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Conference Paper: Roles of Neuregulin 1 Type III in the Ex Vivo Generation of Fate Committed Schwann cells from Bone Marrow Stromal Cells

TitleRoles of Neuregulin 1 Type III in the Ex Vivo Generation of Fate Committed Schwann cells from Bone Marrow Stromal Cells
Authors
Issue Date2013
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090
Citation
The 11th European Meeting on Glial Cell Function in Health and Disease (GLIA Meeting 2013), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 suppl. 1, p. S97, abstract no. T06-02B How to Cite?
AbstractSchwann cell transplantation accelerates functional recovery from nerve injury by promoting axonal regrowth and remyelination. Our group had generated fate committed Schwann cells from bone marrow stromal cells (Shea et. al, 2010). It was also demonstrated that co-culture with dorsal root ganglia (DRG, E14/15 rats) neurons was essential for the fate commitment of bone marrow derived Schwann cell-like cells (SCLCs) (Shea et. al, 2010). Signals from the DRG neurons are therefore thought to direct the switch of SCLCs to fate committed Schwann cells. The reliance on DRG neuron co-culture for SCLCs fate commitment stands as a major hurdle for the therapeutic application of this finding. Thus, we aim to unravel the mechanisms underlying SCLCs fate commitment in order to develop a DRG neuron-free condition for generating fate committed Schwann cells. We hypothesised that the switch is brought about by the membrane bound neuregulin (NRG), NRG 1 Type III, but not the other soluble isoforms of neuregulin. As our results show that SCLCs treated with soluble neuregulin did not show significant changes in morphology nor marker expression when compared with untreated SCLCs. NRG 1 Type III expression was observed on purified DRG neurons by immunocytochemistry, Western blot analysis as well as reverse transcription PCR for the mRNA. Mammalian expression constructs for NRG 1 Type III were made by transfection into human embryonic kidney cells, HEK 293T, and mouse embryonic fibroblasts (MEF) with the aim of generating surrogate cell types for co-culture with SCLCs to pursue cell-specific effects of NRG 1 Type III on differentiation of SCLCs. The findings promise a way for generating fate committed Schwann cells for autologous transplantation for recovery after nerve injury.
DescriptionPoster Presentation
Topic: T6 Glial-neuronal interactions
Persistent Identifierhttp://hdl.handle.net/10722/201173
ISSN
2015 Impact Factor: 5.997
2015 SCImago Journal Rankings: 3.296

 

DC FieldValueLanguage
dc.contributor.authorLeung, HYen_US
dc.contributor.authorTsui, YPen_US
dc.contributor.authorShea, GKHen_US
dc.contributor.authorTai, WYEen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2014-08-21T07:16:30Z-
dc.date.available2014-08-21T07:16:30Z-
dc.date.issued2013en_US
dc.identifier.citationThe 11th European Meeting on Glial Cell Function in Health and Disease (GLIA Meeting 2013), Berlin, Germany, 3-6 July 2013. In Glia, 2013, v. 61 suppl. 1, p. S97, abstract no. T06-02Ben_US
dc.identifier.issn0894-1491-
dc.identifier.urihttp://hdl.handle.net/10722/201173-
dc.descriptionPoster Presentation-
dc.descriptionTopic: T6 Glial-neuronal interactions-
dc.description.abstractSchwann cell transplantation accelerates functional recovery from nerve injury by promoting axonal regrowth and remyelination. Our group had generated fate committed Schwann cells from bone marrow stromal cells (Shea et. al, 2010). It was also demonstrated that co-culture with dorsal root ganglia (DRG, E14/15 rats) neurons was essential for the fate commitment of bone marrow derived Schwann cell-like cells (SCLCs) (Shea et. al, 2010). Signals from the DRG neurons are therefore thought to direct the switch of SCLCs to fate committed Schwann cells. The reliance on DRG neuron co-culture for SCLCs fate commitment stands as a major hurdle for the therapeutic application of this finding. Thus, we aim to unravel the mechanisms underlying SCLCs fate commitment in order to develop a DRG neuron-free condition for generating fate committed Schwann cells. We hypothesised that the switch is brought about by the membrane bound neuregulin (NRG), NRG 1 Type III, but not the other soluble isoforms of neuregulin. As our results show that SCLCs treated with soluble neuregulin did not show significant changes in morphology nor marker expression when compared with untreated SCLCs. NRG 1 Type III expression was observed on purified DRG neurons by immunocytochemistry, Western blot analysis as well as reverse transcription PCR for the mRNA. Mammalian expression constructs for NRG 1 Type III were made by transfection into human embryonic kidney cells, HEK 293T, and mouse embryonic fibroblasts (MEF) with the aim of generating surrogate cell types for co-culture with SCLCs to pursue cell-specific effects of NRG 1 Type III on differentiation of SCLCs. The findings promise a way for generating fate committed Schwann cells for autologous transplantation for recovery after nerve injury.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090-
dc.relation.ispartofGliaen_US
dc.rightsGlia. Copyright © John Wiley & Sons, Inc.-
dc.titleRoles of Neuregulin 1 Type III in the Ex Vivo Generation of Fate Committed Schwann cells from Bone Marrow Stromal Cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, HY: echokath@hku.hken_US
dc.identifier.emailTsui, YP: alex2013@hku.hken_US
dc.identifier.emailShea, GKH: gkshea@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityShea, GKH=rp01781en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.doi10.1002/glia.22530-
dc.identifier.hkuros234625en_US
dc.identifier.hkuros221783-
dc.identifier.hkuros232496-
dc.identifier.hkuros238148-
dc.identifier.volume61-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS97, abstract no. T06-02B-
dc.identifier.epageS97, abstract no. T06-02B-
dc.publisher.placeUnited States-

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