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Conference Paper: Orexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats

TitleOrexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats
Authors
Issue Date2016
PublisherThe University of Hong Kong.
Citation
The 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 37, abstract no. P16 How to Cite?
AbstractOrexin is a neuromodulator known to be produced by lateral hypothalamic neurons, which send projections to the hippocampus, brainstem and cerebellum. In the hippocampus, orexin is known to modulate long-term synaptic plasticity, thereby contributing to social memory. We hypothesized that orexin modulates synaptic transmission in the vestibular nucleus (VN), thus regulating behavioral expression. To understand the impact of orexin on synaptic transmission within the VN, we employed an established in vitro electrophysiological technique to study the action of orexin on the excitability of neurons in the medial vestibular nucleus (MVN) of rats at postnatal day 14. Whole-cell patch-clamp results indicated that treatment with orexin led to reductions in amplitude and frequency of miniature inhibitory postsynaptic current (mIPSC). This suggests that orexin decreases both the presynaptic release of inhibitory transmitters and postsynaptic depolarization in MVN neurons. We thus demonstrated that orexin attenuates synaptic inhibition on MVN neurons. We further tested whether orexin-modulated mIPSC is mediated by GABAA receptors or glycine receptors. With the use of bicuculline and strychnine, we observed that orexin decreased mIPSC mediated by GABAA receptors, but not glycine receptors. Taken together, our findings provided us with fundamental knowledge about the modulatory role of orexinergic transmission in the VN. This forms the basis for further investigation on the involvement of orexin in long-term synaptic plasticity of the central vestibular system. [Supported by N_HKU735/14]
DescriptionConference Theme: Nature and Nurture in Brain Functions
Persistent Identifierhttp://hdl.handle.net/10722/231498

 

DC FieldValueLanguage
dc.contributor.authorJiang, Y-
dc.contributor.authorMa, CW-
dc.contributor.authorWang, JJ-
dc.contributor.authorChan, YS-
dc.date.accessioned2016-09-20T05:23:33Z-
dc.date.available2016-09-20T05:23:33Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Neuroscience Symposium and Annual Scientific Conference of the Hong Kong Society of Neurosciences, The University of Hong Kong, Hong Kong, 18 May 2016. In Programme Book, 2016, p. 37, abstract no. P16-
dc.identifier.urihttp://hdl.handle.net/10722/231498-
dc.descriptionConference Theme: Nature and Nurture in Brain Functions-
dc.description.abstractOrexin is a neuromodulator known to be produced by lateral hypothalamic neurons, which send projections to the hippocampus, brainstem and cerebellum. In the hippocampus, orexin is known to modulate long-term synaptic plasticity, thereby contributing to social memory. We hypothesized that orexin modulates synaptic transmission in the vestibular nucleus (VN), thus regulating behavioral expression. To understand the impact of orexin on synaptic transmission within the VN, we employed an established in vitro electrophysiological technique to study the action of orexin on the excitability of neurons in the medial vestibular nucleus (MVN) of rats at postnatal day 14. Whole-cell patch-clamp results indicated that treatment with orexin led to reductions in amplitude and frequency of miniature inhibitory postsynaptic current (mIPSC). This suggests that orexin decreases both the presynaptic release of inhibitory transmitters and postsynaptic depolarization in MVN neurons. We thus demonstrated that orexin attenuates synaptic inhibition on MVN neurons. We further tested whether orexin-modulated mIPSC is mediated by GABAA receptors or glycine receptors. With the use of bicuculline and strychnine, we observed that orexin decreased mIPSC mediated by GABAA receptors, but not glycine receptors. Taken together, our findings provided us with fundamental knowledge about the modulatory role of orexinergic transmission in the VN. This forms the basis for further investigation on the involvement of orexin in long-term synaptic plasticity of the central vestibular system. [Supported by N_HKU735/14]-
dc.languageeng-
dc.publisherThe University of Hong Kong.-
dc.relation.ispartofNeuroscience Symposium & Annual Scientific Conference of the Hong Kong Society of Neurosciences-
dc.titleOrexin modulates inhibitory synaptic transmission of vestibular nuclear neurons in rats-
dc.typeConference_Paper-
dc.identifier.emailMa, CW: cwma2010@hku.hk-
dc.identifier.emailWang, JJ: junwen@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityWang, JJ=rp00280-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.hkuros266238-
dc.identifier.hkuros267893-
dc.identifier.spage37, abstract no. P16-
dc.identifier.epage37, abstract no. P16-
dc.publisher.placeHong Kong-

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