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Article: Supported membrane formation, characterization, functionalization, and patterning for application in biological science and technology

TitleSupported membrane formation, characterization, functionalization, and patterning for application in biological science and technology
Authors
KeywordsFluorescence recovery after photobleaching (FRAP)
Membrane functionalization
Photolithography
Quantitative fluorescence measurement
Small unilamellar vesicle (SUV)
Issue Date2010
PublisherJohn Wiley & Sons, Inc..
Citation
Current Protocols in Chemical Biology, 2010, v. 2 n. 4, p. 235-269 How to Cite?
AbstractSupported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein-protein and cell-cell interaction, to study the molecular interactions at interfaces and the heterogeneities of plasma membranes. The advantages of a supported membrane system include the ability to control membrane composition and the compatibility it has with various surface-sensitive microscopic and spectroscopic techniques. Recent advances in micro- and nanotechnology have greatly extended the use of supported membranes to address key questions in cell biology. Although supported membranes can be easily made by vesicle fusion, the samples need careful preparation for this process to be efficient. The protocols in this unit comprehensively describe procedures to prepare, functionalize, and characterize supported membranes.
Persistent Identifierhttp://hdl.handle.net/10722/202259
ISSN
2015 SCImago Journal Rankings: 0.723

 

DC FieldValueLanguage
dc.contributor.authorLin, WC-
dc.contributor.authorYu, C-
dc.contributor.authorTriffo, S-
dc.contributor.authorGroves, JT-
dc.date.accessioned2014-09-01T01:57:03Z-
dc.date.available2014-09-01T01:57:03Z-
dc.date.issued2010-
dc.identifier.citationCurrent Protocols in Chemical Biology, 2010, v. 2 n. 4, p. 235-269-
dc.identifier.issn2160-4762-
dc.identifier.urihttp://hdl.handle.net/10722/202259-
dc.description.abstractSupported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein-protein and cell-cell interaction, to study the molecular interactions at interfaces and the heterogeneities of plasma membranes. The advantages of a supported membrane system include the ability to control membrane composition and the compatibility it has with various surface-sensitive microscopic and spectroscopic techniques. Recent advances in micro- and nanotechnology have greatly extended the use of supported membranes to address key questions in cell biology. Although supported membranes can be easily made by vesicle fusion, the samples need careful preparation for this process to be efficient. The protocols in this unit comprehensively describe procedures to prepare, functionalize, and characterize supported membranes.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc..-
dc.relation.ispartofCurrent Protocols in Chemical Biology-
dc.rightsCurrent Protocols in Chemical Biology. Copyright © John Wiley & Sons, Inc..-
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.subjectFluorescence recovery after photobleaching (FRAP)-
dc.subjectMembrane functionalization-
dc.subjectPhotolithography-
dc.subjectQuantitative fluorescence measurement-
dc.subjectSmall unilamellar vesicle (SUV)-
dc.titleSupported membrane formation, characterization, functionalization, and patterning for application in biological science and technologyen_US
dc.typeArticleen_US
dc.identifier.emailYu, C: chyu1@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/9780470559277.ch100131-
dc.identifier.pmid23839978-
dc.identifier.volume2-
dc.identifier.issue4-
dc.identifier.spage235-
dc.identifier.epage269-
dc.publisher.placeUnited States-

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