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Conference Paper: Micro fixture enabled in-situ imaging and manipulation of cell membrane protein

TitleMicro fixture enabled in-situ imaging and manipulation of cell membrane protein
Authors
KeywordsCell fixture
AFM
Cancer cell
Cell junction
Issue Date2010
Citation
2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, 2010, p. 165-168 How to Cite?
AbstractThis paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible porous polymer micro-mesh is designed for immobilizing living cells and protein molecules during AFM scanning. We demonstrate its usefulness by imaging cancerous B-cells as well as cell-junction molecules associated with living epithelial cells at high resolution. This technology can not only be used for imaging, but also for nanomanipulation. Current results suggest that this AFM nanomanipulation system and the novel cell fixture apparatus can be used to study the dynamic changes and function of cellular structures with unparalleled resolution both under physiological and pathophysiological conditions. ©2010 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/213128

 

DC FieldValueLanguage
dc.contributor.authorFung, Carmen Kar Man-
dc.contributor.authorYang, Ruiguo-
dc.contributor.authorLai, King Wai Chiu-
dc.contributor.authorXi, Ning-
dc.contributor.authorSeiffert-Sinha, Kristina-
dc.contributor.authorSinha, Animesh A.-
dc.date.accessioned2015-07-28T04:06:13Z-
dc.date.available2015-07-28T04:06:13Z-
dc.date.issued2010-
dc.identifier.citation2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, 2010, p. 165-168-
dc.identifier.urihttp://hdl.handle.net/10722/213128-
dc.description.abstractThis paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible porous polymer micro-mesh is designed for immobilizing living cells and protein molecules during AFM scanning. We demonstrate its usefulness by imaging cancerous B-cells as well as cell-junction molecules associated with living epithelial cells at high resolution. This technology can not only be used for imaging, but also for nanomanipulation. Current results suggest that this AFM nanomanipulation system and the novel cell fixture apparatus can be used to study the dynamic changes and function of cellular structures with unparalleled resolution both under physiological and pathophysiological conditions. ©2010 IEEE.-
dc.languageeng-
dc.relation.ispartof2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010-
dc.subjectCell fixture-
dc.subjectAFM-
dc.subjectCancer cell-
dc.subjectCell junction-
dc.titleMicro fixture enabled in-situ imaging and manipulation of cell membrane protein-
dc.typeConference_Paper-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1109/NEMS.2010.5592178-
dc.identifier.scopuseid_2-s2.0-78649240298-
dc.identifier.spage165-
dc.identifier.epage168-

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