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Conference Paper: Transcorneal electrical stimulation prevents secondary retinal ganglion cell death after acute ocular hypertensive injury through modulation of microglia-mediated local inflammatory response

TitleTranscorneal electrical stimulation prevents secondary retinal ganglion cell death after acute ocular hypertensive injury through modulation of microglia-mediated local inflammatory response
Authors
Issue Date2016
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1755-375X&site=1
Citation
The 2016 Annual Meeting of the European Association for Vision and Eye Research (EVER 2016), Nice, France, 5-8 October 2016. In Acta Ophthalmologica, 2016, v. 94 n. S256 How to Cite?
AbstractPURPOSE: To investigate neuroprotective effect of transcorneal electrical stimulation (TcES) on retinal ganglion cells (RGC) after acute ocular hypertension related retinal injury in gerbils. METHODS: The right eye of each gerbil was used for experiments. Acue ocular hypertensive injury was induced via intracameral infusion of basic salt solution. Intraocular pressure (IOP) was adjusted by changing the bottle height position. For all gerbis, right eye IOP was elevated to 85 mmHg for 1 h. In the treatment group, TcES was applied to the surgical eye immediately and then twice weekly for a total of 1 month. In the control group, sham TcES was given to the surgical eye during the same time points. Retinal function was assessed and compared between groups using a full field flash electroretinogram and a light/dark transition box test. For histological analysis, the number of RGCs, astrocytes and microglial cells were counted by immunofluorescence staining after the gerbils were sacrificed on day 7 and day 28. Real time PCR and western blot analysis were conducted to compare expression of IL-10, IL-6, Cox-2, TNF-α and NFκB phorsphorylation between groups. RESULTS: TcES treated eyes had significantly higher RGC survival at 1 month compared to controls. This was associated with improved RGC function and vision-related behaviour. Furthermore TcES treated eyes were shown to have increased IL-10 expression, with a corresponding reduction in IL-6 and COX-2 expression as well as reduction in NF-kB phosphorylation. This was associated with a suppression in microglial cell activation in TcES treated eyes. CONCLUSIONS: Early treatment with TcES in gerbils protected the RGCs from damage and preserved retinal function in acute ocular hypertensive injury through modulation of the microglial-cell activated local inflammatory response.
DescriptionThis free journal suppl. entitled: Special Issue: Abstracts from the 2016 European Association for Vision and Eye Research Conference
Persistent Identifierhttp://hdl.handle.net/10722/234373
ISSN
2015 Impact Factor: 3.032
2015 SCImago Journal Rankings: 1.473

 

DC FieldValueLanguage
dc.contributor.authorShih, KC-
dc.contributor.authorFu, L-
dc.contributor.authorLo, ACY-
dc.contributor.authorLai, JSM-
dc.date.accessioned2016-10-14T13:46:25Z-
dc.date.available2016-10-14T13:46:25Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Annual Meeting of the European Association for Vision and Eye Research (EVER 2016), Nice, France, 5-8 October 2016. In Acta Ophthalmologica, 2016, v. 94 n. S256-
dc.identifier.issn1755-375X-
dc.identifier.urihttp://hdl.handle.net/10722/234373-
dc.descriptionThis free journal suppl. entitled: Special Issue: Abstracts from the 2016 European Association for Vision and Eye Research Conference-
dc.description.abstractPURPOSE: To investigate neuroprotective effect of transcorneal electrical stimulation (TcES) on retinal ganglion cells (RGC) after acute ocular hypertension related retinal injury in gerbils. METHODS: The right eye of each gerbil was used for experiments. Acue ocular hypertensive injury was induced via intracameral infusion of basic salt solution. Intraocular pressure (IOP) was adjusted by changing the bottle height position. For all gerbis, right eye IOP was elevated to 85 mmHg for 1 h. In the treatment group, TcES was applied to the surgical eye immediately and then twice weekly for a total of 1 month. In the control group, sham TcES was given to the surgical eye during the same time points. Retinal function was assessed and compared between groups using a full field flash electroretinogram and a light/dark transition box test. For histological analysis, the number of RGCs, astrocytes and microglial cells were counted by immunofluorescence staining after the gerbils were sacrificed on day 7 and day 28. Real time PCR and western blot analysis were conducted to compare expression of IL-10, IL-6, Cox-2, TNF-α and NFκB phorsphorylation between groups. RESULTS: TcES treated eyes had significantly higher RGC survival at 1 month compared to controls. This was associated with improved RGC function and vision-related behaviour. Furthermore TcES treated eyes were shown to have increased IL-10 expression, with a corresponding reduction in IL-6 and COX-2 expression as well as reduction in NF-kB phosphorylation. This was associated with a suppression in microglial cell activation in TcES treated eyes. CONCLUSIONS: Early treatment with TcES in gerbils protected the RGCs from damage and preserved retinal function in acute ocular hypertensive injury through modulation of the microglial-cell activated local inflammatory response.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1755-375X&site=1-
dc.relation.ispartofActa Ophthalmologica-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article]. Authors are not required to remove preprints posted prior to acceptance of the submitted version. Postprint This is the accepted version of the following article: [full citation], which has been published in final form at [Link to final article].-
dc.titleTranscorneal electrical stimulation prevents secondary retinal ganglion cell death after acute ocular hypertensive injury through modulation of microglia-mediated local inflammatory response-
dc.typeConference_Paper-
dc.identifier.emailShih, KC: kcshih@hku.hk-
dc.identifier.emailLo, ACY: amylo@hku.hk-
dc.identifier.emailLai, JSM: laism@hku.hk-
dc.identifier.authorityShih, KC=rp01374-
dc.identifier.authorityLo, ACY=rp00425-
dc.identifier.authorityLai, JSM=rp00295-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1755-3768.2016.0315-
dc.identifier.hkuros268980-
dc.identifier.volume94-
dc.identifier.issueS256-
dc.publisher.placeUnited States-

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