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Article: Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer

TitleOncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer
Authors
Issue Date2018
Citation
Molecular Oncology,  How to Cite?
AbstractBackground: Cervical cancer is one of the leading cause of cancer death in female worldwide, despite the current implementation of cytology / Human Papillomavirus (HPV) screening. We aimed to identify the potential role of miRNA as diagnostic biomarker in the detection of cervical pre-malignant lesions and cancer. Methods: Six miRNAs were validated in cervical cancer and pre-malignant low-grade and high-grade lesion by real-time qPCR. Mann-Whiney U-test or Kruskal-wallis test, Pearson Chi Square (2) test, Kaplan-Meier method, Logistic regression, Receiver operating characteristic (ROC) curves were used for data analyses. Results: Totally 582 patients with cervical diseases and 145 control individuals were recruited. The expressions of six miRNAs, miR-20a, miR-92a, miR-141, miR-183*, miR-210 and miR-944, were found significantly up-regulated in cervical cancer and pre-malignant lesions when compared to normal cervical samples, indicating that they are oncogenic miRNAs. ROC curve analysis showed that six miRNAs were able to discriminate patients with cervical pre-malignant lesions and cancer from normal individuals and had good predictive performance, particularly in cervical lesions. Combination of six miRNAs further enhanced the diagnostic accuracy over any single miRNA marker, with an area under the curve (AUC) of 0.998, 0.996 and 0.959, and diagnostic sensitivity of 97.9%, 97.2% and 91.4%, and specificity of 98.6%, 96.6% and 87.6% for Low-grade, high-grade lesions and cancer, respectively. Conclusions: A six oncogenic miRNA signature could be the probable diagnostic markers for cervical pre-malignant lesions and cancer and potential application in future cervical cancer screening.
Persistent Identifierhttp://hdl.handle.net/10722/260344

 

DC FieldValueLanguage
dc.contributor.authorLiu, S-
dc.contributor.authorChan, KKL-
dc.contributor.authorChu, KH-
dc.contributor.authorWei, N-
dc.contributor.authorLau, SK-
dc.contributor.authorNgu, SF-
dc.contributor.authorChu, MYM-
dc.contributor.authorTse, KY-
dc.contributor.authorIp, PCP-
dc.contributor.authorNg, KO-
dc.contributor.authorCheung, ANY-
dc.contributor.authorNgan, HYS-
dc.date.accessioned2018-09-14T08:40:07Z-
dc.date.available2018-09-14T08:40:07Z-
dc.date.issued2018-
dc.identifier.citationMolecular Oncology, -
dc.identifier.urihttp://hdl.handle.net/10722/260344-
dc.description.abstractBackground: Cervical cancer is one of the leading cause of cancer death in female worldwide, despite the current implementation of cytology / Human Papillomavirus (HPV) screening. We aimed to identify the potential role of miRNA as diagnostic biomarker in the detection of cervical pre-malignant lesions and cancer. Methods: Six miRNAs were validated in cervical cancer and pre-malignant low-grade and high-grade lesion by real-time qPCR. Mann-Whiney U-test or Kruskal-wallis test, Pearson Chi Square (2) test, Kaplan-Meier method, Logistic regression, Receiver operating characteristic (ROC) curves were used for data analyses. Results: Totally 582 patients with cervical diseases and 145 control individuals were recruited. The expressions of six miRNAs, miR-20a, miR-92a, miR-141, miR-183*, miR-210 and miR-944, were found significantly up-regulated in cervical cancer and pre-malignant lesions when compared to normal cervical samples, indicating that they are oncogenic miRNAs. ROC curve analysis showed that six miRNAs were able to discriminate patients with cervical pre-malignant lesions and cancer from normal individuals and had good predictive performance, particularly in cervical lesions. Combination of six miRNAs further enhanced the diagnostic accuracy over any single miRNA marker, with an area under the curve (AUC) of 0.998, 0.996 and 0.959, and diagnostic sensitivity of 97.9%, 97.2% and 91.4%, and specificity of 98.6%, 96.6% and 87.6% for Low-grade, high-grade lesions and cancer, respectively. Conclusions: A six oncogenic miRNA signature could be the probable diagnostic markers for cervical pre-malignant lesions and cancer and potential application in future cervical cancer screening.-
dc.languageeng-
dc.relation.ispartofMolecular Oncology-
dc.titleOncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer -
dc.typeArticle-
dc.identifier.emailLiu, S: stephasl@hku.hk-
dc.identifier.emailChan, KKL: kklchan@hkucc.hku.hk-
dc.identifier.emailChu, KH: khchu12@HKUCC-COM.hku.hk-
dc.identifier.emailWei, N: tinawei@hku.hk-
dc.identifier.emailLau, SK: lsk382@hkucc.hku.hk-
dc.identifier.emailNgu, SF: ngusiewf@hku.hk-
dc.identifier.emailChu, MYM: chumy@hku.hk-
dc.identifier.emailTse, KY: tseky@hku.hk-
dc.identifier.emailIp, PCP: philipip@hku.hk-
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hk-
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hk-
dc.identifier.authorityLiu, S=rp00372-
dc.identifier.authorityChan, KKL=rp00499-
dc.identifier.authorityNgu, SF=rp01367-
dc.identifier.authorityTse, KY=rp02391-
dc.identifier.authorityIp, PCP=rp01890-
dc.identifier.authorityCheung, ANY=rp00542-
dc.identifier.authorityNgan, HYS=rp00346-
dc.identifier.hkuros291315-

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