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Conference Paper: Chondroitin sulfotransferase expression pattern in migrating motor neurons of the rat embryonic hindbrain

TitleChondroitin sulfotransferase expression pattern in migrating motor neurons of the rat embryonic hindbrain
Authors
KeywordsMigration
Hindbrain
Proteoglycan
Issue Date2013
PublisherSociety for Neuroscience.
Citation
The 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013 How to Cite?
AbstractChondroitin sulfate (CS) moieties of proteoglycans are extracellular matrix components that have been implicated in the timing and patterning of axon fasciculation during embryonic brain development. It, nonetheless, remains unclear if these moieties can also control neuronal soma migration in the developmental process. We hypothesized that the cranial motor neuron migration in the hindbrain is modulated by varying sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. In this project, hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture. In control cultures, time lapse video microscopy revealed advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal cell bodies lost the directional movement but not the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environment surrounding the migrating neuronal cell bodies. In situ hybridization revealed the relatively abundant expression of chondroitin-4-sulfotransferase 2 (C4ST2) mRNA among cells heading away from the explant core. Taken together, the present results showed that CS moieties expressed by migrating neurons differ in sulfation pattern from those in the vicinity of non-migrating neurons.
DescriptionPoster session 219: Patterning of Spinal Cord, Brain Stem, and Cerebellum
Program#219.07 & Poster#A7
Fulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=29ae9caa-c4a1-4a29-988d-7c597965945e&cKey=4fd5a5f0-2df1-494c-b999-e40ae154eac0&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf
Persistent Identifierhttp://hdl.handle.net/10722/203791

 

DC FieldValueLanguage
dc.contributor.authorWong, MKen_US
dc.contributor.authorLi, Men_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-09-19T16:41:05Z-
dc.date.available2014-09-19T16:41:05Z-
dc.date.issued2013en_US
dc.identifier.citationThe 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013en_US
dc.identifier.urihttp://hdl.handle.net/10722/203791-
dc.descriptionPoster session 219: Patterning of Spinal Cord, Brain Stem, and Cerebellum-
dc.descriptionProgram#219.07 & Poster#A7-
dc.descriptionFulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=29ae9caa-c4a1-4a29-988d-7c597965945e&cKey=4fd5a5f0-2df1-494c-b999-e40ae154eac0&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf-
dc.description.abstractChondroitin sulfate (CS) moieties of proteoglycans are extracellular matrix components that have been implicated in the timing and patterning of axon fasciculation during embryonic brain development. It, nonetheless, remains unclear if these moieties can also control neuronal soma migration in the developmental process. We hypothesized that the cranial motor neuron migration in the hindbrain is modulated by varying sulfation patterns of the chondroitins between the migrating and ready-to-migrate neurons. In this project, hindbrain explants of E11.5 Sprague Dawley rats were maintained in culture. In control cultures, time lapse video microscopy revealed advancement of neuronal cell bodies in the direction of the leading process away from the explant core. In test cultures treated with chondroitinase ABC, the neuronal cell bodies lost the directional movement but not the motility. Immunocytochemistry confirmed the presence of CS56 epitopes among Tuj-1-positive neurons not only in the explant core and those advancing beyond the core, but also in the environment surrounding the migrating neuronal cell bodies. In situ hybridization revealed the relatively abundant expression of chondroitin-4-sulfotransferase 2 (C4ST2) mRNA among cells heading away from the explant core. Taken together, the present results showed that CS moieties expressed by migrating neurons differ in sulfation pattern from those in the vicinity of non-migrating neurons.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2013en_US
dc.rightsNeuroscience 2013. Copyright © Society for Neuroscience.-
dc.subjectMigration-
dc.subjectHindbrain-
dc.subjectProteoglycan-
dc.titleChondroitin sulfotransferase expression pattern in migrating motor neurons of the rat embryonic hindbrainen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, MK: wongmmk@hku.hken_US
dc.identifier.emailLi, M: limeihk@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.identifier.hkuros238207en_US
dc.publisher.placeUnited States-

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