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Conference Paper: The role of Sox10 in enteric neural crest cell migration

TitleThe role of Sox10 in enteric neural crest cell migration
Authors
Issue Date2013
PublisherThe Chinese University of Hong Kong.
Citation
The 2013 Hong Kong Inter-University Biochemistry Postgraduate Symposium, Hong Kong, 15 June 2013. In Symposium Booklet, 2013, abstract P40 How to Cite?
AbstractSox10 is a transcription factor required for enteric neural crest cells to colonize the gut during development. Sox10 functions to maintain enteric neural cell survival and multipotency. We have shown that Sox10 mutant enteric neural crest cells have abnormal migration trajectories with reduced migration speed and persistence in ex vivo gut culture, therefore we hypothesize that Sox10 is also required for enteric neural crest cell migration, possibly through regulating neural crest cell‐neural crest cell or neural crest‐extracellular matrix interactions. Here we aim to characterize how Sox10 influences the interaction between enteric neural crest cells and extracellular matrix molecules. We examined the behavior of enteric neural crest cells on ECM molecules by 1‐day ex vivo gut explant culture of E12.5 embryonic gut. Sox10NGFP/+ enteric neural crest cells were less dispersed and formed clusters on fibronectin and laminin substratum, indicating Sox10NGFP/+ enteric neural crest cells were unable to interact with extracellular matrix molecules in a normal manner. Analysis of time‐lapse videos capturing cell movements for 6 hours showed a significant reduction of speed and displacement in Sox10NGFP/+ enteric neural crest cells. We also showed by western blot analysis that pFAK397 was reduced in Sox10NGFP/+ guts, implying a role of Sox10 in regulating enteric neural crest cell motility. Altogether our findings point to a role of Sox10 in regulating enteric neural crest cell migration by modulating the interactions between enteric neural crest cells and extracellular matrix molecules.
Persistent Identifierhttp://hdl.handle.net/10722/184928

 

DC FieldValueLanguage
dc.contributor.authorSit, HMen_US
dc.contributor.authorZhang, Men_US
dc.contributor.authorLeung, Cen_US
dc.contributor.authorSham, MHen_US
dc.date.accessioned2013-07-15T10:18:26Z-
dc.date.available2013-07-15T10:18:26Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2013 Hong Kong Inter-University Biochemistry Postgraduate Symposium, Hong Kong, 15 June 2013. In Symposium Booklet, 2013, abstract P40en_US
dc.identifier.urihttp://hdl.handle.net/10722/184928-
dc.description.abstractSox10 is a transcription factor required for enteric neural crest cells to colonize the gut during development. Sox10 functions to maintain enteric neural cell survival and multipotency. We have shown that Sox10 mutant enteric neural crest cells have abnormal migration trajectories with reduced migration speed and persistence in ex vivo gut culture, therefore we hypothesize that Sox10 is also required for enteric neural crest cell migration, possibly through regulating neural crest cell‐neural crest cell or neural crest‐extracellular matrix interactions. Here we aim to characterize how Sox10 influences the interaction between enteric neural crest cells and extracellular matrix molecules. We examined the behavior of enteric neural crest cells on ECM molecules by 1‐day ex vivo gut explant culture of E12.5 embryonic gut. Sox10NGFP/+ enteric neural crest cells were less dispersed and formed clusters on fibronectin and laminin substratum, indicating Sox10NGFP/+ enteric neural crest cells were unable to interact with extracellular matrix molecules in a normal manner. Analysis of time‐lapse videos capturing cell movements for 6 hours showed a significant reduction of speed and displacement in Sox10NGFP/+ enteric neural crest cells. We also showed by western blot analysis that pFAK397 was reduced in Sox10NGFP/+ guts, implying a role of Sox10 in regulating enteric neural crest cell motility. Altogether our findings point to a role of Sox10 in regulating enteric neural crest cell migration by modulating the interactions between enteric neural crest cells and extracellular matrix molecules.-
dc.languageengen_US
dc.publisherThe Chinese University of Hong Kong.-
dc.relation.ispartofHong Kong Inter-University Biochemistry Postgraduate Symposium Booklet 2013en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleThe role of Sox10 in enteric neural crest cell migrationen_US
dc.typeConference_Paperen_US
dc.identifier.emailSit, HM: briansit@hku.hken_US
dc.identifier.emailZhang, M: zhangmei@hkusua.hku.hken_US
dc.identifier.emailLeung, C: leungcar@hkucc.hku.hken_US
dc.identifier.emailSham, MH: mhsham@hku.hk-
dc.identifier.authoritySham, MH=rp00380en_US
dc.description.naturepostprint-
dc.identifier.hkuros216503en_US
dc.publisher.placeHong Kong-

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