Stable heterologous propagation CFTR protein variant cDNA

Abstract

PCT No. PCT/CA91/00341 Sec. 371 Date Apr. 12, 1993 Sec. 102(e) Date Apr. 12, 1993 PCT Filed Sep. 20, 1991 PCT Pub. No. WO92/05252 PCT Pub. Date Apr. 2, 1992A modified DNA sequence encoding full length cystic fibrosis transmembrane conductance regulator protein is provided to facilitate propagation and/or expression of the protein in living cells and in particular, bacterial cells. The modified DNA sequence comprises at least one of the 13 base pair repeat of exon 6b of the normal gene encoding the conductance regulator protein, as one or more normal nucleotides of the 13 base repeat substituted with an alternate nucleotide which, however, continues to code for the corresponding normal amino acid. Mammalian cells transfected with a vector containing the modified DNA sequence enhances chlorine conductance through the cell wall.