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Article: YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus

TitleYY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus
Authors
KeywordsB-Cell Development
Immunoglobulin
Polycomb
Rearrangement
Yy1
Issue Date2013
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.html
Citation
Embo Journal, 2013, v. 32 n. 8, p. 1168-1182 How to Cite?
AbstractConditional knock-out (KO) of Polycomb Group (PcG) protein YY1 results in pro-B cell arrest and reduced immunoglobulin locus contraction needed for distal variable gene rearrangement. The mechanisms that control these crucial functions are unknown. We deleted the 25 amino-acid YY1 REPO domain necessary for YY1 PcG function, and used this mutant (YY1ΔREPO), to transduce bone marrow from YY1 conditional KO mice. While wild-type YY1 rescued B-cell development, YY1ΔREPO failed to rescue the B-cell lineage yielding reduced numbers of B lineage cells. Although the IgH rearrangement pattern was normal, there was a selective impact at the Igκ locus that showed a dramatic skewing of the expressed Igκ repertoire. We found that the REPO domain interacts with proteins from the condensin and cohesin complexes, and that YY1, EZH2 and condensin proteins co-localize at numerous sites across the Ig kappa locus. Knock-down of a condensin subunit protein or YY1 reduced rearrangement of Igκ Vκ genes suggesting a direct role for YY1-condensin complexes in Igκ locus structure and rearrangement. © 2013 European Molecular Biology Organization.
Persistent Identifierhttp://hdl.handle.net/10722/184314
ISSN
2015 Impact Factor: 9.643
2015 SCImago Journal Rankings: 7.450
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPan, Xen_US
dc.contributor.authorPapasani, Men_US
dc.contributor.authorHao, Yen_US
dc.contributor.authorCalamito, Men_US
dc.contributor.authorWei, Fen_US
dc.contributor.authorQuinn, WJen_US
dc.contributor.authorBasu, Aen_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorHodawadekar, Sen_US
dc.contributor.authorZaprazna, Ken_US
dc.contributor.authorLiu, Hen_US
dc.contributor.authorShi, Yen_US
dc.contributor.authorAllman, Den_US
dc.contributor.authorCancro, Men_US
dc.contributor.authorAtchison, MLen_US
dc.date.accessioned2013-07-10T06:23:39Z-
dc.date.available2013-07-10T06:23:39Z-
dc.date.issued2013en_US
dc.identifier.citationEmbo Journal, 2013, v. 32 n. 8, p. 1168-1182en_US
dc.identifier.issn0261-4189en_US
dc.identifier.urihttp://hdl.handle.net/10722/184314-
dc.description.abstractConditional knock-out (KO) of Polycomb Group (PcG) protein YY1 results in pro-B cell arrest and reduced immunoglobulin locus contraction needed for distal variable gene rearrangement. The mechanisms that control these crucial functions are unknown. We deleted the 25 amino-acid YY1 REPO domain necessary for YY1 PcG function, and used this mutant (YY1ΔREPO), to transduce bone marrow from YY1 conditional KO mice. While wild-type YY1 rescued B-cell development, YY1ΔREPO failed to rescue the B-cell lineage yielding reduced numbers of B lineage cells. Although the IgH rearrangement pattern was normal, there was a selective impact at the Igκ locus that showed a dramatic skewing of the expressed Igκ repertoire. We found that the REPO domain interacts with proteins from the condensin and cohesin complexes, and that YY1, EZH2 and condensin proteins co-localize at numerous sites across the Ig kappa locus. Knock-down of a condensin subunit protein or YY1 reduced rearrangement of Igκ Vκ genes suggesting a direct role for YY1-condensin complexes in Igκ locus structure and rearrangement. © 2013 European Molecular Biology Organization.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.htmlen_US
dc.relation.ispartofEMBO Journalen_US
dc.subjectB-Cell Developmenten_US
dc.subjectImmunoglobulinen_US
dc.subjectPolycomben_US
dc.subjectRearrangementen_US
dc.subjectYy1en_US
dc.titleYY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locusen_US
dc.typeArticleen_US
dc.identifier.emailWang, J: junwen@hkucc.hku.hken_US
dc.identifier.authorityWang, J=rp00280en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/emboj.2013.66en_US
dc.identifier.pmid23531880-
dc.identifier.scopuseid_2-s2.0-84876419201en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84876419201&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32en_US
dc.identifier.issue8en_US
dc.identifier.spage1168en_US
dc.identifier.epage1182en_US
dc.identifier.isiWOS:000317748400011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPan, X=15036577200en_US
dc.identifier.scopusauthoridPapasani, M=6507375487en_US
dc.identifier.scopusauthoridHao, Y=55629671200en_US
dc.identifier.scopusauthoridCalamito, M=16201821700en_US
dc.identifier.scopusauthoridWei, F=55186434600en_US
dc.identifier.scopusauthoridQuinn, WJ=9232819800en_US
dc.identifier.scopusauthoridBasu, A=36672497400en_US
dc.identifier.scopusauthoridWang, J=8950599500en_US
dc.identifier.scopusauthoridHodawadekar, S=15044548300en_US
dc.identifier.scopusauthoridZaprazna, K=32868288900en_US
dc.identifier.scopusauthoridLiu, H=55631183200en_US
dc.identifier.scopusauthoridShi, Y=55630650100en_US
dc.identifier.scopusauthoridAllman, D=7006980158en_US
dc.identifier.scopusauthoridCancro, M=7004335899en_US
dc.identifier.scopusauthoridAtchison, ML=7006495633en_US

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