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Article: Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

TitleModeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
Authors
Issue Date2017
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com
Citation
Journal of Visualized Experiments, 2017, n. 120, p. e55337 How to Cite?
AbstractOvarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.
Persistent Identifierhttp://hdl.handle.net/10722/245087
ISSN
2017 Impact Factor: 1.184
2015 SCImago Journal Rankings: 0.451
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, S-
dc.contributor.authorIp, KM-
dc.contributor.authorTang, YH-
dc.contributor.authorSy, KHS-
dc.contributor.authorYung, SSY-
dc.contributor.authorChan, DTM-
dc.contributor.authorYang, MS-
dc.contributor.authorShum, HC-
dc.contributor.authorWong, AST-
dc.date.accessioned2017-09-18T02:04:23Z-
dc.date.available2017-09-18T02:04:23Z-
dc.date.issued2017-
dc.identifier.citationJournal of Visualized Experiments, 2017, n. 120, p. e55337-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10722/245087-
dc.description.abstractOvarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.-
dc.languageeng-
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com-
dc.relation.ispartofJournal of Visualized Experiments-
dc.rightsJournal of Visualized Experiments. Copyright © Journal of Visualized Experiments.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleModeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice-
dc.typeArticle-
dc.identifier.emailLi, S: lss33@HKUCC-COM.hku.hk-
dc.identifier.emailTang, YH: sdmt99@connect.hku.hk-
dc.identifier.emailYung, SSY: ssyyung@hku.hk-
dc.identifier.emailChan, DTM: dtmchan@hkucc.hku.hk-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.emailWong, AST: awong1@hku.hk-
dc.identifier.authorityYung, SSY=rp00455-
dc.identifier.authorityChan, DTM=rp00394-
dc.identifier.authorityShum, HC=rp01439-
dc.identifier.authorityWong, AST=rp00805-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3791/55337-
dc.identifier.hkuros278681-
dc.identifier.issue120-
dc.identifier.spagee55337-
dc.identifier.epagee55337-
dc.identifier.isiWOS:000397847700086-
dc.publisher.placeUnited States-

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