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Article: Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

TitleHuman chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1
Authors
Issue Date2015
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility and Sterility, 2015, v. 104 n. 2, p. 474-482 How to Cite?
AbstractOBJECTIVE: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro. DESIGN: Experimental study. SETTING: Reproductive biology laboratory. PATIENT(S): Healthy non-pregnant women. INTERVENTION(S): No patient interventions. MAIN OUTCOME MEASURE(S): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay. RESULT(S): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression. CONCLUSION(S): Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.
Persistent Identifierhttp://hdl.handle.net/10722/214727
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSo, KH-
dc.contributor.authorKodithuwakku, SPK-
dc.contributor.authorKottawatta, KSA-
dc.contributor.authorLi, RHW-
dc.contributor.authorChiu, PCN-
dc.contributor.authorCheung, ANY-
dc.contributor.authorNg, EHY-
dc.contributor.authorYeung, WSB-
dc.contributor.authorLee, CKF-
dc.date.accessioned2015-08-21T11:53:01Z-
dc.date.available2015-08-21T11:53:01Z-
dc.date.issued2015-
dc.identifier.citationFertility and Sterility, 2015, v. 104 n. 2, p. 474-482-
dc.identifier.issn0015-0282-
dc.identifier.urihttp://hdl.handle.net/10722/214727-
dc.description.abstractOBJECTIVE: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro. DESIGN: Experimental study. SETTING: Reproductive biology laboratory. PATIENT(S): Healthy non-pregnant women. INTERVENTION(S): No patient interventions. MAIN OUTCOME MEASURE(S): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay. RESULT(S): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression. CONCLUSION(S): Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert-
dc.relation.ispartofFertility and Sterility-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleHuman chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1-
dc.typeArticle-
dc.identifier.emailLi, RHW: raymondli@hku.hk-
dc.identifier.emailChiu, PCN: pchiucn@hku.hk-
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hk-
dc.identifier.emailNg, EHY: nghye@hku.hk-
dc.identifier.emailYeung, WSB: wsbyeung@hku.hk-
dc.identifier.emailLee, CKF: ckflee@hku.hk-
dc.identifier.authorityLi, RHW=rp01649-
dc.identifier.authorityChiu, PCN=rp00424-
dc.identifier.authorityCheung, ANY=rp00542-
dc.identifier.authorityNg, EHY=rp00426-
dc.identifier.authorityYeung, WSB=rp00331-
dc.identifier.authorityLee, CKF=rp00458-
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.fertnstert.2015.04.030-
dc.identifier.pmid25999259-
dc.identifier.scopuseid_2-s2.0-84938293127-
dc.identifier.hkuros249070-
dc.identifier.volume104-
dc.identifier.issue2-
dc.identifier.spage474-
dc.identifier.epage482-
dc.identifier.isiWOS:000363954000032-
dc.publisher.placeUnited States-

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