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Article: MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility

TitleMicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility
Authors
Issue Date2015
Citation
Frontiers of Medicine, 2015, v. 9 n. 3, p. 331-343 How to Cite?
AbstractMicroRNAs (miRNAs), an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level. miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma (HCC) and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement. miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3'- and 5'-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.
Persistent Identifierhttp://hdl.handle.net/10722/219204
ISSN
2015 Impact Factor: 1.863
2015 SCImago Journal Rankings: 0.995

 

DC FieldValueLanguage
dc.contributor.authorTsang, FH-
dc.contributor.authorAu, LKS-
dc.contributor.authorWei, L-
dc.contributor.authorFan, NY-
dc.contributor.authorLee, MF-
dc.contributor.authorWong, CCL-
dc.contributor.authorNg, IOL-
dc.contributor.authorWong, CM-
dc.date.accessioned2015-09-18T07:17:27Z-
dc.date.available2015-09-18T07:17:27Z-
dc.date.issued2015-
dc.identifier.citationFrontiers of Medicine, 2015, v. 9 n. 3, p. 331-343-
dc.identifier.issn2095-0217-
dc.identifier.urihttp://hdl.handle.net/10722/219204-
dc.description.abstractMicroRNAs (miRNAs), an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level. miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma (HCC) and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement. miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3'- and 5'-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.-
dc.languageeng-
dc.relation.ispartofFrontiers of Medicine-
dc.titleMicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility-
dc.typeArticle-
dc.identifier.emailLee, MF: joyce@pathology.hku.hk-
dc.identifier.emailWong, CCL: carmencl@pathology.hku.hk-
dc.identifier.emailNg, IOL: iolng@hku.hk-
dc.identifier.emailWong, CM: jcmwong@hkucc.hku.hk-
dc.identifier.authorityWong, CCL=rp01602-
dc.identifier.authorityNg, IOL=rp00335-
dc.identifier.authorityWong, CM=rp00231-
dc.identifier.doi10.1007/s11684-015-0409-8-
dc.identifier.hkuros252157-
dc.identifier.hkuros262258-
dc.identifier.volume9-
dc.identifier.issue3-
dc.identifier.spage331-
dc.identifier.epage343-

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