File Download

There are no files associated with this item.

Conference Paper: Bitter melon (Momordica charantia) extract inhibits tumorigenicity and overcomes cisplatin-resistance in ovarian cancer cells through targeting AMPK signaling cascade

TitleBitter melon (Momordica charantia) extract inhibits tumorigenicity and overcomes cisplatin-resistance in ovarian cancer cells through targeting AMPK signaling cascade
Authors
Issue Date2015
Citation
HKU Obstetrics and Gynaecology 2015, Symposium on Past, Present and Future of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 14-15 November 2015. How to Cite?
AbstractObjective: Acquired chemoresistance is a major obstacle in clinical management of ovarian cancer. Therefore, searching for alternative therapeutic modalities is urgently needed. Bitter melon (Momordica charantia) is a traditional dietary fruit, but its extract also shows potential medicinal uses in human diabetes and cancers. Here, we sought to investigate the extract of bitter melon (BME) in anti-tumorigenic and cisplatin-induced cytotoxicity in ovarian cancer cells. Methods: Three varieties of bitter melon were used to prepare the bitter melon extract (BME). Ovarian cancer cell lines, human immortalized epithelial ovarian cells (HOSEs) and nude mice were used to evaluate the cell cytotoxicity, cisplatin-resistance and the tumor inhibitory effect of BME. The molecular mechanism of BME was examined by western blotting. Results: Co-treatment with BME and cisplatin markedly attenuated tumor growth in vitro and in vivo in a mouse xenograft model, while there was no observable toxicity in human immortalized epithelial ovarian cells (HOSEs) or in nude mice in vivo. Interestingly, the anti-tumorigenic effects of BME varied with different varieties of bitter melon, suggesting that the amount of anti-tumorigenic substances may vary. Studies of the molecular mechanism demonstrated that BME activates AMPK in an AMP-independent, but CaMKK-dependent manner, exerting anti-cancer effects through activation of AMPK and suppression of the mTOR/p70S6K and/or the AKT/ERK/FOXM1 signaling cascade. Conclusion: BME functions as a natural AMPK activator in inhibition of ovarian cancer cell growth, and might be useful as a supplement to improve the efficacy of cisplatin-based chemotherapy in ovarian cancer.
Persistent Identifierhttp://hdl.handle.net/10722/227675

 

DC FieldValueLanguage
dc.contributor.authorYung, MH-
dc.contributor.authorLeung, THY-
dc.contributor.authorNgan, HYS-
dc.contributor.authorChan, DW-
dc.date.accessioned2016-07-18T09:12:10Z-
dc.date.available2016-07-18T09:12:10Z-
dc.date.issued2015-
dc.identifier.citationHKU Obstetrics and Gynaecology 2015, Symposium on Past, Present and Future of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 14-15 November 2015.-
dc.identifier.urihttp://hdl.handle.net/10722/227675-
dc.description.abstractObjective: Acquired chemoresistance is a major obstacle in clinical management of ovarian cancer. Therefore, searching for alternative therapeutic modalities is urgently needed. Bitter melon (Momordica charantia) is a traditional dietary fruit, but its extract also shows potential medicinal uses in human diabetes and cancers. Here, we sought to investigate the extract of bitter melon (BME) in anti-tumorigenic and cisplatin-induced cytotoxicity in ovarian cancer cells. Methods: Three varieties of bitter melon were used to prepare the bitter melon extract (BME). Ovarian cancer cell lines, human immortalized epithelial ovarian cells (HOSEs) and nude mice were used to evaluate the cell cytotoxicity, cisplatin-resistance and the tumor inhibitory effect of BME. The molecular mechanism of BME was examined by western blotting. Results: Co-treatment with BME and cisplatin markedly attenuated tumor growth in vitro and in vivo in a mouse xenograft model, while there was no observable toxicity in human immortalized epithelial ovarian cells (HOSEs) or in nude mice in vivo. Interestingly, the anti-tumorigenic effects of BME varied with different varieties of bitter melon, suggesting that the amount of anti-tumorigenic substances may vary. Studies of the molecular mechanism demonstrated that BME activates AMPK in an AMP-independent, but CaMKK-dependent manner, exerting anti-cancer effects through activation of AMPK and suppression of the mTOR/p70S6K and/or the AKT/ERK/FOXM1 signaling cascade. Conclusion: BME functions as a natural AMPK activator in inhibition of ovarian cancer cell growth, and might be useful as a supplement to improve the efficacy of cisplatin-based chemotherapy in ovarian cancer.-
dc.languageeng-
dc.relation.ispartofHKU O & G 90th Anniversary Celebration-Symposium: Past, Present and Future of Obstetrics and Gynaecology-
dc.titleBitter melon (Momordica charantia) extract inhibits tumorigenicity and overcomes cisplatin-resistance in ovarian cancer cells through targeting AMPK signaling cascade-
dc.typeConference_Paper-
dc.identifier.emailYung, MH: mhyung@hku.hk-
dc.identifier.emailLeung, THY: thyl@hkucc.hku.hk-
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hk-
dc.identifier.emailChan, DW: dwchan@hku.hk-
dc.identifier.authorityNgan, HYS=rp00346-
dc.identifier.authorityChan, DW=rp00543-
dc.identifier.hkuros259646-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats