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Conference Paper: Polyphosphate regulates interleukin-11 levels of SaOS-2 cells

TitlePolyphosphate regulates interleukin-11 levels of SaOS-2 cells
Authors
Issue Date2013
Citation
Hong Kong Inter‐University Biochemistry Postgraduate Symposium, Hong Kong, China, 15 June 2013, abstract no. P24 How to Cite?
AbstractInorganic polyphoshate (polyP) is a linear polymer of orthophosphates (Pi) that varies in chain length from lessthan ten to several hundred residues. PolyP is a ubiquitous molecule in both prokaryotes and eukaryotes and has important physiological functions. Since its discovery in bacteria around 100 years ago, research has mainly been performed on prokaryotic functions with discoveries relating to polyphosphate function in survival and pathogen virulence. At present, the various functions of polyP in eukaryotes, especially in mammalian cells, are being elucidated. PolyP can play important roles in blood coagulation, inflammation, controlling mitochondrial calcium level thus affecting cell apoptosis and bone and cartilage formation. PolyP has been known to have relatively high concentrations in osteoblasts but the mechanisms for its action have been unknown. Here, we investigate the roles of polyP in signaling in cell culture studies of osteoblasts. We find polyP has significant influence on human‐osteoblast like SaoS‐2 cell proliferation and migration. Microarray studies showed a number of genes up and down‐regulated in response to polyP. In particular, we investigated interleukin‐11 in detail, and showed that interleukin‐11 is specifically upregulated at both RNA and protein levels in response to polyP, but not in response to orthophosphate, pyrophosphate or triphosphate, indicating specificity for long‐chain polyphosphate. Work is ongoing to unravel the crosstalk in signaling between inorganic polyphosphate and interleukin‐11.   
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/186625

 

DC FieldValueLanguage
dc.contributor.authorAo, KLen_US
dc.contributor.authorLui, LHEen_US
dc.contributor.authorLi, Len_US
dc.contributor.authorShum, KTen_US
dc.contributor.authorTanner, JAen_US
dc.date.accessioned2013-08-20T12:15:37Z-
dc.date.available2013-08-20T12:15:37Z-
dc.date.issued2013en_US
dc.identifier.citationHong Kong Inter‐University Biochemistry Postgraduate Symposium, Hong Kong, China, 15 June 2013, abstract no. P24en_US
dc.identifier.urihttp://hdl.handle.net/10722/186625-
dc.descriptionPoster Presentation-
dc.description.abstractInorganic polyphoshate (polyP) is a linear polymer of orthophosphates (Pi) that varies in chain length from lessthan ten to several hundred residues. PolyP is a ubiquitous molecule in both prokaryotes and eukaryotes and has important physiological functions. Since its discovery in bacteria around 100 years ago, research has mainly been performed on prokaryotic functions with discoveries relating to polyphosphate function in survival and pathogen virulence. At present, the various functions of polyP in eukaryotes, especially in mammalian cells, are being elucidated. PolyP can play important roles in blood coagulation, inflammation, controlling mitochondrial calcium level thus affecting cell apoptosis and bone and cartilage formation. PolyP has been known to have relatively high concentrations in osteoblasts but the mechanisms for its action have been unknown. Here, we investigate the roles of polyP in signaling in cell culture studies of osteoblasts. We find polyP has significant influence on human‐osteoblast like SaoS‐2 cell proliferation and migration. Microarray studies showed a number of genes up and down‐regulated in response to polyP. In particular, we investigated interleukin‐11 in detail, and showed that interleukin‐11 is specifically upregulated at both RNA and protein levels in response to polyP, but not in response to orthophosphate, pyrophosphate or triphosphate, indicating specificity for long‐chain polyphosphate. Work is ongoing to unravel the crosstalk in signaling between inorganic polyphosphate and interleukin‐11.   -
dc.languageengen_US
dc.relation.ispartofHong Kong Inter‐University Biochemistry Postgraduate Symposiumen_US
dc.titlePolyphosphate regulates interleukin-11 levels of SaOS-2 cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLi, L: linali@hku.hken_US
dc.identifier.emailTanner, JA: jatanner@hku.hken_US
dc.identifier.authorityTanner, JA=rp00495en_US
dc.identifier.hkuros217910en_US

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