigate the therapeutic potential of IL-35 by overexpressing it in EAE mice. Methods: Experimental autoimmune encephalomyelitis was induced in C57BL/6 mice by immunisation with MOG35-55 peptide. Mice were sacrificed at 14 days (initiative phase), 21 days (progressive phase), and 28 days (recovery phase) after immunisation. For electrotransfer of IL-35, recombinant murine IL-35 was encoded in pIGneo DNA plasmid and transferred into bilateral tibialis anterior muscles of EAE mice by electroporation at 10 days and 17 days after immunisation. Mice were sacrificed at 21 days after immunisation. Results: Expression of Ebi3 and p35 was downregulated during the progressive phase of EAE and upregulated during the recovery phase. Experimental autoimmune encephalomyelitis severity was attenuated in IL-35–transferred EAE mice. Correspondingly, the fluorescence intensity of myelin basic protein was elevated in IL-35–transferred EAE mice (P<0.001) accompanied with marked decrease of glial fibrillary acidic protein–positive (GFAP+) cells in the white matter of spinal cord from IL-35–transferred EAE mice compared with that from empty vector–transferred EAE mice (P<0.001). However, the fluorescence intensity of ionised calcium-binding adaptor molecule 1 did not show any significant difference. Conclusion: Taken together, these results demonstrate that in troducing IL-35 by electroporation effectively reduces EAE severity and prevents demyelination, which is probably through inhibiting the activation of GFAP+ astrocytes but not through microglia inactivation.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartofHong Kong Medical Journal-
dc.relation.ispartof24th Medical Research Conference-
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleIn vivo electroporation-mediated transfer of interleukin-35 gene decreases demyelination in animal model of multiple sclerosis-
dc.typeConference_Paper-
dc.identifier.emailMa, KFO: oscarmkf@HKUCC-COM.hku.hk-
dc.identifier.emailNg, CL: royclng@hku.hk-
dc.identifier.emailYick, LW: lwyick@hku.hk-
dc.identifier.emailChan, KH: koonho@hku.hk-
dc.identifier.authorityNg, CL=rp02376-
dc.identifier.authorityChan, KH=rp00537-
dc.identifier.hkuros302695-
dc.identifier.issue1, Suppl. 1-
dc.identifier.spage32, abstract no. 45-
dc.identifier.epage32, abstract no. 45-
dc.publisher.placeHong Kong-

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