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Article: Enhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infection

TitleEnhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infection
Authors
Issue Date2013
Citation
PLoS One, 2013, v. 8 n. 4, p. e61135 How to Cite?
AbstractDevelopment of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.
Persistent Identifierhttp://hdl.handle.net/10722/186447
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorFeng, Gen_US
dc.contributor.authorJiang, Qen_US
dc.contributor.authorXia, Men_US
dc.contributor.authorLu, Yen_US
dc.contributor.authorQiu, Wen_US
dc.contributor.authorZhao, Den_US
dc.contributor.authorLu, Len_US
dc.contributor.authorPeng, Gen_US
dc.contributor.authorWang, Yen_US
dc.date.accessioned2013-08-20T12:09:06Z-
dc.date.available2013-08-20T12:09:06Z-
dc.date.issued2013en_US
dc.identifier.citationPLoS One, 2013, v. 8 n. 4, p. e61135en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/186447-
dc.description.abstractDevelopment of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.-
dc.languageengen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleEnhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infectionen_US
dc.typeArticleen_US
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0061135-
dc.identifier.pmcidPMC3634041-
dc.identifier.hkuros218883en_US
dc.identifier.volume8en_US
dc.identifier.issue4en_US
dc.identifier.spagee61135en_US
dc.identifier.epagee61135en_US

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