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Article: Th17 cells play a critical role in the development of experimental Sjogren's syndrome

TitleTh17 cells play a critical role in the development of experimental Sjogren's syndrome
Authors
Issue Date2015
PublisherBMJ Publishing Group. The Journal's web site is located at http://ard.bmjjournals.com/
Citation
Annals of the Rheumatic Diseases, 2015, v. 74 n. 6, p. 1302-1310 How to Cite?
AbstractObjective Although Th17 cells have been increasingly recognised as an important effector in various autoimmune diseases, their function in the pathogenesis of Sjögren's syndrome (SS) remains largely uncharacterised. This study aims to determine the role of Th17 cells in the development of experimental SS (ESS). Methods The ESS was induced in wildtype and IL-17A knockout (IL-17 KO) C57BL/6 mice immunised with salivary glands (SG) proteins. Phenotypic analysis of immune cells in the draining cervical lymph nodes (CLN) and SG was performed by flow cytometry and immunofluorescence microscopy. To determine the role of Th17 cells in ESS, immunised IL-17 KO mice were adoptively transferred with in vitro-generated Th17 cells and monitored for SS development. The salivary flow rate was measured, whereas inflammatory infiltration and tissue destruction in SG were assessed by histopathology. Results SG protein-immunised mice developed overt SS symptoms with increased Th17 cells detected in CLN and within lymphocytic foci in inflamed SG. Notably, immunised IL-17 KO mice were completely resistant for SS induction, showing no evidence of disease symptoms and histopathological changes in SG. Adoptive transfer of Th17 cells rapidly induced the onset of ESS in immunised IL-17 KO mice with markedly reduced saliva secretion, elevated autoantibody production and pronounced inflammation and tissue damage in SG. Conclusions Our findings have defined a critical role of Th17 cells in the pathogenesis of ESS. Further studies may validate Th17 cell as a potential target for treating SS.
Persistent Identifierhttp://hdl.handle.net/10722/200704
ISSN
2015 Impact Factor: 12.384
2015 SCImago Journal Rankings: 4.537

 

DC FieldValueLanguage
dc.contributor.authorLin, X-
dc.contributor.authorRui, K-
dc.contributor.authorDeng, J-
dc.contributor.authorTian, J-
dc.contributor.authorWang, X-
dc.contributor.authorWang, S-
dc.contributor.authorKo, KH-
dc.contributor.authorJiao, Z-
dc.contributor.authorChan, VSF-
dc.contributor.authorLau, WCS-
dc.contributor.authorCao, X-
dc.contributor.authorLu, L-
dc.date.accessioned2014-08-21T06:55:41Z-
dc.date.available2014-08-21T06:55:41Z-
dc.date.issued2015-
dc.identifier.citationAnnals of the Rheumatic Diseases, 2015, v. 74 n. 6, p. 1302-1310-
dc.identifier.issn0003-4967-
dc.identifier.urihttp://hdl.handle.net/10722/200704-
dc.description.abstractObjective Although Th17 cells have been increasingly recognised as an important effector in various autoimmune diseases, their function in the pathogenesis of Sjögren's syndrome (SS) remains largely uncharacterised. This study aims to determine the role of Th17 cells in the development of experimental SS (ESS). Methods The ESS was induced in wildtype and IL-17A knockout (IL-17 KO) C57BL/6 mice immunised with salivary glands (SG) proteins. Phenotypic analysis of immune cells in the draining cervical lymph nodes (CLN) and SG was performed by flow cytometry and immunofluorescence microscopy. To determine the role of Th17 cells in ESS, immunised IL-17 KO mice were adoptively transferred with in vitro-generated Th17 cells and monitored for SS development. The salivary flow rate was measured, whereas inflammatory infiltration and tissue destruction in SG were assessed by histopathology. Results SG protein-immunised mice developed overt SS symptoms with increased Th17 cells detected in CLN and within lymphocytic foci in inflamed SG. Notably, immunised IL-17 KO mice were completely resistant for SS induction, showing no evidence of disease symptoms and histopathological changes in SG. Adoptive transfer of Th17 cells rapidly induced the onset of ESS in immunised IL-17 KO mice with markedly reduced saliva secretion, elevated autoantibody production and pronounced inflammation and tissue damage in SG. Conclusions Our findings have defined a critical role of Th17 cells in the pathogenesis of ESS. Further studies may validate Th17 cell as a potential target for treating SS.-
dc.languageeng-
dc.publisherBMJ Publishing Group. The Journal's web site is located at http://ard.bmjjournals.com/-
dc.relation.ispartofAnnals of the Rheumatic Diseases-
dc.rightsAnnals of the Rheumatic Diseases. Copyright © B M J Publishing Group.-
dc.rightsThis article has been accepted for publication in Annals of the Rheumatic Diseases, 2015, v. 74 n. 6, p. 1302-1310 following peer review and can also be viewed on the journal’s website at http://ard.bmj.com/content/early/2014/02/26/annrheumdis-2013-204584-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleTh17 cells play a critical role in the development of experimental Sjogren's syndrome-
dc.typeArticle-
dc.identifier.emailLin, X: linxiang@hku.hk-
dc.identifier.emailChan, VSF: sfvchan@hku.hk-
dc.identifier.emailLau, WCS: cslau@hku.hk-
dc.identifier.emailLu, L: liweilu@hku.hk-
dc.identifier.authorityChan, VSF=rp01459-
dc.identifier.authorityLau, WCS=rp01348-
dc.identifier.authorityLu, L=rp00477-
dc.description.naturepostprint-
dc.identifier.doi10.1136/annrheumdis-2013-204584-
dc.identifier.pmid24573745-
dc.identifier.hkuros234536-
dc.identifier.volume74-
dc.identifier.issue6-
dc.publisher.placeUnited Kingdom-

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