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Article: The combined antimicrobial effect of arginine and fluoride toothpaste.
Title | The combined antimicrobial effect of arginine and fluoride toothpaste. |
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Authors | |
Issue Date | 2019 |
Publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html |
Citation | Scientific Reports, 2019, v. 9 n. 1, p. article no. 8405 How to Cite? |
Abstract | The aim of the study was to investigate the antibacterial effect of arginine (Arg) in NaF toothpaste. 24-h mono-/3-species biofilm cultures of S. mutans, S. sanguis and S. gordonii inoculated sHA discs were subjected to treatment with toothpaste supernatants prepared as - [1]:2% Arg –NaF (0.147% F), [2]:4% Arg–NaF (0.144% F), [3]:8% Arg – NaF (0.138% F), [4]:NaF (0.15%) and [5]:deionized water. After 24-h incubation, the mono-species biofilms were subjected to viability assay using WST-8, SEM and confocal imaging (CLSM). The 3-species biofilm were quantified for bacterial composition by PCR analysis, SEM, CLSM, and RNA isolation with reverse-transcription PCR analysis. Increasing arginine concentrations in NaF toothpaste had no effect on microbial viability. The mono-/3-species biofilm imaging depicted that the 2% Arg-NaF and 4% Arg-NaF had a biofilm disrupting effect. The 3-species biofilm bacterial composition indicated that the 2% Arg-NaF group maintained an ecological homeostasis by inhibiting S. mutans growth and enriching the growth of S. sanguis and S. gordonii. The 2% Arg-NaF group significantly downregulated the expression of virulent gtfB gene and upregulated the expression of sagP with relative dominance of arcA. Incorporation of 2% arginine in NaF toothpaste might enrich the alkali-producing bacteria and provide enhanced counter mechanisms against cariogenic pathogen when compared to NaF toothpaste. © 2019, The Author(s). |
Persistent Identifier | http://hdl.handle.net/10722/274811 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.900 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bijle, MNA | - |
dc.contributor.author | Ekambaram, M | - |
dc.contributor.author | Lo, ECM | - |
dc.contributor.author | Yiu, CKY | - |
dc.date.accessioned | 2019-09-10T02:29:16Z | - |
dc.date.available | 2019-09-10T02:29:16Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Scientific Reports, 2019, v. 9 n. 1, p. article no. 8405 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10722/274811 | - |
dc.description.abstract | The aim of the study was to investigate the antibacterial effect of arginine (Arg) in NaF toothpaste. 24-h mono-/3-species biofilm cultures of S. mutans, S. sanguis and S. gordonii inoculated sHA discs were subjected to treatment with toothpaste supernatants prepared as - [1]:2% Arg –NaF (0.147% F), [2]:4% Arg–NaF (0.144% F), [3]:8% Arg – NaF (0.138% F), [4]:NaF (0.15%) and [5]:deionized water. After 24-h incubation, the mono-species biofilms were subjected to viability assay using WST-8, SEM and confocal imaging (CLSM). The 3-species biofilm were quantified for bacterial composition by PCR analysis, SEM, CLSM, and RNA isolation with reverse-transcription PCR analysis. Increasing arginine concentrations in NaF toothpaste had no effect on microbial viability. The mono-/3-species biofilm imaging depicted that the 2% Arg-NaF and 4% Arg-NaF had a biofilm disrupting effect. The 3-species biofilm bacterial composition indicated that the 2% Arg-NaF group maintained an ecological homeostasis by inhibiting S. mutans growth and enriching the growth of S. sanguis and S. gordonii. The 2% Arg-NaF group significantly downregulated the expression of virulent gtfB gene and upregulated the expression of sagP with relative dominance of arcA. Incorporation of 2% arginine in NaF toothpaste might enrich the alkali-producing bacteria and provide enhanced counter mechanisms against cariogenic pathogen when compared to NaF toothpaste. © 2019, The Author(s). | - |
dc.language | eng | - |
dc.publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html | - |
dc.relation.ispartof | Scientific Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | The combined antimicrobial effect of arginine and fluoride toothpaste. | - |
dc.type | Article | - |
dc.identifier.email | Lo, ECM: edward-lo@hku.hk | - |
dc.identifier.email | Yiu, CKY: ckyyiu@hkucc.hku.hk | - |
dc.identifier.authority | Lo, ECM=rp00015 | - |
dc.identifier.authority | Yiu, CKY=rp00018 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41598-019-44612-6 | - |
dc.identifier.pmid | 31182718 | - |
dc.identifier.scopus | eid_2-s2.0-85067210709 | - |
dc.identifier.hkuros | 303883 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 8405 | - |
dc.identifier.epage | article no. 8405 | - |
dc.identifier.isi | WOS:000470847700002 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2045-2322 | - |