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Conference Paper: Quantitative asymmetric-detection time-stretch optical microscopy (Q-ATOM) for ultrafast quantitative phase imaging flow cytometry

TitleQuantitative asymmetric-detection time-stretch optical microscopy (Q-ATOM) for ultrafast quantitative phase imaging flow cytometry
Authors
Issue Date2016
PublisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2
Citation
The 2016 SPIE Photonics West Conference, San Francisco, CA., 13-18 February 2016. In Conference Proceedings, 2016, v. 9720, paper no. 9720-33 How to Cite?
AbstractBased on the interferometric or holographic approaches, recent QPM techniques provide quantitative-phase information, e.g cell volume, dry mass and optical scattering properties for label-free cellular physical phenotyping. These approaches generally rely on iterative phase-retrieval algorithms to obtain quantitative-phase information, which are computationally intensive. Moreover, current QPM techniques can only offer limited image acquisition rate by using CMOS/CCD image sensors, these two limitations hinder QPM for high-throughput quantitative image-based single-cell analysis in real-time. To this end, we demonstrate an interferometry-free quantitative phase microscopy developed on a new generation of time-stretch microscopy, asymmetric-detection time-stretch optical microscopy (ATOM), which is coined quantitative ATOM (Q-ATOM) - featuring an unprecedented cell measurement throughput together with the assorted intrinsic optical phenotypes (e.g. angular light scattering profile) and the derived physical properties of the cells (e.g. cell size, dry mass density etc.). Based on a similar concept to Schlieren imaging, Q-ATOM retrieves quantitative-phase information through multiple off-axis light-beam detection at a line-scan rate of >10 MHz - a speed unachievable by any existing QPM techniques. Phase retrieval in Q-ATOM relies on a non-iterative method, significantly reducing the computational complexity of the technique. It is a particularly important feature which facilitates real-time continuous label-free single-cell analysis in Q-ATOM. With the use of a non-interferometric configuration, we demonstrate ultrafast Q-ATOM of mouse chondrocytes and hypertrophic chondrocytes in ultrafast microfluidic flow with sub-cellular resolution at an imaging throughput equivalent to ~100,000 cells/sec without image blur. This technique shows a great potential for ultrahigh throughput label-free image-based single-cell biophysical phentotyping.
DescriptionBiomedical Spectroscopy, Microscopy, and Imaging: 9720 - High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management: Paper 9720-33
Persistent Identifierhttp://hdl.handle.net/10722/229830
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLau, AKS-
dc.contributor.authorTang, AHL-
dc.contributor.authorChung, BMF-
dc.contributor.authorTsang, KY-
dc.contributor.authorChan, ACS-
dc.contributor.authorWei, X-
dc.contributor.authorWong, KKY-
dc.contributor.authorLam, EYM-
dc.contributor.authorCheah, KSE-
dc.contributor.authorShum, AHC-
dc.contributor.authorTsia, KKM-
dc.date.accessioned2016-08-23T14:13:30Z-
dc.date.available2016-08-23T14:13:30Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 SPIE Photonics West Conference, San Francisco, CA., 13-18 February 2016. In Conference Proceedings, 2016, v. 9720, paper no. 9720-33-
dc.identifier.issn0277-786X-
dc.identifier.urihttp://hdl.handle.net/10722/229830-
dc.descriptionBiomedical Spectroscopy, Microscopy, and Imaging: 9720 - High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management: Paper 9720-33-
dc.description.abstractBased on the interferometric or holographic approaches, recent QPM techniques provide quantitative-phase information, e.g cell volume, dry mass and optical scattering properties for label-free cellular physical phenotyping. These approaches generally rely on iterative phase-retrieval algorithms to obtain quantitative-phase information, which are computationally intensive. Moreover, current QPM techniques can only offer limited image acquisition rate by using CMOS/CCD image sensors, these two limitations hinder QPM for high-throughput quantitative image-based single-cell analysis in real-time. To this end, we demonstrate an interferometry-free quantitative phase microscopy developed on a new generation of time-stretch microscopy, asymmetric-detection time-stretch optical microscopy (ATOM), which is coined quantitative ATOM (Q-ATOM) - featuring an unprecedented cell measurement throughput together with the assorted intrinsic optical phenotypes (e.g. angular light scattering profile) and the derived physical properties of the cells (e.g. cell size, dry mass density etc.). Based on a similar concept to Schlieren imaging, Q-ATOM retrieves quantitative-phase information through multiple off-axis light-beam detection at a line-scan rate of >10 MHz - a speed unachievable by any existing QPM techniques. Phase retrieval in Q-ATOM relies on a non-iterative method, significantly reducing the computational complexity of the technique. It is a particularly important feature which facilitates real-time continuous label-free single-cell analysis in Q-ATOM. With the use of a non-interferometric configuration, we demonstrate ultrafast Q-ATOM of mouse chondrocytes and hypertrophic chondrocytes in ultrafast microfluidic flow with sub-cellular resolution at an imaging throughput equivalent to ~100,000 cells/sec without image blur. This technique shows a great potential for ultrahigh throughput label-free image-based single-cell biophysical phentotyping.-
dc.languageeng-
dc.publisherSPIE - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2-
dc.relation.ispartofSPIE - International Society for Optical Engineering Proceedings-
dc.rightsSPIE - International Society for Optical Engineering Proceedings. Copyright © SPIE - International Society for Optical Engineering.-
dc.titleQuantitative asymmetric-detection time-stretch optical microscopy (Q-ATOM) for ultrafast quantitative phase imaging flow cytometry-
dc.typeConference_Paper-
dc.identifier.emailLau, AKS: andylks@hku.hk-
dc.identifier.emailTsang, KY: kytsang@hku.hk-
dc.identifier.emailWei, X: xmwei@hku.hk-
dc.identifier.emailWong, KKY: kywong@eee.hku.hk-
dc.identifier.emailLam, EYM: elam@eee.hku.hk-
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hk-
dc.identifier.emailShum, AHC: ashum@hku.hk-
dc.identifier.emailTsia, KKM: tsia@hku.hk-
dc.identifier.authorityWong, KKY=rp00189-
dc.identifier.authorityLam, EYM=rp00131-
dc.identifier.authorityCheah, KSE=rp00342-
dc.identifier.authorityShum, AHC=rp01439-
dc.identifier.authorityTsia, KKM=rp01389-
dc.identifier.doi10.1117/12.2212116-
dc.identifier.hkuros262138-
dc.identifier.hkuros267554-
dc.identifier.volume9720, paper no. 9720-33-
dc.publisher.placeUnited States-
dc.description.otherThe 2016 SPIE Photonics West Conference, San Francisco, CA., 13-18 February 2016. In Conference Proceedings, 2016, v. 9720, paper no. 9720-33-

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