File Download

There are no files associated with this item.

Supplementary

Conference Paper: Nucleic acids aptamers for malaria diagnosis - discovery, structure and application

TitleNucleic acids aptamers for malaria diagnosis - discovery, structure and application
Authors
Issue Date2013
PublisherIATI-BioMed.
Citation
The IATI-BioMed Israel 2013 Conference, Tel Aviv, Israel, 10-12 June 2013. How to Cite?
AbstractBetter malaria diagnosis approaches at the point of care are critically needed. Antibody-based rapid diagnostic tests have made a major positive impact on malaria management. However, cost, robustness, and challenges in manufacturing remain as issues innate to antibodies in tests suitable for widespread use in the developing world. We have been researching DNA aptamers against malaria diagnostic proteins that may underpin a change in the underlying molecular recognition technology for malaria diagnostic tests. Highly specific and tight-binding aptamers against a diagnostic protein target for pan-species malaria detection (lactate dehydrogenase) and a Plasmodium falciparum specific target (histidine rich protein II) were identified. We solved the X-ray crystal structure of a DNA aptamer in complex with its lactate dehydrogenase target, which elucidated the mechanism of discriminatory binding to Plasmodium over human lactate hydrogenase which we had optimized using counter selection techniques. We conjugated the aptamers to nanoparticles and have developed a colorimetric biosensor for the diagnostic targets. Research is ongoing based on this new technology to develop a diagnostic device suitable for clinical trials that we aim to be more sensitive, less expensive, more robust and easier to manufacture than the best antibody-based rapid diagnostic tests for malaria available today.
DescriptionCompanies and Academia Presentations
The Conference program's website is located at http://www2.kenes.com/BIOMED/CONFERENCE/Pages/Program_Glance.aspx
Persistent Identifierhttp://hdl.handle.net/10722/186629

 

DC FieldValueLanguage
dc.contributor.authorTanner, Jen_US
dc.date.accessioned2013-08-20T12:15:37Z-
dc.date.available2013-08-20T12:15:37Z-
dc.date.issued2013en_US
dc.identifier.citationThe IATI-BioMed Israel 2013 Conference, Tel Aviv, Israel, 10-12 June 2013.en_US
dc.identifier.urihttp://hdl.handle.net/10722/186629-
dc.descriptionCompanies and Academia Presentations-
dc.descriptionThe Conference program's website is located at http://www2.kenes.com/BIOMED/CONFERENCE/Pages/Program_Glance.aspx-
dc.description.abstractBetter malaria diagnosis approaches at the point of care are critically needed. Antibody-based rapid diagnostic tests have made a major positive impact on malaria management. However, cost, robustness, and challenges in manufacturing remain as issues innate to antibodies in tests suitable for widespread use in the developing world. We have been researching DNA aptamers against malaria diagnostic proteins that may underpin a change in the underlying molecular recognition technology for malaria diagnostic tests. Highly specific and tight-binding aptamers against a diagnostic protein target for pan-species malaria detection (lactate dehydrogenase) and a Plasmodium falciparum specific target (histidine rich protein II) were identified. We solved the X-ray crystal structure of a DNA aptamer in complex with its lactate dehydrogenase target, which elucidated the mechanism of discriminatory binding to Plasmodium over human lactate hydrogenase which we had optimized using counter selection techniques. We conjugated the aptamers to nanoparticles and have developed a colorimetric biosensor for the diagnostic targets. Research is ongoing based on this new technology to develop a diagnostic device suitable for clinical trials that we aim to be more sensitive, less expensive, more robust and easier to manufacture than the best antibody-based rapid diagnostic tests for malaria available today.-
dc.languageengen_US
dc.publisherIATI-BioMed.-
dc.relation.ispartofIATI-BioMed Israel 2013 Conferenceen_US
dc.titleNucleic acids aptamers for malaria diagnosis - discovery, structure and applicationen_US
dc.typeConference_Paperen_US
dc.identifier.emailTanner, J: jatanner@hku.hken_US
dc.identifier.authorityTanner, J=rp00495en_US
dc.identifier.hkuros217919en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats