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Article: Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line

TitlePolysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line
Authors
Issue Date2013
Citation
Inflammation Research, 2013, v. 62 n. 3, p. 313-324 How to Cite?
AbstractObjective: Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren's syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis. Materials: TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation. Results: We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors. Conclusions: Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors. © 2012 Springer Basel.
Persistent Identifierhttp://hdl.handle.net/10722/186043
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, Xen_US
dc.contributor.authorSze, CWen_US
dc.contributor.authorNg, TBen_US
dc.contributor.authorTong, Yen_US
dc.contributor.authorShaw, PCen_US
dc.contributor.authorTang, SCWen_US
dc.contributor.authorZhang, Yen_US
dc.date.accessioned2013-08-20T11:50:17Z-
dc.date.available2013-08-20T11:50:17Z-
dc.date.issued2013en_US
dc.identifier.citationInflammation Research, 2013, v. 62 n. 3, p. 313-324en_US
dc.identifier.urihttp://hdl.handle.net/10722/186043-
dc.description.abstractObjective: Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren's syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis. Materials: TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation. Results: We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors. Conclusions: Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors. © 2012 Springer Basel.-
dc.languageengen_US
dc.relation.ispartofInflammation Researchen_US
dc.titlePolysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell lineen_US
dc.typeArticleen_US
dc.identifier.emailSze, CW: stephens@hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.emailTang, SCW: scwtang@hku.hken_US
dc.identifier.emailZhang, Y: ybzhang@hku.hken_US
dc.identifier.authoritySze, CW=rp00514en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.identifier.authorityTang, SCW=rp00480en_US
dc.identifier.authorityZhang, Y=rp01410en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00011-012-0584-x-
dc.identifier.pmid23266598-
dc.identifier.scopuseid_2-s2.0-84877135473-
dc.identifier.hkuros219451en_US
dc.identifier.volume62en_US
dc.identifier.issue3en_US
dc.identifier.spage313en_US
dc.identifier.epage324en_US
dc.identifier.isiWOS:000314776000008-

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