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Conference Paper: Significant correlation between intragraft gene expression profiles linking to acute phase injury and angoneness with HCC recurrence after LDLT
Title | Significant correlation between intragraft gene expression profiles linking to acute phase injury and angoneness with HCC recurrence after LDLT |
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Authors | |
Issue Date | 2007 |
Publisher | The International Liver Transplantation Society. |
Citation | The International Liver Transplantation Society 13th Annual International Congress, Rio de Janeiro, Brazil, 20 - 23 June 2007, p. S223 Abstract no. 559 How to Cite? |
Abstract | Objective
We aim to investigate the impact of intragraft gene expression profi les linking
to acute phase injury and angiogenesis on liver tumor recurrence of HCC
patients after LDLT.
Methods
From May 2000 to Sep 2005, 378 adult-to-adult liver transplants were
included in the current study. Liver biopsies were taken in the donors
before graft harvesting and 2 hours after reperfusion in the recipients. The
intragraft gene expression profi les of the grafts greater (Group 1) and less
than 60% (Group 2) of standard liver weight (SLW) were compared by cDNA
microarray analysis. The mRNA expression of the over-expressed genes was
further confi rmed by quantitative RT-PCR. Hepatic stellate cell activation and
intragraft gene/protein expression of Rac, FAK and CAK after reperfusion
were detected. Clinical-pathological data including the incidence of tumor
recurrence and metastases were also compared.
Results
The patients were grouped into Group 1 (>= 60% SLW, n=175) and Group 2
(<60%SLW, n=203). There were more HCC recipients in Group 2 [59 (29%)
vs 31 (17.7%), p=0.01]. There was no difference of the incidence of higher
tumor staging [21/59(35.6%) vs 13/31(41.9%), p=0.555) and patients beyond
Milan [22/59(37.3%) vs 12/31(38.7%), p=0.895] or UCSF [14/59(23.7%) vs
7/31(22.6%), p=0.903] criteria between the two groups. The incidence of liver
tumor recurrence together with lung metastasis was 22% (13/59) in Group 2.
Most of the patients with tumor recurrence had hepatic sinusoidal injury at
early phase after liver transplantation. There was no liver tumor recurrence in
Group 1 (p=0.003). Signifi cant activation of hepatic stellate cells was found
in Group 2 together with stronger intragraft protein expression of FAK and
CAK compared to that of Group 1. Intragraft mRNA levels of Egr-1, RhoA,
FAK and VEGF were also signifi cantly higher in Group 2. Distinct gene
expression profi les linking to acute phase liver graft injury (Egr-1, G-protein
signaling 5, ET-1, TGFR-b, FGFR, TNF-a and MMP-9) and angiogenesis
(angiopoietin and angiopoietin-related protein 1) were found over-expressed
in smaller liver grafts (Group 2).
Conclusion
Distinct intragraft gene expression profi les linking to acute phase injury
and angiongenesis signifi cantly correlated with higher incidence of liver
tumor recurrence and metastases in LDLT using the liver graft less than
60% of SLW. |
Persistent Identifier | http://hdl.handle.net/10722/253031 |
DC Field | Value | Language |
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dc.contributor.author | Man, K | - |
dc.contributor.author | Lo, CM | - |
dc.contributor.author | Ng, KTP | - |
dc.contributor.author | Sun, S | - |
dc.contributor.author | Sun, KW | - |
dc.contributor.author | Fan, ST | - |
dc.date.accessioned | 2018-05-10T04:50:42Z | - |
dc.date.available | 2018-05-10T04:50:42Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | The International Liver Transplantation Society 13th Annual International Congress, Rio de Janeiro, Brazil, 20 - 23 June 2007, p. S223 Abstract no. 559 | - |
dc.identifier.uri | http://hdl.handle.net/10722/253031 | - |
dc.description.abstract | Objective We aim to investigate the impact of intragraft gene expression profi les linking to acute phase injury and angiogenesis on liver tumor recurrence of HCC patients after LDLT. Methods From May 2000 to Sep 2005, 378 adult-to-adult liver transplants were included in the current study. Liver biopsies were taken in the donors before graft harvesting and 2 hours after reperfusion in the recipients. The intragraft gene expression profi les of the grafts greater (Group 1) and less than 60% (Group 2) of standard liver weight (SLW) were compared by cDNA microarray analysis. The mRNA expression of the over-expressed genes was further confi rmed by quantitative RT-PCR. Hepatic stellate cell activation and intragraft gene/protein expression of Rac, FAK and CAK after reperfusion were detected. Clinical-pathological data including the incidence of tumor recurrence and metastases were also compared. Results The patients were grouped into Group 1 (>= 60% SLW, n=175) and Group 2 (<60%SLW, n=203). There were more HCC recipients in Group 2 [59 (29%) vs 31 (17.7%), p=0.01]. There was no difference of the incidence of higher tumor staging [21/59(35.6%) vs 13/31(41.9%), p=0.555) and patients beyond Milan [22/59(37.3%) vs 12/31(38.7%), p=0.895] or UCSF [14/59(23.7%) vs 7/31(22.6%), p=0.903] criteria between the two groups. The incidence of liver tumor recurrence together with lung metastasis was 22% (13/59) in Group 2. Most of the patients with tumor recurrence had hepatic sinusoidal injury at early phase after liver transplantation. There was no liver tumor recurrence in Group 1 (p=0.003). Signifi cant activation of hepatic stellate cells was found in Group 2 together with stronger intragraft protein expression of FAK and CAK compared to that of Group 1. Intragraft mRNA levels of Egr-1, RhoA, FAK and VEGF were also signifi cantly higher in Group 2. Distinct gene expression profi les linking to acute phase liver graft injury (Egr-1, G-protein signaling 5, ET-1, TGFR-b, FGFR, TNF-a and MMP-9) and angiogenesis (angiopoietin and angiopoietin-related protein 1) were found over-expressed in smaller liver grafts (Group 2). Conclusion Distinct intragraft gene expression profi les linking to acute phase injury and angiongenesis signifi cantly correlated with higher incidence of liver tumor recurrence and metastases in LDLT using the liver graft less than 60% of SLW. | - |
dc.language | eng | - |
dc.publisher | The International Liver Transplantation Society. | - |
dc.relation.ispartof | The International Liver Transplantation Society Annual International Congress | - |
dc.title | Significant correlation between intragraft gene expression profiles linking to acute phase injury and angoneness with HCC recurrence after LDLT | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Man, K: kwanman@hku.hk | - |
dc.identifier.email | Lo, CM: chungmlo@hkucc.hku.hk | - |
dc.identifier.email | Ng, KTP: ledodes@hku.hk | - |
dc.identifier.email | Sun, S: ssun@hku.hk | - |
dc.identifier.email | Sun, KW: ckwsun@hkucc.hku.hk | - |
dc.identifier.email | Fan, ST: stfan@hku.hk | - |
dc.identifier.authority | Man, K=rp00417 | - |
dc.identifier.authority | Lo, CM=rp00412 | - |
dc.identifier.authority | Ng, KTP=rp01720 | - |
dc.identifier.authority | Fan, ST=rp00355 | - |
dc.identifier.hkuros | 135531 | - |
dc.identifier.spage | S223 Abstract no. 559 | - |
dc.identifier.epage | S223 Abstract no. 559 | - |
dc.publisher.place | Rio de Janeiro, Brazil | - |