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Conference Paper: Loops, hairpins and flipped bases: a DNA aptamer that discriminates Plasmodium lactate dehydrogenase from the blind watchmaker

TitleLoops, hairpins and flipped bases: a DNA aptamer that discriminates Plasmodium lactate dehydrogenase from the blind watchmaker
Authors
Issue Date2013
PublisherOligonucleotide Therapeutics Society (OTS).
Citation
The 9th Annual Meeting of the Oligonucleotide Therapeutics Society (OTS 2013), Naples, Italy, 6-8 October 2013. In Program Book, 2013, p. 187 How to Cite?
AbstractLittle is known about how aptamers achieve their specificities in binding and discriminating between closely related targets. Under the pretext of investigating the potential for aptamers in malaria diagnostics, here we solve the crystal structure of a new DNA aptamer which was selected and evolved to bind specifically to the Plasmodium falciparum lactate dehydrogenase (PfLDH) and not bind to human lactate dehydrogenase. The structure reveals two aptamers bind per Plasmodium lactate dehydrogenase tetramer with opposite apical geometry, whereby each aptamer has a distorted hairpin structure. The aptamer comprises a B-helix stem, an asymmetric internal loop involved in target discrimination and an apical loop involved in binding interactions. Each loop contains a critical flipped base. Isothermal titration calorimetry, surface plasmon resonance and electrophoretic mobility shift assay all provide evidence for binding with a dissociation constant in the range 40-70 …
DescriptionThe Oligo Meeting
Poster Presentation: no. 113
Persistent Identifierhttp://hdl.handle.net/10722/203680

 

DC FieldValueLanguage
dc.contributor.authorTanner, Jen_US
dc.contributor.authorCheung, YWen_US
dc.contributor.authorWatt, RMen_US
dc.contributor.authorKotaka, Men_US
dc.date.accessioned2014-09-19T15:58:23Z-
dc.date.available2014-09-19T15:58:23Z-
dc.date.issued2013en_US
dc.identifier.citationThe 9th Annual Meeting of the Oligonucleotide Therapeutics Society (OTS 2013), Naples, Italy, 6-8 October 2013. In Program Book, 2013, p. 187en_US
dc.identifier.urihttp://hdl.handle.net/10722/203680-
dc.descriptionThe Oligo Meeting-
dc.descriptionPoster Presentation: no. 113-
dc.description.abstractLittle is known about how aptamers achieve their specificities in binding and discriminating between closely related targets. Under the pretext of investigating the potential for aptamers in malaria diagnostics, here we solve the crystal structure of a new DNA aptamer which was selected and evolved to bind specifically to the Plasmodium falciparum lactate dehydrogenase (PfLDH) and not bind to human lactate dehydrogenase. The structure reveals two aptamers bind per Plasmodium lactate dehydrogenase tetramer with opposite apical geometry, whereby each aptamer has a distorted hairpin structure. The aptamer comprises a B-helix stem, an asymmetric internal loop involved in target discrimination and an apical loop involved in binding interactions. Each loop contains a critical flipped base. Isothermal titration calorimetry, surface plasmon resonance and electrophoretic mobility shift assay all provide evidence for binding with a dissociation constant in the range 40-70 …-
dc.languageengen_US
dc.publisherOligonucleotide Therapeutics Society (OTS).-
dc.relation.ispartofAnnual Meeting of the Oligonucleotide Therapeutics Society, OTS 2013en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleLoops, hairpins and flipped bases: a DNA aptamer that discriminates Plasmodium lactate dehydrogenase from the blind watchmakeren_US
dc.typeConference_Paperen_US
dc.identifier.emailTanner, J: jatanner@hku.hken_US
dc.identifier.emailCheung, YW: cheungw@hkucc.hku.hken_US
dc.identifier.emailWatt, RM: rmwatt@hku.hken_US
dc.identifier.emailKotaka, M: masayo@hku.hken_US
dc.identifier.authorityTanner, J=rp00495en_US
dc.identifier.authorityWatt, RM=rp00043en_US
dc.identifier.authorityKotaka, M=rp00293en_US
dc.description.naturepostprint-
dc.identifier.hkuros238511en_US
dc.identifier.spage187-
dc.identifier.epage187-
dc.publisher.placeItaly-

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