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Conference Paper: Ulipristal acetate may contribute to contraceptive action by its effects on tubal function

TitleUlipristal acetate may contribute to contraceptive action by its effects on tubal function
Authors
KeywordsBirth control medical sciences
Obstetrics and gynecology
Issue Date2013
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/13625187.asp
Citation
The 1st Global Conference on Contraception, Reproductive and Sexual Health, Copenhagen, Denmark, 22-25 May 2013. In The European Journal of Contraception and Reproductive Health Care, 2013, v. 18 S1, p. S193-S194, abstract P179 How to Cite?
AbstractOBJECTIVES: Ulipristal acetate (UPA), a selective progesterone receptor modulator, has been shown to be an effective drug for emergency contraception. We hypothesised that it would have modulatory effects on ciliary beat frequency and muscular contraction frequency of human fallopian tube, which might contribute to its contraceptive action. This study aimed at confirming these effects. DESIGN AND METHODS: This was an in-vitro study using human fallopian tubes obtained from six women who underwent hysterectomy for benign gynaecological conditions not involving the tubes. The tubal epithelium and smooth muscle layer of the collected tubes were stripped into small pieces. These tissue strips were cultured overnight in standardised medium containing oestradiol 100 pmol/l and progesterone 10 nmol/l resembling the physiological early luteal phase. The different tissue strips were then treated with UPA (HRA Pharma, France) at graded concentrations of 0, 20, 200 and 2000 ng/ml. These experimental concentrations were chosen based on the peak serum concentration after a pharmacological dose of UPA in human, which is around 200 ng/ml. The ciliary beat frequency of the treated tubal epithelium was measured using the photometric method. Strips of tubal smooth muscle were connected to a force transducer, and the mean contraction frequency was measured after treatment at different UPA concentrations. The different treatment groups were compared by Kruskal-Wallis test. RESULTS: We found an overall dose-dependent suppressive effect of UPA on ciliary beat frequency at all concentrations studied ( p < 0.001 for each treatment group comparing to control (0 ng/ml) and comparing among each other). Muscular contraction frequency after treatment with UPA at 20 ng/ml did not differ from control (0 ng/ml) ( p > 0.05), but was significantly reduced after treatment with UPA at 200 and 2000 ng/ml respectively ( p < 0.05). CONCLUSIONS: UPA treatment inhibits ciliary beat frequency and muscular contraction frequency of human fallopian tube at the pharmacological dose. These may contribute to post-ovulatory mechanisms for its contraceptive action.
DescriptionThis journal suppl. entitled: Book of Abstracts of the First Global Conference on Contraception, Reproductive and Sexual Health
Poster Presentation - Topic 12: New Developments and Future of Contraceptive Technology (Including Research): abstract no. P179
Persistent Identifierhttp://hdl.handle.net/10722/204895
ISSN
2015 Impact Factor: 1.236
2015 SCImago Journal Rankings: 0.576

 

DC FieldValueLanguage
dc.contributor.authorLi, HWRen_US
dc.contributor.authorLiao, Sen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorNg, EHYen_US
dc.contributor.authorO, WSen_US
dc.contributor.authorHo, PCen_US
dc.date.accessioned2014-09-20T00:56:03Z-
dc.date.available2014-09-20T00:56:03Z-
dc.date.issued2013en_US
dc.identifier.citationThe 1st Global Conference on Contraception, Reproductive and Sexual Health, Copenhagen, Denmark, 22-25 May 2013. In The European Journal of Contraception and Reproductive Health Care, 2013, v. 18 S1, p. S193-S194, abstract P179en_US
dc.identifier.issn1362-5187-
dc.identifier.urihttp://hdl.handle.net/10722/204895-
dc.descriptionThis journal suppl. entitled: Book of Abstracts of the First Global Conference on Contraception, Reproductive and Sexual Health-
dc.descriptionPoster Presentation - Topic 12: New Developments and Future of Contraceptive Technology (Including Research): abstract no. P179-
dc.description.abstractOBJECTIVES: Ulipristal acetate (UPA), a selective progesterone receptor modulator, has been shown to be an effective drug for emergency contraception. We hypothesised that it would have modulatory effects on ciliary beat frequency and muscular contraction frequency of human fallopian tube, which might contribute to its contraceptive action. This study aimed at confirming these effects. DESIGN AND METHODS: This was an in-vitro study using human fallopian tubes obtained from six women who underwent hysterectomy for benign gynaecological conditions not involving the tubes. The tubal epithelium and smooth muscle layer of the collected tubes were stripped into small pieces. These tissue strips were cultured overnight in standardised medium containing oestradiol 100 pmol/l and progesterone 10 nmol/l resembling the physiological early luteal phase. The different tissue strips were then treated with UPA (HRA Pharma, France) at graded concentrations of 0, 20, 200 and 2000 ng/ml. These experimental concentrations were chosen based on the peak serum concentration after a pharmacological dose of UPA in human, which is around 200 ng/ml. The ciliary beat frequency of the treated tubal epithelium was measured using the photometric method. Strips of tubal smooth muscle were connected to a force transducer, and the mean contraction frequency was measured after treatment at different UPA concentrations. The different treatment groups were compared by Kruskal-Wallis test. RESULTS: We found an overall dose-dependent suppressive effect of UPA on ciliary beat frequency at all concentrations studied ( p < 0.001 for each treatment group comparing to control (0 ng/ml) and comparing among each other). Muscular contraction frequency after treatment with UPA at 20 ng/ml did not differ from control (0 ng/ml) ( p > 0.05), but was significantly reduced after treatment with UPA at 200 and 2000 ng/ml respectively ( p < 0.05). CONCLUSIONS: UPA treatment inhibits ciliary beat frequency and muscular contraction frequency of human fallopian tube at the pharmacological dose. These may contribute to post-ovulatory mechanisms for its contraceptive action.-
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/13625187.asp-
dc.relation.ispartofThe European Journal of Contraception and Reproductive Health Careen_US
dc.rightsThe European Journal of Contraception and Reproductive Health Care. Copyright © Informa Healthcare.-
dc.subjectBirth control medical sciences-
dc.subjectObstetrics and gynecology-
dc.titleUlipristal acetate may contribute to contraceptive action by its effects on tubal functionen_US
dc.typeConference_Paperen_US
dc.identifier.emailLi, HWR: raymondli@hku.hken_US
dc.identifier.emailLiao, S: subin@hkucc.hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_US
dc.identifier.emailNg, EHY: nghye@hku.hken_US
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_US
dc.identifier.emailHo, PC: pcho@hku.hken_US
dc.identifier.authorityLi, HWR=rp01649en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3109/13625187.2013.793038-
dc.identifier.hkuros214821en_US
dc.identifier.hkuros239085-
dc.identifier.volume18-
dc.identifier.issueS1-
dc.identifier.spageS193-
dc.identifier.epageS194-
dc.publisher.placeUnited Kingdom-
dc.customcontrol.immutablesml 141120-

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