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Article: Deficiency of adipocyte fatty-acid-binding protein alleviates myocardial ischaemia/reperfusion injury and diabetes-induced cardiac dysfunction

TitleDeficiency of adipocyte fatty-acid-binding protein alleviates myocardial ischaemia/reperfusion injury and diabetes-induced cardiac dysfunction
Authors
Issue Date2015
PublisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/
Citation
Clinical Science, 2015, v. 129 n. 7, p. 547-559 How to Cite?
AbstractClinical evidence shows that circulating levels of adipocyte fatty-acid-binding protein (A-FABP) are elevated in patients with diabetes and closely associated with ischaemic heart disease. Patients with diabetes are more susceptible to myocardial ischaemia/reperfusion (MI/R) injury. The experiments in the present study investigated the role of A-FABP in MI/R injury with or without diabetes. Non-diabetic and diabetic (streptozotocin-induced) A-FABP knockout and wild-type mice were subjected to MI/R or sham intervention. After MI/R, A-FABP knockout mice exhibited reductions in myocardial infarct size, apoptotic index, oxidative and nitrative stress, and inflammation. These reductions were accompanied by an improved left ventricular function compared with the relative controls under non-diabetic or diabetic conditions. After diabetes induction, A-FABP knockout mice exhibited a preserved cardiac function compared with that in wild-type mice. Endothelial cells, but not cardiomyocytes, were identified as the most likely source of cardiac A-FABP. Cardiac and circulating A-FABP levels were significantly increased in mice with diabetes or MI/R. Diabetes-induced superoxide anion production was significantly elevated in wild-type mice, but diminished in A-FABP knockout mice, and this elevation contributed to the exaggeration of MI/R-induced cardiac injury. Phosphorylation of endothelial nitric oxide synthase (eNOS) and production of nitric oxide (NO) were enhanced in both diabetic and non-diabetic A-FABP knockout mice after MI/R injury, but diminished in wild-type mice. The beneficial effects of A-FABP deficiency on MI/R injury were abolished by the NOS inhibitor N(G)-nitro-L-arginine methyl ester. Thus, A-FABP deficiency protects mice against MI/R-induced and/or diabetes-induced cardiac injury at least partially through activation of the eNOS/NO pathway and reduction in superoxide anion production.
Persistent Identifierhttp://hdl.handle.net/10722/215068
ISSN
2015 Impact Factor: 4.996
2015 SCImago Journal Rankings: 2.427

 

DC FieldValueLanguage
dc.contributor.authorZhou, M-
dc.contributor.authorBao, Y-
dc.contributor.authorLi, H-
dc.contributor.authorPan, Y-
dc.contributor.authorShu, L-
dc.contributor.authorXia, Z-
dc.contributor.authorWu, D-
dc.contributor.authorLam, KSL-
dc.contributor.authorVanhoutte, PMGR-
dc.contributor.authorXu, A-
dc.contributor.authorJia, W-
dc.contributor.authorHoo, RLC-
dc.date.accessioned2015-08-21T12:23:47Z-
dc.date.available2015-08-21T12:23:47Z-
dc.date.issued2015-
dc.identifier.citationClinical Science, 2015, v. 129 n. 7, p. 547-559-
dc.identifier.issn0143-5221-
dc.identifier.urihttp://hdl.handle.net/10722/215068-
dc.description.abstractClinical evidence shows that circulating levels of adipocyte fatty-acid-binding protein (A-FABP) are elevated in patients with diabetes and closely associated with ischaemic heart disease. Patients with diabetes are more susceptible to myocardial ischaemia/reperfusion (MI/R) injury. The experiments in the present study investigated the role of A-FABP in MI/R injury with or without diabetes. Non-diabetic and diabetic (streptozotocin-induced) A-FABP knockout and wild-type mice were subjected to MI/R or sham intervention. After MI/R, A-FABP knockout mice exhibited reductions in myocardial infarct size, apoptotic index, oxidative and nitrative stress, and inflammation. These reductions were accompanied by an improved left ventricular function compared with the relative controls under non-diabetic or diabetic conditions. After diabetes induction, A-FABP knockout mice exhibited a preserved cardiac function compared with that in wild-type mice. Endothelial cells, but not cardiomyocytes, were identified as the most likely source of cardiac A-FABP. Cardiac and circulating A-FABP levels were significantly increased in mice with diabetes or MI/R. Diabetes-induced superoxide anion production was significantly elevated in wild-type mice, but diminished in A-FABP knockout mice, and this elevation contributed to the exaggeration of MI/R-induced cardiac injury. Phosphorylation of endothelial nitric oxide synthase (eNOS) and production of nitric oxide (NO) were enhanced in both diabetic and non-diabetic A-FABP knockout mice after MI/R injury, but diminished in wild-type mice. The beneficial effects of A-FABP deficiency on MI/R injury were abolished by the NOS inhibitor N(G)-nitro-L-arginine methyl ester. Thus, A-FABP deficiency protects mice against MI/R-induced and/or diabetes-induced cardiac injury at least partially through activation of the eNOS/NO pathway and reduction in superoxide anion production.-
dc.languageeng-
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/-
dc.relation.ispartofClinical Science-
dc.rightsThe final version of record is available at http://www.clinsci.org/content/129/7/547-
dc.titleDeficiency of adipocyte fatty-acid-binding protein alleviates myocardial ischaemia/reperfusion injury and diabetes-induced cardiac dysfunction-
dc.typeArticle-
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hk-
dc.identifier.emailLam, KSL: ksllam@hku.hk-
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hk-
dc.identifier.emailXu, A: amxu@hkucc.hku.hk-
dc.identifier.emailHoo, RLC: rubyhoo@hku.hk-
dc.identifier.authorityXia, Z=rp00532-
dc.identifier.authorityLam, KSL=rp00343-
dc.identifier.authorityVanhoutte, PMGR=rp00238-
dc.identifier.authorityXu, A=rp00485-
dc.identifier.authorityHoo, RLC=rp01334-
dc.identifier.doi10.1042/CS20150073-
dc.identifier.pmid26186740-
dc.identifier.hkuros246875-
dc.identifier.hkuros253320-
dc.identifier.hkuros261659-
dc.identifier.hkuros261680-
dc.identifier.volume129-
dc.identifier.issue7-
dc.identifier.spage547-
dc.identifier.epage559-
dc.publisher.placeUnited Kingdom-

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