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Conference Paper: Nerve guidance channel constructed by Schwann cell seeded aligned chitosan nanofiber

TitleNerve guidance channel constructed by Schwann cell seeded aligned chitosan nanofiber
Authors
Issue Date2014
PublisherBlackwell Publishing Ltd.
Citation
The 12th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Kaohsiung, Taiwan, 23–26 August 2014. In Journal of Neurochemistry, 2014, v. 130 n. Suppl. 1, p. 49, abstract no. P01-11 How to Cite?
AbstractSchwann cell-seeded guidance channels have been exploited to bridge and guide axonal re-growth across gaps in lesioned nerves. By orienting the Schwann cell growth on aligned nanofibers, we hypothesized that axonal growth can be guided along the designated direction towards the target. Chitosan was the choice scaffold material given its biocompatibility and the tunable susceptibility to biodegradation. Chitosan was dissolved in trifluoroacetic acid/methylene chloride solution and was electrospun onto a high speed rotating drum. Stability of the chitosan fibers in aqueous, physiological environment was achieved with the use of sodium carbonate to neutralize residual acidity in the chitosan fiber preparation. Schwann cells seeded onto these stabilized aligned chitosan nanofibers aligned uniaxially with the chitosan nanofibers. In addition, by seeding dissociated cells of dorsal root ganglia (DRG, E14/15 rats) onto the uniaxially aligned nanofibers, both neurons and Schwann cells were aligned with the uniaxial arrangement of nanofibers. The Schwann cells could be induced to myelinate neurites extending from neuronal cell bodies. The Schwann cell-seeded nanofiber was rolled into a model of nerve conduit. The Schwann cell aligned along the longitudinal axis of the conduit. These in vitro results provide proof of principle for pursuing improvement in post-traumatic recovery from nerve injury with use of Schwann cell-seeded uniaxially aligned chitosan nanofibers as a nerve guidance channel. (Supported by ITS/100/ 10 of the Innovation and Technology Commission, HK Government).
DescriptionPoster Presentation 1
Persistent Identifierhttp://hdl.handle.net/10722/203812
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorTung, WTen_US
dc.contributor.authorTam, KWen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-09-19T16:41:12Z-
dc.date.available2014-09-19T16:41:12Z-
dc.date.issued2014en_US
dc.identifier.citationThe 12th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Kaohsiung, Taiwan, 23–26 August 2014. In Journal of Neurochemistry, 2014, v. 130 n. Suppl. 1, p. 49, abstract no. P01-11en_US
dc.identifier.issn0022-3042-
dc.identifier.urihttp://hdl.handle.net/10722/203812-
dc.descriptionPoster Presentation 1-
dc.description.abstractSchwann cell-seeded guidance channels have been exploited to bridge and guide axonal re-growth across gaps in lesioned nerves. By orienting the Schwann cell growth on aligned nanofibers, we hypothesized that axonal growth can be guided along the designated direction towards the target. Chitosan was the choice scaffold material given its biocompatibility and the tunable susceptibility to biodegradation. Chitosan was dissolved in trifluoroacetic acid/methylene chloride solution and was electrospun onto a high speed rotating drum. Stability of the chitosan fibers in aqueous, physiological environment was achieved with the use of sodium carbonate to neutralize residual acidity in the chitosan fiber preparation. Schwann cells seeded onto these stabilized aligned chitosan nanofibers aligned uniaxially with the chitosan nanofibers. In addition, by seeding dissociated cells of dorsal root ganglia (DRG, E14/15 rats) onto the uniaxially aligned nanofibers, both neurons and Schwann cells were aligned with the uniaxial arrangement of nanofibers. The Schwann cells could be induced to myelinate neurites extending from neuronal cell bodies. The Schwann cell-seeded nanofiber was rolled into a model of nerve conduit. The Schwann cell aligned along the longitudinal axis of the conduit. These in vitro results provide proof of principle for pursuing improvement in post-traumatic recovery from nerve injury with use of Schwann cell-seeded uniaxially aligned chitosan nanofibers as a nerve guidance channel. (Supported by ITS/100/ 10 of the Innovation and Technology Commission, HK Government).-
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd.-
dc.relation.ispartofJournal of Neurochemistryen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.titleNerve guidance channel constructed by Schwann cell seeded aligned chitosan nanofiberen_US
dc.typeConference_Paperen_US
dc.identifier.emailTam, KW: antam@graduate.hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/jnc.12778-
dc.identifier.hkuros238261en_US
dc.identifier.volume130-
dc.identifier.issueSuppl. 1-
dc.identifier.spage49, abstract no. P01-11-
dc.identifier.epage49, abstract no. P01-11-
dc.publisher.placeUnited Kingdom-

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