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Article: Application of SCAR (sequence characterized amplified region) analysis to authenticate Lycium barbarum (wolfberry) and its adulterants

TitleApplication of SCAR (sequence characterized amplified region) analysis to authenticate Lycium barbarum (wolfberry) and its adulterants
Authors
Issue Date2008
PublisherPortland Press Ltd. The Journal's web site is located at http://www.babonline.org
Citation
Biotechnology And Applied Biochemistry, 2008, v. 51 n. 1, p. 15-21 How to Cite?
AbstractFructus Lycii (Gouqizi) is well known in Chinese herbal medicine for its restorative function of benefiting the liver and kidney, replenishing vital essence and improving eyesight. However, ten species and varieties of Lycium have benn found to be substitutes or adulterants of Lycium barbarum (wolfberry) in commercial markets in the Hong Kong Special Administrative Region and in China generally. L. barbarum cv. 'Tianjinense' and Lycium chinense var. potaninii are the most common examples. It is difficult to differentiate among the Lycium species by traditional morphological and histological analyses. An easy and reliable approach based on SCAR (sequence characterized amplified region) analysis was developed in the present study to differentiate L. barbarum from other Lycium species. Two characteristic bands of approx. 700 and 650 bp were detected on the RAPD (random amplification of polymorphic DNA) profiles generated from samples of L. barbarum and L. chinense var. potaninii using the primer OPC-7. They were isolated and sequenced. Two primer sets, based on the sequences, could amplify a single specific band in samples of L. barbarum respectively, whereas no bands were detected in samples of L. chinense var. potaninii. The results confirmed that the SCAR technique can be employed for authenticating L. barbarum and its adulterants. © 2008 Portland Press Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/179448
ISSN
2015 Impact Factor: 1.429
2015 SCImago Journal Rankings: 0.415
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSze, SCWen_US
dc.contributor.authorSong, JXen_US
dc.contributor.authorWong, RNSen_US
dc.contributor.authorFeng, YBen_US
dc.contributor.authorNg, TBen_US
dc.contributor.authorTong, Yen_US
dc.contributor.authorZhang, KYBen_US
dc.date.accessioned2012-12-19T09:56:39Z-
dc.date.available2012-12-19T09:56:39Z-
dc.date.issued2008en_US
dc.identifier.citationBiotechnology And Applied Biochemistry, 2008, v. 51 n. 1, p. 15-21en_US
dc.identifier.issn0885-4513en_US
dc.identifier.urihttp://hdl.handle.net/10722/179448-
dc.description.abstractFructus Lycii (Gouqizi) is well known in Chinese herbal medicine for its restorative function of benefiting the liver and kidney, replenishing vital essence and improving eyesight. However, ten species and varieties of Lycium have benn found to be substitutes or adulterants of Lycium barbarum (wolfberry) in commercial markets in the Hong Kong Special Administrative Region and in China generally. L. barbarum cv. 'Tianjinense' and Lycium chinense var. potaninii are the most common examples. It is difficult to differentiate among the Lycium species by traditional morphological and histological analyses. An easy and reliable approach based on SCAR (sequence characterized amplified region) analysis was developed in the present study to differentiate L. barbarum from other Lycium species. Two characteristic bands of approx. 700 and 650 bp were detected on the RAPD (random amplification of polymorphic DNA) profiles generated from samples of L. barbarum and L. chinense var. potaninii using the primer OPC-7. They were isolated and sequenced. Two primer sets, based on the sequences, could amplify a single specific band in samples of L. barbarum respectively, whereas no bands were detected in samples of L. chinense var. potaninii. The results confirmed that the SCAR technique can be employed for authenticating L. barbarum and its adulterants. © 2008 Portland Press Ltd.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.babonline.orgen_US
dc.relation.ispartofBiotechnology and Applied Biochemistryen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDrugs, Chinese Herbalen_US
dc.subject.meshLycium - Chemistry - Genetics - Ultrastructureen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlants, Medicinal - Chemistry - Genetics - Ultrastructureen_US
dc.subject.meshRandom Amplified Polymorphic Dna Technique - Methodsen_US
dc.titleApplication of SCAR (sequence characterized amplified region) analysis to authenticate Lycium barbarum (wolfberry) and its adulterantsen_US
dc.typeArticleen_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.emailFeng, YB: yfeng@hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.emailZhang, KYB: ybzhang@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.identifier.authorityFeng, YB=rp00466en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.identifier.authorityZhang, KYB=rp01410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1042/BA20070096en_US
dc.identifier.pmid18052933-
dc.identifier.scopuseid_2-s2.0-51149098078en_US
dc.identifier.hkuros145916-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51149098078&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume51en_US
dc.identifier.issue1en_US
dc.identifier.spage15en_US
dc.identifier.epage21en_US
dc.identifier.isiWOS:000259131100003-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridSong, JX=24339343800en_US
dc.identifier.scopusauthoridWong, RNS=7402126957en_US
dc.identifier.scopusauthoridFeng, YB=24467969600en_US
dc.identifier.scopusauthoridNg, TB=35311803300en_US
dc.identifier.scopusauthoridTong, Y=9045384000en_US
dc.identifier.scopusauthoridZhang, KYB=23483121900en_US

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