File Download
Supplementary

Conference Paper: Dynamics of chondroitin sulfotransferase expression for cranial motor neuron migration in rat hindbrain development

TitleDynamics of chondroitin sulfotransferase expression for cranial motor neuron migration in rat hindbrain development
Authors
Issue Date2014
PublisherFederation of European Neuroscience Societies.
Citation
The 9th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS), Milan, Italy, 5-9 July 2014. How to Cite?
AbstractThe proper positioning of neurons for the establishment of functional connectivity of defined neural circuits relies on neuronal migration. We and others reported the restrictive role of chondroitin sulphate (CS) moieties of proteoglycans in axonal fasciculation. CS moieties are therefore hypothesized to regulate the timely orchestration of cranial motor neuron migration during hindbrain development by the differential sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. Hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture for time lapse video recording of individual neuronal somal movements. In control cultures, we observed the advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal somata lost the direction movement but retained the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environments surrounding the migrating neuronal somata. Chondroitin-4-sulfotransferases 2 (C4ST2) showed relatively abundant mRNA expression among cells heading away from the core whereas chondroitin-4-sulfotransferase 1 (C4ST1) mRNA was found essentially in the centre of the explant by in situ hybridization. Thus far, the results suggest varying sulfation of chondroitins on proteoglycans expressed by neurons in determining the migratory phenotype.
DescriptionPoster Session A21 - Transplantation and regeneration - Regeneration: abstract no. FENS-1040
Persistent Identifierhttp://hdl.handle.net/10722/203810

 

DC FieldValueLanguage
dc.contributor.authorWong, MKen_US
dc.contributor.authorLi, Men_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-09-19T16:41:11Z-
dc.date.available2014-09-19T16:41:11Z-
dc.date.issued2014en_US
dc.identifier.citationThe 9th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS), Milan, Italy, 5-9 July 2014.en_US
dc.identifier.urihttp://hdl.handle.net/10722/203810-
dc.descriptionPoster Session A21 - Transplantation and regeneration - Regeneration: abstract no. FENS-1040-
dc.description.abstractThe proper positioning of neurons for the establishment of functional connectivity of defined neural circuits relies on neuronal migration. We and others reported the restrictive role of chondroitin sulphate (CS) moieties of proteoglycans in axonal fasciculation. CS moieties are therefore hypothesized to regulate the timely orchestration of cranial motor neuron migration during hindbrain development by the differential sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. Hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture for time lapse video recording of individual neuronal somal movements. In control cultures, we observed the advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal somata lost the direction movement but retained the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environments surrounding the migrating neuronal somata. Chondroitin-4-sulfotransferases 2 (C4ST2) showed relatively abundant mRNA expression among cells heading away from the core whereas chondroitin-4-sulfotransferase 1 (C4ST1) mRNA was found essentially in the centre of the explant by in situ hybridization. Thus far, the results suggest varying sulfation of chondroitins on proteoglycans expressed by neurons in determining the migratory phenotype.-
dc.languageengen_US
dc.publisherFederation of European Neuroscience Societies.-
dc.relation.ispartof9th FENS Forum of Neuroscience 2014en_US
dc.titleDynamics of chondroitin sulfotransferase expression for cranial motor neuron migration in rat hindbrain developmenten_US
dc.typeConference_Paperen_US
dc.identifier.emailLi, M: limeihk@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros238254en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats