File Download

There are no files associated with this item.

Supplementary

Conference Paper: The use of aptamer‐conjugated nanoparticles in novel malaria diagnosis platforms

TitleThe use of aptamer‐conjugated nanoparticles in novel malaria diagnosis platforms
Authors
Issue Date2013
Citation
Hong Kong Inter‐University Biochemistry Postgraduate Symposium, Hong Kong, China, 15 June 2013, abstract no. P29 How to Cite?
AbstractThe on‐going battle against malaria not only requires novel therapies to combat emerging resistant strains, but also requires effective point of care diagnosis so that such therapies can be appropriately administered, especially in underdeveloped regions where the disease is most prevalent. Current point‐of‐care rapid diagnostics rely on antibody‐based technologies which have drawbacks of cost and stability. Aptamers are oligonucleotide sequences which are developed to specifically bind desired targets, essentially DNA‐based antibodies. The stability and cheap manufacture cost of short DNA sequences make aptamers a viable alternative to antibodies in malaria diagnosis (aptamers are usually < 50 bases in length). The common malaria antigens which are detected by most malaria diagnosis blood tests are histidine‐rich protein 2 (HRP2) and Plasmodium falciparum lactose dehydrogenase (PvLDH). Aptamers for these antigens have been developed, characterised and found to bind with nanomolar affinities. Conjugation of these aptamersto gold and silver nanoparticles has allowed for visual detection of HRP2 and PvLDH in solution (owing to the visual properties of metal nanoparticles) and the next step of the project is to incorporate such aptamer‐ functionalised nanoparticles into a paper‐based lateral flow devices for future sensitive, cheap and easy‐to‐ use malaria diagnostics.  
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/186626

 

DC FieldValueLanguage
dc.contributor.authorDirkzwager, RMen_US
dc.contributor.authorCheung, YWen_US
dc.contributor.authorTanner, JAen_US
dc.date.accessioned2013-08-20T12:15:37Z-
dc.date.available2013-08-20T12:15:37Z-
dc.date.issued2013en_US
dc.identifier.citationHong Kong Inter‐University Biochemistry Postgraduate Symposium, Hong Kong, China, 15 June 2013, abstract no. P29en_US
dc.identifier.urihttp://hdl.handle.net/10722/186626-
dc.descriptionPoster Presentation-
dc.description.abstractThe on‐going battle against malaria not only requires novel therapies to combat emerging resistant strains, but also requires effective point of care diagnosis so that such therapies can be appropriately administered, especially in underdeveloped regions where the disease is most prevalent. Current point‐of‐care rapid diagnostics rely on antibody‐based technologies which have drawbacks of cost and stability. Aptamers are oligonucleotide sequences which are developed to specifically bind desired targets, essentially DNA‐based antibodies. The stability and cheap manufacture cost of short DNA sequences make aptamers a viable alternative to antibodies in malaria diagnosis (aptamers are usually < 50 bases in length). The common malaria antigens which are detected by most malaria diagnosis blood tests are histidine‐rich protein 2 (HRP2) and Plasmodium falciparum lactose dehydrogenase (PvLDH). Aptamers for these antigens have been developed, characterised and found to bind with nanomolar affinities. Conjugation of these aptamersto gold and silver nanoparticles has allowed for visual detection of HRP2 and PvLDH in solution (owing to the visual properties of metal nanoparticles) and the next step of the project is to incorporate such aptamer‐ functionalised nanoparticles into a paper‐based lateral flow devices for future sensitive, cheap and easy‐to‐ use malaria diagnostics.  -
dc.languageengen_US
dc.relation.ispartofHong Kong Inter‐University Biochemistry Postgraduate Symposiumen_US
dc.titleThe use of aptamer‐conjugated nanoparticles in novel malaria diagnosis platformsen_US
dc.typeConference_Paperen_US
dc.identifier.emailTanner, JA: jatanner@hku.hken_US
dc.identifier.authorityTanner, JA=rp00495en_US
dc.identifier.hkuros217913en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats