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Conference Paper: Chondroitinase ABC-I and -II crosslinked chitosan beads improve axonal regrowth in CSPG-enriched astrocyte culture

TitleChondroitinase ABC-I and -II crosslinked chitosan beads improve axonal regrowth in CSPG-enriched astrocyte culture
Authors
Issue Date2014
Citation
The 9th International Symposium on Healthy Aging (ISHA 2014), Hong Kong, 8-9 March 2014. How to Cite?
AbstractAfter spinal cord injury, axonal regrowth is often restricted due to upregulation of chondroitin sulfate proteoglycans (CSPGs) at the lesion site. Chondroitinase ABC I and II (recombinant ChABC-I & II) cleave CS moieties of the PGs enhancing prospects of axonal regrowth through the lesion. To reduce decay of ChABC activity in vivo, we attempted to separately immobilize ChABC-I or -II on chitosan beads using glutaraldehyde as a crosslinker. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in astrocyte cultures that had been activated by transforming growth factor beta(TGF-β) to secrete CSPGs. Neurite length was increased in co-cultures of TGF-β activated astrocytes and cortical neurons mixed with immobilized ChABC-I, immobilized ChABC-II or both. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture.
DescriptionSymposium Theme: Aging with Confidence
Persistent Identifierhttp://hdl.handle.net/10722/201176

 

DC FieldValueLanguage
dc.contributor.authorKwok, LFen_US
dc.contributor.authorTam, KWen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-08-21T07:16:31Z-
dc.date.available2014-08-21T07:16:31Z-
dc.date.issued2014en_US
dc.identifier.citationThe 9th International Symposium on Healthy Aging (ISHA 2014), Hong Kong, 8-9 March 2014.en_US
dc.identifier.urihttp://hdl.handle.net/10722/201176-
dc.descriptionSymposium Theme: Aging with Confidence-
dc.description.abstractAfter spinal cord injury, axonal regrowth is often restricted due to upregulation of chondroitin sulfate proteoglycans (CSPGs) at the lesion site. Chondroitinase ABC I and II (recombinant ChABC-I & II) cleave CS moieties of the PGs enhancing prospects of axonal regrowth through the lesion. To reduce decay of ChABC activity in vivo, we attempted to separately immobilize ChABC-I or -II on chitosan beads using glutaraldehyde as a crosslinker. Immobilized ChABC-I demonstrated CS-cleaving activity both in biochemical assay and in astrocyte cultures that had been activated by transforming growth factor beta(TGF-β) to secrete CSPGs. Neurite length was increased in co-cultures of TGF-β activated astrocytes and cortical neurons mixed with immobilized ChABC-I, immobilized ChABC-II or both. Given CSPG enrichment at astrocyte-Schwann cell (A/S) encounters, A/S confrontation co-culture was used in a further bioassay of immobilized enzyme activity. Preliminary data showed that neurite length was increased in ChABC-I treated A/S co-culture. In future, the effect of immobilized ChABC-I and -II on neurite length will be tested in A/S co-culture.en_US
dc.languageengen_US
dc.relation.ispartofAbstracts of 9th ISHA 2014: Aging with Confidence: 73en_US
dc.titleChondroitinase ABC-I and -II crosslinked chitosan beads improve axonal regrowth in CSPG-enriched astrocyte cultureen_US
dc.typeConference_Paperen_US
dc.identifier.emailKwok, LF: lamfungs@hku.hken_US
dc.identifier.emailTam, KW: kwtam@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.hkuros234864en_US
dc.identifier.hkuros238328-

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