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Conference Paper: Stiffness measurement of Burkitt's lymphoma cells with atomic force microscopy

TitleStiffness measurement of Burkitt's lymphoma cells with atomic force microscopy
Authors
KeywordsStiffness
Atomic force microscopy
Burkitt's lymphoma
Poly-L-lysine
Young's modulus
Issue Date2010
Citation
2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010, 2010 How to Cite?
AbstractCell stiffness is closely related to human health and diseases. Measuring the mechanical property of individual cells may help us to understand the mechanism of diseases at cell level and provide new methods for disease diagnosis and personalized treatment. In this paper, the poly-1-lysine was used to immobilize Burkitt's lymphoma (BL) cells onto the glass substrate for being imaged in fluid by atomic force microscopy (AFM). In nearphysiological environment, with the use of AFM, the high resolution morphology image of BL cells was obtained and the mechanical property of BL cells was measured. The detailed processes of the sample preparation and computing the Young's modulus of BL cells from force curves were presented. The experimental result indicated that the Young's modulus of BL cells fixed by glutaraldehyde was about 1MPa. These results may provide useful knowledge for understanding the mechanism of lymphoma and the application of AFM to cancer diagnosis. © 2010 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/213121

 

DC FieldValueLanguage
dc.contributor.authorLi, Mi-
dc.contributor.authorLiu, Lianqing-
dc.contributor.authorXi, Ning-
dc.contributor.authorWang, Yuebnao-
dc.contributor.authorDong, Zaili-
dc.contributor.authorXiao, Xiubin-
dc.contributor.authorZhang, Weijing-
dc.date.accessioned2015-07-28T04:06:12Z-
dc.date.available2015-07-28T04:06:12Z-
dc.date.issued2010-
dc.identifier.citation2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010, 2010-
dc.identifier.urihttp://hdl.handle.net/10722/213121-
dc.description.abstractCell stiffness is closely related to human health and diseases. Measuring the mechanical property of individual cells may help us to understand the mechanism of diseases at cell level and provide new methods for disease diagnosis and personalized treatment. In this paper, the poly-1-lysine was used to immobilize Burkitt's lymphoma (BL) cells onto the glass substrate for being imaged in fluid by atomic force microscopy (AFM). In nearphysiological environment, with the use of AFM, the high resolution morphology image of BL cells was obtained and the mechanical property of BL cells was measured. The detailed processes of the sample preparation and computing the Young's modulus of BL cells from force curves were presented. The experimental result indicated that the Young's modulus of BL cells fixed by glutaraldehyde was about 1MPa. These results may provide useful knowledge for understanding the mechanism of lymphoma and the application of AFM to cancer diagnosis. © 2010 IEEE.-
dc.languageeng-
dc.relation.ispartof2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010-
dc.subjectStiffness-
dc.subjectAtomic force microscopy-
dc.subjectBurkitt's lymphoma-
dc.subjectPoly-L-lysine-
dc.subjectYoung's modulus-
dc.titleStiffness measurement of Burkitt's lymphoma cells with atomic force microscopy-
dc.typeConference_Paper-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1109/ICBBE.2010.5515836-
dc.identifier.scopuseid_2-s2.0-77956209392-

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