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- Publisher Website: 10.18632/oncotarget.3700
- Scopus: eid_2-s2.0-84931075204
- PMID: 25909223
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Article: MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis
Title | MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis |
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Authors | |
Keywords | Cancer metastasis Cytoskeletal reorganization Hepatocellular carcinoma mir-200 family RHO/ROCK signaling pathway |
Issue Date | 2015 |
Publisher | Impact Journals LLC. The Journal's web site is located at http://www.impactjournals.com/oncotarget/index.html |
Citation | Oncotarget, 2015, v. 6 n. 15, p. 13658-13670 How to Cite? |
Abstract | MiR-200 family is an important regulator of epithelial-mesenchymal transition and has been implicated in human carcinogenesis. However, their expression and functions in human cancers remain controversial. In the work presented here, we showed that miR-200 family members were frequently down-regulated in hepatocellular carcinoma (HCC). Although all five members of miR-200 family inhibited ZEB1/2 expression in HCC cell lines, we showed that overexpression only of the miR-200b/200c/429 subfamily, but not the miR-200a/141 subfamily, resulted in impeded HCC cell migration. Further investigations led to the identification of RhoA and ROCK2 as specific down-stream targets of the miR-200b/200c/429 subfamily. We demonstrated that the miR-200b/200c/429 subfamily inhibited HCC cell migration through modulating Rho/ROCK mediated cell cytoskeletal reorganization and cell-substratum adhesion. Re-expression of miR-200b significantly suppressed lung metastasis of HCC cells in an orthotopic liver implantation model in vivo. In conclusion, our findings identified the miR-200b/200c/429 subfamily as metastasis suppressor microRNAs in human HCC and highlighted the functional discrepancy among miR-200 family members. |
Persistent Identifier | http://hdl.handle.net/10722/214731 |
ISSN | 2016 Impact Factor: 5.168 2023 SCImago Journal Rankings: 0.789 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wong, CM | - |
dc.contributor.author | Wei, L | - |
dc.contributor.author | Au, SLK | - |
dc.contributor.author | Fan, DNY | - |
dc.contributor.author | Zhou, Y | - |
dc.contributor.author | Tsang, FHC | - |
dc.contributor.author | Law, CT | - |
dc.contributor.author | Lee, JMF | - |
dc.contributor.author | He, X | - |
dc.contributor.author | Shi, J | - |
dc.contributor.author | Wong, CCL | - |
dc.contributor.author | Ng, IOL | - |
dc.date.accessioned | 2015-08-21T11:53:23Z | - |
dc.date.available | 2015-08-21T11:53:23Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Oncotarget, 2015, v. 6 n. 15, p. 13658-13670 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | http://hdl.handle.net/10722/214731 | - |
dc.description.abstract | MiR-200 family is an important regulator of epithelial-mesenchymal transition and has been implicated in human carcinogenesis. However, their expression and functions in human cancers remain controversial. In the work presented here, we showed that miR-200 family members were frequently down-regulated in hepatocellular carcinoma (HCC). Although all five members of miR-200 family inhibited ZEB1/2 expression in HCC cell lines, we showed that overexpression only of the miR-200b/200c/429 subfamily, but not the miR-200a/141 subfamily, resulted in impeded HCC cell migration. Further investigations led to the identification of RhoA and ROCK2 as specific down-stream targets of the miR-200b/200c/429 subfamily. We demonstrated that the miR-200b/200c/429 subfamily inhibited HCC cell migration through modulating Rho/ROCK mediated cell cytoskeletal reorganization and cell-substratum adhesion. Re-expression of miR-200b significantly suppressed lung metastasis of HCC cells in an orthotopic liver implantation model in vivo. In conclusion, our findings identified the miR-200b/200c/429 subfamily as metastasis suppressor microRNAs in human HCC and highlighted the functional discrepancy among miR-200 family members. | - |
dc.language | eng | - |
dc.publisher | Impact Journals LLC. The Journal's web site is located at http://www.impactjournals.com/oncotarget/index.html | - |
dc.relation.ispartof | Oncotarget | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Cancer metastasis | - |
dc.subject | Cytoskeletal reorganization | - |
dc.subject | Hepatocellular carcinoma | - |
dc.subject | mir-200 family | - |
dc.subject | RHO/ROCK signaling pathway | - |
dc.title | MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis | - |
dc.type | Article | - |
dc.identifier.email | Wong, CM: jcmwong@hkucc.hku.hk | - |
dc.identifier.email | Tsang, FHC: fhtsang@hku.hk | - |
dc.identifier.email | Lee, JMF: joyce@pathology.hku.hk | - |
dc.identifier.email | Wong, CCL: carmencl@pathology.hku.hk | - |
dc.identifier.email | Ng, IOL: iolng@hku.hk | - |
dc.identifier.authority | Wong, CM=rp00231 | - |
dc.identifier.authority | Wong, CCL=rp01602 | - |
dc.identifier.authority | Ng, IOL=rp00335 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.18632/oncotarget.3700 | - |
dc.identifier.pmid | 25909223 | - |
dc.identifier.pmcid | PMC4537040 | - |
dc.identifier.scopus | eid_2-s2.0-84931075204 | - |
dc.identifier.hkuros | 247222 | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 15 | - |
dc.identifier.spage | 13658 | - |
dc.identifier.epage | 13670 | - |
dc.identifier.isi | WOS:000359009400058 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1949-2553 | - |