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Article: IL-10 detection in murine B cells: pros and cons of the different techniques.

TitleIL-10 detection in murine B cells: pros and cons of the different techniques.
Authors
Issue Date2014
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2014, v. 1190, p. 55-69 How to Cite?
AbstractWith the recently increased understanding of the roles of B cells in immune response and autoimmune pathogenesis, various techniques have been used for the detection of IL-10 production in B cells. In this chapter, we describe several commonly used methods for the effective detection of IL-10 in B cells at both mRNA and protein levels, including quantitative PCR analysis, intracellular staining of IL-10 by flow cytometry, ELISA assay for secreted IL-10 detection, and ELISPOT assay of enumerating IL-10-producing B cells. Moreover, we provide a detailed protocol for the detection of IL-10-producing B cells in situ by immunofluorescence microscopy. Together, the application of these described methods for the detection of IL-10 will facilitate the characterization of B cell subsets with regulatory functions and enhance our current understanding on the critical roles of B cells in immune response and autoimmune development.
Persistent Identifierhttp://hdl.handle.net/10722/212120
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorLin, X-
dc.contributor.authorDeng, J-
dc.contributor.authorLu, L-
dc.date.accessioned2015-07-21T02:23:39Z-
dc.date.available2015-07-21T02:23:39Z-
dc.date.issued2014-
dc.identifier.citationMethods in Molecular Biology, 2014, v. 1190, p. 55-69-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/212120-
dc.description.abstractWith the recently increased understanding of the roles of B cells in immune response and autoimmune pathogenesis, various techniques have been used for the detection of IL-10 production in B cells. In this chapter, we describe several commonly used methods for the effective detection of IL-10 in B cells at both mRNA and protein levels, including quantitative PCR analysis, intracellular staining of IL-10 by flow cytometry, ELISA assay for secreted IL-10 detection, and ELISPOT assay of enumerating IL-10-producing B cells. Moreover, we provide a detailed protocol for the detection of IL-10-producing B cells in situ by immunofluorescence microscopy. Together, the application of these described methods for the detection of IL-10 will facilitate the characterization of B cell subsets with regulatory functions and enhance our current understanding on the critical roles of B cells in immune response and autoimmune development.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-1161-5_5-
dc.titleIL-10 detection in murine B cells: pros and cons of the different techniques.-
dc.typeArticle-
dc.identifier.emailLin, X: linxiang@hku.hk-
dc.identifier.emailDeng, J: dengjun@hku.hk-
dc.identifier.emailLu, L: liweilu@hkucc.hku.hk-
dc.identifier.authorityLu, L=rp00477-
dc.identifier.doi10.1007/978-1-4939-1161-5_5-
dc.identifier.pmid25015273-
dc.identifier.hkuros245003-
dc.identifier.volume1190-
dc.identifier.spage55-
dc.identifier.epage69-
dc.publisher.placeUnited States-

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