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  Patent History
  • Application
    US 10/449801 2003-05-29
  • Publication
    US 2004058314 2004-03-25
  Patent Family
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published patent: Assay for the detection and quantification of HBV cccDNA by real-time PCR

TitleAssay for the detection and quantification of HBV cccDNA by real-time PCR
Priority Date2003-05-29 US 10/449801
2002-05-29 US 10/383953P
Inventors
Issue Date2004
Citation
US Published patent application US 2004058314. Washington, DC: US Patent and Trademark Office (USPTO), 2004 How to Cite?
AbstractThe persistence of covalently closed circular (ccc) DNA of Hepatitis B Virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. A set of primers were designed to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100 to 10<7 >copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy.
Persistent Identifierhttp://hdl.handle.net/10722/176862

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:38:36Z-
dc.date.available2012-11-30T08:38:36Z-
dc.date.issued2004-
dc.identifier.citationUS Published patent application US 2004058314. Washington, DC: US Patent and Trademark Office (USPTO), 2004en_HK
dc.identifier.urihttp://hdl.handle.net/10722/176862-
dc.description.abstractThe persistence of covalently closed circular (ccc) DNA of Hepatitis B Virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. A set of primers were designed to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100 to 10<7 >copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy.en_HK
dc.relation.isreferencedbyEP 2396428 (A4) 2012-07-25en_HK
dc.relation.isreferencedbyEP 2396428 (A2) 2011-12-21en_HK
dc.relation.isreferencedbyJP 2006217864 (A) 2006-08-24en_HK
dc.relation.isreferencedbyJP 4708045 (B2) 2011-06-22en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleAssay for the detection and quantification of HBV cccDNA by real-time PCRen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.hkuros92190-
dc.contributor.inventorHe Ming Liangen_HK
dc.contributor.inventorKung Hsiang-Fuen_HK
dc.contributor.inventorLin, MCen_HK
patents.identifier.applicationUS 10/449801en_HK
patents.description.assigneeHE MING LIANG, ; KUNG HSIANG-FU, ; LIN MARIE CHIA MIen_HK
patents.description.countryUnited States of Americaen_HK
patents.date.publication2004-03-25en_HK
patents.date.application2003-05-29en_HK
patents.date.priority2003-05-29 US 10/449801en_HK
patents.date.priority2002-05-29 US 10/383953Pen_HK
patents.description.ccUSen_HK
patents.identifier.publicationUS 2004058314en_HK
patents.relation.familyUS 2004058314 (A1) 2004-03-25en_HK
patents.description.kindA1en_HK
patents.typePatent_publisheden_HK

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