File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Nicotinic acetylcholine receptor expression in human airway correlates with lung function

TitleNicotinic acetylcholine receptor expression in human airway correlates with lung function
Authors
Issue Date2016
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajplung.physiology.org/
Citation
American Journal of Physiology: Lung Cellular and Molecular Physiology, 2016, v. 310 n. 3, p. L232-L239 How to Cite?
AbstractNicotine and its derivatives, by binding to nicotinic acetylcholine receptors (nAChRs) on bronchial epithelial cells, can regulate cellular signaling and inflammatory processes. Delineation of nAChR subtypes and their responses to nicotine stimulation in bronchial epithelium may provide information for therapeutic targeting in smoking-related inflammation in the airway. Expression of nAChR subunit genes in 60 bronchial epithelial biopsies and immunohistochemical staining for the subcellular locations of nAChR subunit expression were evaluated. Seven human bronchial epithelial cell lines (HBECs) were exposed to nicotine in vitro for their response in nAChR subunit gene expression to nicotine exposure and removal. The relative normalized amount of expression of nAChR α4, α5, and α7 and immunohistochemical staining intensity of nAChR α4, α5, and β3 expression showed significant correlation with lung function parameters. Nicotine stimulation in HBECs resulted in transient increase in the levels of nAChR α5 and α6 but more sustained increase in nAChR α7 expression. nAChR expression in bronchial epithelium was found to correlate with lung function. Nicotine exposure in HBECs resulted in both short and longer term responses in nAChR subunit gene expression. These results gave insight into the potential of targeting nAChRs for therapy in smoking-related inflammation in the airway.
Persistent Identifierhttp://hdl.handle.net/10722/224926
ISSN
2015 Impact Factor: 4.721
2015 SCImago Journal Rankings: 1.838

 

DC FieldValueLanguage
dc.contributor.authorLam, DCL-
dc.contributor.authorLuo, SY-
dc.contributor.authorFu, KH-
dc.contributor.authorLui, MMS-
dc.contributor.authorChan, KH-
dc.contributor.authorWistuba, II-
dc.contributor.authorGao, B-
dc.contributor.authorTsao, GSW-
dc.contributor.authorIp, MSM-
dc.contributor.authorMinna, JD-
dc.date.accessioned2016-04-18T03:34:08Z-
dc.date.available2016-04-18T03:34:08Z-
dc.date.issued2016-
dc.identifier.citationAmerican Journal of Physiology: Lung Cellular and Molecular Physiology, 2016, v. 310 n. 3, p. L232-L239-
dc.identifier.issn1040-0605-
dc.identifier.urihttp://hdl.handle.net/10722/224926-
dc.description.abstractNicotine and its derivatives, by binding to nicotinic acetylcholine receptors (nAChRs) on bronchial epithelial cells, can regulate cellular signaling and inflammatory processes. Delineation of nAChR subtypes and their responses to nicotine stimulation in bronchial epithelium may provide information for therapeutic targeting in smoking-related inflammation in the airway. Expression of nAChR subunit genes in 60 bronchial epithelial biopsies and immunohistochemical staining for the subcellular locations of nAChR subunit expression were evaluated. Seven human bronchial epithelial cell lines (HBECs) were exposed to nicotine in vitro for their response in nAChR subunit gene expression to nicotine exposure and removal. The relative normalized amount of expression of nAChR α4, α5, and α7 and immunohistochemical staining intensity of nAChR α4, α5, and β3 expression showed significant correlation with lung function parameters. Nicotine stimulation in HBECs resulted in transient increase in the levels of nAChR α5 and α6 but more sustained increase in nAChR α7 expression. nAChR expression in bronchial epithelium was found to correlate with lung function. Nicotine exposure in HBECs resulted in both short and longer term responses in nAChR subunit gene expression. These results gave insight into the potential of targeting nAChRs for therapy in smoking-related inflammation in the airway.-
dc.languageeng-
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajplung.physiology.org/-
dc.relation.ispartofAmerican Journal of Physiology: Lung Cellular and Molecular Physiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleNicotinic acetylcholine receptor expression in human airway correlates with lung function-
dc.typeArticle-
dc.identifier.emailLam, DCL: dcllam@hku.hk-
dc.identifier.emailLuo, SY: susanyl@hku.hk-
dc.identifier.emailChan, KH: koonho@hku.hk-
dc.identifier.emailTsao, GSW: gswtsao@hku.hk-
dc.identifier.emailIp, MSM: msmip@hku.hk-
dc.identifier.authorityLam, DCL=rp01345-
dc.identifier.authorityChan, KH=rp00537-
dc.identifier.authorityTsao, GSW=rp00399-
dc.identifier.authorityIp, MSM=rp00347-
dc.description.naturepostprint-
dc.identifier.doi10.1152/ajplung.00101.2015-
dc.identifier.scopuseid_2-s2.0-84956895664-
dc.identifier.hkuros257408-
dc.identifier.volume310-
dc.identifier.issue3-
dc.identifier.spageL232-
dc.identifier.epageL239-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats