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Conference Paper: Psr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindle

TitlePsr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindle
Authors
Issue Date2012
PublisherThe American Society for Cell Biology. The conference's website is located at https://www.ascb.org/index.php?option=com_content&view=article&id=596&Itemid=9
Citation
The 2012 Annual Meeting of the American Society for Cell Biology (ASCB), San Francisco, CA., 15-19 December 2012. How to Cite?
AbstractDuring mitosis, interpolar microtubules from two spindle pole bodies (SPBs) interdigitate to create an antiparallel microtubule array for accommodating numerous regulatory proteins. Among these proteins, the kinesin-5 cut7p/Eg5 is the key player responsible for sliding apart antiparallel microtubules and thus helps in establishing the bipolar spindle. At the onset of mitosis, two SPBs are adjacent to one another with most microtubules running nearly parallel toward the nuclear envelope, creating an unfavorable microtubule configuration for the kinesin-5 kinesins. Therefore, how the cell organizes the antiparallel microtubule array in the first place at mitotic onset remains enigmatic. Here, we show that a novel protein psrp1p localizes to the SPB and plays a key role in organizing the antiparallel microtubule array. The absence of psr1+ leads to a transient monopolar spindle and massive chromosome loss. Further functional characterization demonstrates that psr1p is recruited to the SPB through interaction with the conserved SUN protein sad1p and that psr1p physically interacts with the conserved microtubule plus tip protein mal3p/EB1. These results suggest a model that psr1p serves as a linking protein between sad1p/SUN and mal3p/EB1 to allow microtubule plus ends to be coupled to the SPBs for organization of an antiparallel microtubule array. Thus, we conclude that psr1p is involved in organizing the antiparallel microtubule array in the first place at mitosis onset by interaction with SUN/sad1p and EB1/mal3p, thereby establishing the bipolar spindle.
DescriptionRegular Abstracts - Sunday Poster Presentations: no. 382
Persistent Identifierhttp://hdl.handle.net/10722/189710

 

DC FieldValueLanguage
dc.contributor.authorFu, Cen_US
dc.contributor.authorScheffler, Ken_US
dc.contributor.authorSyrovaktina, Ven_US
dc.contributor.authorLi, Ten_US
dc.contributor.authorTran, Pen_US
dc.date.accessioned2013-09-17T14:55:52Z-
dc.date.available2013-09-17T14:55:52Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting of the American Society for Cell Biology (ASCB), San Francisco, CA., 15-19 December 2012.en_US
dc.identifier.urihttp://hdl.handle.net/10722/189710-
dc.descriptionRegular Abstracts - Sunday Poster Presentations: no. 382-
dc.description.abstractDuring mitosis, interpolar microtubules from two spindle pole bodies (SPBs) interdigitate to create an antiparallel microtubule array for accommodating numerous regulatory proteins. Among these proteins, the kinesin-5 cut7p/Eg5 is the key player responsible for sliding apart antiparallel microtubules and thus helps in establishing the bipolar spindle. At the onset of mitosis, two SPBs are adjacent to one another with most microtubules running nearly parallel toward the nuclear envelope, creating an unfavorable microtubule configuration for the kinesin-5 kinesins. Therefore, how the cell organizes the antiparallel microtubule array in the first place at mitotic onset remains enigmatic. Here, we show that a novel protein psrp1p localizes to the SPB and plays a key role in organizing the antiparallel microtubule array. The absence of psr1+ leads to a transient monopolar spindle and massive chromosome loss. Further functional characterization demonstrates that psr1p is recruited to the SPB through interaction with the conserved SUN protein sad1p and that psr1p physically interacts with the conserved microtubule plus tip protein mal3p/EB1. These results suggest a model that psr1p serves as a linking protein between sad1p/SUN and mal3p/EB1 to allow microtubule plus ends to be coupled to the SPBs for organization of an antiparallel microtubule array. Thus, we conclude that psr1p is involved in organizing the antiparallel microtubule array in the first place at mitosis onset by interaction with SUN/sad1p and EB1/mal3p, thereby establishing the bipolar spindle.-
dc.languageengen_US
dc.publisherThe American Society for Cell Biology. The conference's website is located at https://www.ascb.org/index.php?option=com_content&view=article&id=596&Itemid=9-
dc.relation.ispartofAnnual Meeting of the American Society for Cell Biology, ASCB 2012en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePsr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindleen_US
dc.typeConference_Paperen_US
dc.identifier.emailFu, C: chuanhai@hku.hken_US
dc.identifier.authorityFu, C=rp01515en_US
dc.description.naturepostprint-
dc.identifier.hkuros221643en_US
dc.publisher.placeUnited States-

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