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Article: Preclinical study of novel curcumin analogue SSC-5 using orthotopic tumor xenograft model for esophageal squamous cell carcinoma

TitlePreclinical study of novel curcumin analogue SSC-5 using orthotopic tumor xenograft model for esophageal squamous cell carcinoma
Authors
KeywordsCurcumin
Esophageal squamous cell carcinoma
Issue Date2018
PublisherKorean Cancer Association. The Journal's web site is located at https://www.e-crt.org/
Citation
Cancer Research and Treatment, 2018, v. 50 n. 4, p. 1362-1377 How to Cite?
AbstractPurpose Tumor xenograft model is an indispensable animal cancer model. In esophageal squamous cell carcinoma (ESCC) research, orthotopic tumor xenograft model establishes tumor xenograft in the animal esophagus, which allows the study of tumorigenesis in its native microenvironment. Materials and Methods In this study, we described two simple and reproducible methods to develop tumor xenograft at the cervical or the abdominal esophagus in nude mice by direct injection of ESCC cells in the esophageal wall. Results In comparing these two methods, the cervical one presented with more clinically relevant features, i.e., esophageal stricture, body weight loss and poor survival. In addition, the derived tumor xenografts accompanied a rapid growth rate and a high tendency to invade into the surrounding structures. This model was subsequently used to study the anti-tumor effect of curcumin, which is known for its potential therapeutic effects in various diseases including cancers, and its analogue SSC-5. SSC-5 was selected among the eight newly synthesized curcumin analogues based on its superior anti-tumor effect demonstrated in an MTT cell proliferation assay and its effects on apoptosis induction and cell cycle arrest in cultured ESCC cells. Treatment of orthotopic tumor-bearing mice with SSC-5 resulted in an inhibition in tumor growth and invasion. Conclusion Taken together, we have established a clinically relevant orthotopic tumor xenograft model that can serve as a preclinical tool for screening new anti-tumor compounds, e.g., SSC-5, in ESCC. © 2018 by the Korean Cancer Association.
Persistent Identifierhttp://hdl.handle.net/10722/274049
ISSN
2017 Impact Factor: 3.23
2015 SCImago Journal Rankings: 1.840
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTung, LN-
dc.contributor.authorSong, S-
dc.contributor.authorChan, KT-
dc.contributor.authorChoi, MY-
dc.contributor.authorLam, DHY-
dc.contributor.authorChan, CMG-
dc.contributor.authorChen, Z-
dc.contributor.authorWang, KSH-
dc.contributor.authorLeung, HT-
dc.contributor.authorLaw, SYK-
dc.contributor.authorHuang, Y-
dc.contributor.authorSong, H-
dc.contributor.authorLee, NPY-
dc.date.accessioned2019-08-18T14:54:02Z-
dc.date.available2019-08-18T14:54:02Z-
dc.date.issued2018-
dc.identifier.citationCancer Research and Treatment, 2018, v. 50 n. 4, p. 1362-1377-
dc.identifier.issn1598-2998-
dc.identifier.urihttp://hdl.handle.net/10722/274049-
dc.description.abstractPurpose Tumor xenograft model is an indispensable animal cancer model. In esophageal squamous cell carcinoma (ESCC) research, orthotopic tumor xenograft model establishes tumor xenograft in the animal esophagus, which allows the study of tumorigenesis in its native microenvironment. Materials and Methods In this study, we described two simple and reproducible methods to develop tumor xenograft at the cervical or the abdominal esophagus in nude mice by direct injection of ESCC cells in the esophageal wall. Results In comparing these two methods, the cervical one presented with more clinically relevant features, i.e., esophageal stricture, body weight loss and poor survival. In addition, the derived tumor xenografts accompanied a rapid growth rate and a high tendency to invade into the surrounding structures. This model was subsequently used to study the anti-tumor effect of curcumin, which is known for its potential therapeutic effects in various diseases including cancers, and its analogue SSC-5. SSC-5 was selected among the eight newly synthesized curcumin analogues based on its superior anti-tumor effect demonstrated in an MTT cell proliferation assay and its effects on apoptosis induction and cell cycle arrest in cultured ESCC cells. Treatment of orthotopic tumor-bearing mice with SSC-5 resulted in an inhibition in tumor growth and invasion. Conclusion Taken together, we have established a clinically relevant orthotopic tumor xenograft model that can serve as a preclinical tool for screening new anti-tumor compounds, e.g., SSC-5, in ESCC. © 2018 by the Korean Cancer Association.-
dc.languageeng-
dc.publisherKorean Cancer Association. The Journal's web site is located at https://www.e-crt.org/-
dc.relation.ispartofCancer Research and Treatment-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCurcumin-
dc.subjectEsophageal squamous cell carcinoma-
dc.titlePreclinical study of novel curcumin analogue SSC-5 using orthotopic tumor xenograft model for esophageal squamous cell carcinoma-
dc.typeArticle-
dc.identifier.emailChan, KT: ktchan66@hku.hk-
dc.identifier.emailLaw, SYK: slaw@hku.hk-
dc.identifier.emailLee, NPY: nikkilee@hku.hk-
dc.identifier.authorityLaw, SYK=rp00437-
dc.identifier.authorityLee, NPY=rp00263-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.4143/crt.2017.353-
dc.identifier.pmid29361818-
dc.identifier.scopuseid_2-s2.0-85046798851-
dc.identifier.hkuros300998-
dc.identifier.volume50-
dc.identifier.issue4-
dc.identifier.spage1362-
dc.identifier.epage1377-
dc.identifier.isiWOS:000446894300031-
dc.publisher.placeKorea, Republic of-

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