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Conference Paper: Type IIA Procollagen as a Modulator of Nodal Signaling

TitleType IIA Procollagen as a Modulator of Nodal Signaling
Authors
Issue Date2013
Citation
The 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013 How to Cite?
AbstractEmbryonic development depends on the provision of morphogenetic instructions which are generated by signaling factors such as those of the transforming growth factor-beta (TGF-β) superfamily, that cells receive from their neighbours. These instructions contribute towards the determination of cell lineage and differentiation, tissue growth and tissue patterning in the primary body axes. There is increasing evidence that components of the extracellular matrix (ECM) plays a role in modulating signaling activity and morphogen gradients by the sequestration and activation of growth factors. Nodal is a member of the TGFβ family that plays crucial roles in mesendoderm formation and left-right patterning during early vertebrate development. Type IIA procollagen (IIA) is an isoform of type II procollagen, an ECM protein that has been demonstrated to bind to BMPs 2, 4, and 7 and TGFβ1 in vitro and was reputed to antagonize BMP signaling in Xenopus embryos. It has been proposed that IIA may regulate early embryonic patterning as a regulator either by facilitation or by inhibition via binding to growth factors or signaling molecules. Here we show that IIA may act as a facilitator of nodal signaling. In mice lacking IIA we found the expression of Nodal and its signaling downstream targets such as Nodal itself and Pitx2 were either altered or dramatically reduced in the node and left lateral plate mesoderm (LPM). To understand the role of IIA in nodal signaling, we tested for direct interaction between IIA and nodal. By in vitro transfection assays and co-immunoprecipitation assays, we have found that IIA can bind nodal and is a facilitator of nodal signaling. Our results reveal a potential role for IIA in regulating embryonic patterning by facilitating Nodal signaling.
DescriptionConference Theme: The Extracellular Matrix Niche
Oral Presentation
Session 1: The matrix niche
The Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf
Persistent Identifierhttp://hdl.handle.net/10722/203885

 

DC FieldValueLanguage
dc.contributor.authorCheah, KSEen_US
dc.contributor.authorGao, Yen_US
dc.contributor.authorWong, Sen_US
dc.contributor.authorShen, MMen_US
dc.contributor.authorChan, Den_US
dc.date.accessioned2014-09-19T16:43:06Z-
dc.date.available2014-09-19T16:43:06Z-
dc.date.issued2013en_US
dc.identifier.citationThe 9th Pan Pacific Connective Tissue Societies Symposium (PPCTSS 2013), Hong Kong, China, 24-27 November 2013. In the Program Book of the 9th Pan Pacific Connective Tissue Societies Symposium, 2013en_US
dc.identifier.urihttp://hdl.handle.net/10722/203885-
dc.descriptionConference Theme: The Extracellular Matrix Niche-
dc.descriptionOral Presentation-
dc.descriptionSession 1: The matrix niche-
dc.descriptionThe Program can be viewed at: http://ppctss2013.org/Online%20Program%20Book.pdf-
dc.description.abstractEmbryonic development depends on the provision of morphogenetic instructions which are generated by signaling factors such as those of the transforming growth factor-beta (TGF-β) superfamily, that cells receive from their neighbours. These instructions contribute towards the determination of cell lineage and differentiation, tissue growth and tissue patterning in the primary body axes. There is increasing evidence that components of the extracellular matrix (ECM) plays a role in modulating signaling activity and morphogen gradients by the sequestration and activation of growth factors. Nodal is a member of the TGFβ family that plays crucial roles in mesendoderm formation and left-right patterning during early vertebrate development. Type IIA procollagen (IIA) is an isoform of type II procollagen, an ECM protein that has been demonstrated to bind to BMPs 2, 4, and 7 and TGFβ1 in vitro and was reputed to antagonize BMP signaling in Xenopus embryos. It has been proposed that IIA may regulate early embryonic patterning as a regulator either by facilitation or by inhibition via binding to growth factors or signaling molecules. Here we show that IIA may act as a facilitator of nodal signaling. In mice lacking IIA we found the expression of Nodal and its signaling downstream targets such as Nodal itself and Pitx2 were either altered or dramatically reduced in the node and left lateral plate mesoderm (LPM). To understand the role of IIA in nodal signaling, we tested for direct interaction between IIA and nodal. By in vitro transfection assays and co-immunoprecipitation assays, we have found that IIA can bind nodal and is a facilitator of nodal signaling. Our results reveal a potential role for IIA in regulating embryonic patterning by facilitating Nodal signaling.-
dc.languageengen_US
dc.relation.ispartofPan Pacific Connective Tissue Societies Symposium (PPCTSS)en_US
dc.titleType IIA Procollagen as a Modulator of Nodal Signalingen_US
dc.typeConference_Paperen_US
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hken_US
dc.identifier.emailGao, Y: genegao@hku.hken_US
dc.identifier.emailChan, D: chand@hku.hken_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.identifier.authorityChan, D=rp00540en_US
dc.identifier.hkuros240450en_US

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